百合原生质体分离与培养研究进展

陈兴旺; 韩炘; 陈敏敏; 杨云尧; 聂功平; 张永春; 明凤; 杨柳燕; 陈兴旺; 韩炘; 陈敏敏; 杨云尧; 聂功平; 张永春; 明凤; 杨柳燕

doi:10.11833/j.issn.2095-0756.20250422

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[1]	程金水, 刘青林. 园林植物遗传育种学[M]. 2版. 北京: 中国林业出版社, 2010: 340−350. CHENG Jinshui, LIU Qinglin. Genetics and Breeding of Garden Plants[M]. 2nd ed. Beijing: China Forestry Publishing House, 2010: 340−350.
[2]	CHEN Minmin, YANG Yunyao, HAN Xin, et al. Metabolomics integrated with transcriptomics provides insights into the phenylpropanoids biosynthesis pathway in Lilium davidii var. unicolor and L. lancifolium Thunb. [J/OL]. International Journal of Biological Macromolecules, 2024, 279(1): 135103[2025-07-05]. DOI: 10.1016/j.ijbiomac.2024.135103.
[3]	马莉. 百合属杂交育种的研究[D]. 北京: 北京林业大学, 2008. MA Li. Research of Cross-breeding of Lilium [D]. Beijing: Beijing Forestry University, 2008.
[4]	韩炘, 杨柳燕, 陈敏敏, 等. 百合育种技术研究进展[J]. 植物遗传资源学报, 2024, 25(5): 763−776. HAN Xin, YANG Liuyan, