高温胁迫下景宁木兰淀粉与蔗糖代谢途径转录分析

郑晨璐; 王倩颖; 陆丹迎; 申亚梅; 马晶晶; 刘璐; 王云; 郑晨璐; 王倩颖; 陆丹迎; 申亚梅; 马晶晶; 刘璐; 王云

doi:10.11833/j.issn.2095-0756.20220170

[1]

郝力慧, 董彬, 朱绍华, 等. 牡丹响应高温胁迫的转录组分析及PsHSP基因表达[J]. 浙江农林大学学报, 2021, 38(4): 802 − 811.

HAO Lihui, DONG Bin, ZHU Shaohua, et al. Transcriptome analysis and PsHSP gene expression of Paeonia suffruticosa in response to high temperature stress [J]. Journal of Zhejiang A&F University, 2021, 38(4): 802 − 811.

[2]

许大全. 光合作用[M]. 北京: 科学出版社, 2013.

XU Daquan. Photosynthesis[M]. Beijing: Science Press, 2013.

[3]

DAIE J. Cytosolic fructose-1, 6-bisphosphatase: a key enzyme inthe sucrose biosynthetic pathway [J]. Photosynthesis Research., 1993, 38(1): 5 − 14.

[4]

SERRTO A, de DIOSBARAJASLOPE Z J, CHUECA A, et al. Changing sugar partitioning in FBPase-manipulated plants [J]. Journal of Experimental Botany, 2009, 60(10): 2923 − 2931.

[5]

王宁宁, 朱建新, 王淑芳, 等. 苦参碱对小麦旗叶中蔗糖磷酸合成酶活性的调节[J]. 南开大学学报(自然科学版), 2000, 33(1): 19 − 22.

WANG Ningning, ZHU Jianxin, WANG Shufang, et al. The effect of matrine on sucrose biosynthesis in wheat flag leaves [J]. Acta Scientiarum Naturalium Universitatis Nankaiensis, 2000, 33(1): 19 − 22.

[6]

HUBER S C. Role of sucrose-phosphate synthase in partitioning of carbon in leaves [J]. Plant Physiology, 1983, 71(4): 818 − 821.

[7]

李梓铭, 泮仪晨, 范小平, 等. 浙贝母淀粉、蔗糖代谢相关基因的克隆与表达分析[J]. 核农学报, 2021, 35(11): 2470 − 2481.

LI Zhiming, PAN Yichen, FAN Xiaoping, et al. Cloning and expression analysis of genes related to starch and sucrose metabolism in Fritillaria thunbergii[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(11): 2470 − 2481.

[8]

BALLICORA M A, IGLESIAS A A, PREISS J. ADP-glucose pyrophosphorylase: a regulatory enzyme for plant starch synthesis [J]. Photosynthesis Research, 2004, 79: 1 − 24.

[9]

王倩颖, 常鹏杰, 申亚梅, 等. 景宁木兰热胁迫下实时荧光定量 PCR 内参基因的筛选[J]. 浙江农林大学学报, 2019, 36(5): 935 − 942.

WANG Qianying, CHANG Pengjie, SHEN Yamei, et al. Reference genes for quantitative PCR in Magnolia sinostellata with heat stress [J]. Journal of Zhejiang A&F University, 2019, 36(5): 935 − 942.

[10]

BUYSSE J, MERCKX R. An improved colorimetric method to quantify sugar content of plant tissue [J]. Journal of Experimental Botany, 1993, 44(10): 1627 − 1629.

[11]

CHEN L S, CHENG L. Carbon assimilation and carbohydrate metabolism of ‘Concord’ grape (Vitis labrusca L. ) leaves in response to nitrogen supply [J]. Journal of the American Society for Horticultural Science, 2003, 128(5): 754 − 760.

[12]

GROFC P, KNIGHTD P, MCNEILSD, et al. A modified assay method shows leaf sucrose-phosphate synthase activity is correlated with leaf sucrose content across a range of sugarcane varieties [J]. Functional Plant Biology, 1998, 25(4): 499 − 502.

[13]

LI B, DEWEY C N. RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome[J/OL]. BMC Bioinformatics, 2011, 12(1): 323 [2022-01-17]. doi: 10.1186/1471-2105-12-323.

[14]

WANF W, VINOCUR B, ALTMAN A. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance [J]. Planta, 2003, 218(1): 1 − 14.

[15]

YE Jia, FANG Lin, ZHENG Hongkun, et al. WEGO: a web tool for plotting GO annotations [J]. Nucleic Acids Research, 2006, 34(2): 293 − 297.

[16]

祝小云. 切花非洲菊苗期在高温胁迫中的生理生化响应和转录组分析[D]. 杭州: 浙江农林大学, 2016.

ZHU Xiaoyun. Physiological and Biochemical Responses of Gerbera Cultivars to Heat Stress and Transcriptome Analysis[D]. Hangzhou: Zhejiang A&F University, 2016.

[17]

TAIZ L, ZEIGER E. 植物生理学[M]. 4版. 宋纯鹏, 王学路, 译. 北京: 科学出版社, 2015.

TAIZ L, ZEIGER E. Plant Physiology[M]. 4th ed. SONG Chunpeng, WANG Xuelu, tran. Beijing: Science Press, 2015.

[18]

THORBGORNSEN T, ASP T, JORGENSEN K, et al. Starch biosynthesis from triose-phosphate in transgenic potato tubers expressing plastidic fructose-1, 6-bisphosphatase [J]. Planta, 2002, 214(4): 616 − 624.

[19]

STRANDÅ, ZRENNER R, TREVANION S, et al. Decreased expression of two key enzymes in the sucrose biosynthesis pathway, cytosolic fructose-1, 6-bisphosphatase and sucrose phosphate synthase, has remarkably different consequences for photosynthetic carbon metabolism in transgenic Arabidopsis thaliana [J]. The Plant Journal, 2000, 23(6): 759 − 770.

[20]

HARBRON S, FOYER C, WALKER D. The purification and properties of sucrose-phosphate synthetase from spinach leaves: the involvement of this enzyme and fructose bisphosphatase in the regulation of sucrose biosynthesis [J]. Archives of Biochemistry and Biophysics, 1981, 212(1): 237 − 246.

[21]

刘凌霄, 沈法富, 卢合全, 等. 蔗糖代谢中蔗糖磷酸合成酶(SPS)的研究进展[J]. 分子植物育种, 2005, 3(2): 275 − 281.

LIU Lingxiao, SHEN Fafu, LU Hequan, et al. Research advance on sucrose phosphate synthase in sucrose metabolism[J]. Molecular Plant Breeding, 3(2): 275 − 281.

[22]

赵智中, 张上隆, 徐昌杰, 等. 蔗糖代谢相关酶在温州蜜柑果实糖积累中的作用[J]. 园艺学报, 2001, 28(2): 112 − 118.

ZHAO Zhizhong, ZHANG Shanglong, XU Changjie, et al. Roles of sucrose-metabolizing enzymes in accumulation of sugars in satsuma mandarin fruit [J]. Acta Horticulturae Sinica, 2001, 28(2): 112 − 118.

[23]

李天, 刘奇华, 大杉, 等. 灌浆结实期高温对水稻籽粒蔗糖及降解酶活性的影响[J]. 中国水稻科学, 2006, 20(6): 626 − 630.

LI Tian, LIU Qihua, RYU O, et al. Effect of high temperature on sucrose content and sucrose-cleaving enzymes activity in rice during grain filling stage [J]. Chinese Journal of Rice Science, 2006, 20(6): 626 − 630.

[24]

CHATTERTONN J, SILVIUSJ E. Photosynthate partitioning into starch in soybean leaves ‘Ⅰ’. Effects of photoperiod versus photosynthetic period duration [J]. Plant Physiology, 1979, 64(5): 749 − 753.

[25]

郭金妹, 李天来, 姜晶, 等. 昼间亚高温下番茄叶中糖含量与蔗糖代谢相关酶的活性日变化[J]. 植物生理学通讯, 2007, 43(2): 231 − 234.

GUO Jinmei, LI Tianlai, JIANG Jing, et al. Diurnal changes in sugar contents and enzymes activities involving sucrose metabolism in tomato (Lycopersicon esculentum Mill) leaves under sub-high temperature by day [J]. Plant Physiology Communications, 2007, 43(2): 231 − 234.

[1]	郝力慧, 董彬, 朱绍华, 等. 牡丹响应高温胁迫的转录组分析及PsHSP基因表达[J]. 浙江农林大学学报, 2021, 38(4): 802 − 811. HAO Lihui, DONG Bin, ZHU Shaohua, et al. Transcriptome analysis and PsHSP gene expression of Paeonia suffruticosa in response to high temperature stress [J]. Journal of Zhejiang A&F University, 2021, 38(4): 802 − 811.
[2]	许大全. 光合作用[M]. 北京: 科学出版社, 2013. XU Daquan. Photosynthesis[M]. Beijing: Science Press, 2013.
[3]	DAIE J. Cytosolic fructose-1, 6-bisphosphatase: a key enzyme inthe sucrose biosynthetic pathway [J]. Photosynthesis Research., 1993, 38(1): 5 − 14.
[4]	SERRTO A, de DIOSBARAJASLOPE Z J, CHUECA A, et al. Changing sugar partitioning in FBPase-manipulated plants [J]. Journal of Experimental Botany, 2009, 60(10): 2923 − 2931.
[5]	王宁宁, 朱建新, 王淑芳, 等. 苦参碱对小麦旗叶中蔗糖磷酸合成酶活性的调节[J]. 南开大学学报(自然科学版), 2000, 33(1): 19 − 22. WANG Ningning, ZHU Jianxin, WANG Shufang, et al. The effect of matrine on sucrose biosynthesis in wheat flag leaves [J]. Acta Scientiarum Naturalium Universitatis Nankaiensis, 2000, 33(1): 19 − 22.
[6]	HUBER S C. Role of sucrose-phosphate synthase in partitioning of carbon in leaves [J]. Plant Physiology, 1983, 71(4): 818 − 821.
[7]	李梓铭, 泮仪晨, 范小平, 等. 浙贝母淀粉、蔗糖代谢相关基因的克隆与表达分析[J]. 核农学报, 2021, 35(11): 2470 − 2481. LI Zhiming, PAN Yichen, FAN Xiaoping, et al. Cloning and expression analysis of genes related to starch and sucrose metabolism in Fritillaria thunbergii[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(11): 2470 − 2481.
[8]	BALLICORA M A, IGLESIAS A A, PREISS J. ADP-glucose pyrophosphorylase: a regulatory enzyme for plant starch synthesis [J]. Photosynthesis Research, 2004, 79: 1 − 24.
[9]	王倩颖, 常鹏杰, 申亚梅, 等. 景宁木兰热胁迫下实时荧光定量 PCR 内参基因的筛选[J]. 浙江农林大学学报, 2019, 36(5): 935 − 942. WANG Qianying, CHANG Pengjie, SHEN Yamei, et al. Reference genes for quantitative PCR in Magnolia sinostellata with heat stress [J]. Journal of Zhejiang A&F University, 2019, 36(5): 935 − 942.
[10]	BUYSSE J, MERCKX R. An improved colorimetric method to quantify sugar content of plant tissue [J]. Journal of Experimental Botany, 1993, 44(10): 1627 − 1629.
[11]	CHEN L S, CHENG L. Carbon assimilation and carbohydrate metabolism of ‘Concord’ grape (Vitis labrusca L. ) leaves in response to nitrogen supply [J]. Journal of the American Society for Horticultural Science, 2003, 128(5): 754 − 760.
[12]	GROFC P, KNIGHTD P, MCNEILSD, et al. A modified assay method shows leaf sucrose-phosphate synthase activity is correlated with leaf sucrose content across a range of sugarcane varieties [J]. Functional Plant Biology, 1998, 25(4): 499 − 502.
[13]	LI B, DEWEY C N. RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome[J/OL]. BMC Bioinformatics, 2011, 12(1): 323 [2022-01-17]. doi: 10.1186/1471-2105-12-323.
[14]	WANF W, VINOCUR B, ALTMAN A. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance [J]. Planta, 2003, 218(1): 1 − 14.
[15]	YE Jia, FANG Lin, ZHENG Hongkun, et al. WEGO: a web tool for plotting GO annotations [J]. Nucleic Acids Research, 2006, 34(2): 293 − 297.
[16]	祝小云. 切花非洲菊苗期在高温胁迫中的生理生化响应和转录组分析[D]. 杭州: 浙江农林大学, 2016. ZHU Xiaoyun. Physiological and Biochemical Responses of Gerbera Cultivars to Heat Stress and Transcriptome Analysis[D]. Hangzhou: Zhejiang A&F University, 2016.
[17]	TAIZ L, ZEIGER E. 植物生理学[M]. 4版. 宋纯鹏, 王学路, 译. 北京: 科学出版社, 2015. TAIZ L, ZEIGER E. Plant Physiology[M]. 4th ed. SONG Chunpeng, WANG Xuelu, tran. Beijing: Science Press, 2015.
[18]	THORBGORNSEN T, ASP T, JORGENSEN K, et al. Starch biosynthesis from triose-phosphate in transgenic potato tubers expressing plastidic fructose-1, 6-bisphosphatase [J]. Planta, 2002, 214(4): 616 − 624.
[19]	STRANDÅ, ZRENNER R, TREVANION S, et al. Decreased expression of two key enzymes in the sucrose biosynthesis pathway, cytosolic fructose-1, 6-bisphosphatase and sucrose phosphate synthase, has remarkably different consequences for photosynthetic carbon metabolism in transgenic Arabidopsis thaliana [J]. The Plant Journal, 2000, 23(6): 759 − 770.
[20]	HARBRON S, FOYER C, WALKER D. The purification and properties of sucrose-phosphate synthetase from spinach leaves: the involvement of this enzyme and fructose bisphosphatase in the regulation of sucrose biosynthesis [J]. Archives of Biochemistry and Biophysics, 1981, 212(1): 237 − 246.
[21]	刘凌霄, 沈法富, 卢合全, 等. 蔗糖代谢中蔗糖磷酸合成酶(SPS)的研究进展[J]. 分子植物育种, 2005, 3(2): 275 − 281. LIU Lingxiao, SHEN Fafu, LU Hequan, et al. Research advance on sucrose phosphate synthase in sucrose metabolism[J]. Molecular Plant Breeding, 3(2): 275 − 281.
[22]	赵智中, 张上隆, 徐昌杰, 等. 蔗糖代谢相关酶在温州蜜柑果实糖积累中的作用[J]. 园艺学报, 2001, 28(2): 112 − 118. ZHAO Zhizhong, ZHANG Shanglong, XU Changjie, et al. Roles of sucrose-metabolizing enzymes in accumulation of sugars in satsuma mandarin fruit [J]. Acta Horticulturae Sinica, 2001, 28(2): 112 − 118.
[23]	李天, 刘奇华, 大杉, 等. 灌浆结实期高温对水稻籽粒蔗糖及降解酶活性的影响[J]. 中国水稻科学, 2006, 20(6): 626 − 630. LI Tian, LIU Qihua, RYU O, et al. Effect of high temperature on sucrose content and sucrose-cleaving enzymes activity in rice during grain filling stage [J]. Chinese Journal of Rice Science, 2006, 20(6): 626 − 630.
[24]	CHATTERTONN J, SILVIUSJ E. Photosynthate partitioning into starch in soybean leaves ‘Ⅰ’. Effects of photoperiod versus photosynthetic period duration [J]. Plant Physiology, 1979, 64(5): 749 − 753.
[25]	郭金妹, 李天来, 姜晶, 等. 昼间亚高温下番茄叶中糖含量与蔗糖代谢相关酶的活性日变化[J]. 植物生理学通讯, 2007, 43(2): 231 − 234. GUO Jinmei, LI Tianlai, JIANG Jing, et al. Diurnal changes in sugar contents and enzymes activities involving sucrose metabolism in tomato (Lycopersicon esculentum Mill) leaves under sub-high temperature by day [J]. Plant Physiology Communications, 2007, 43(2): 231 − 234.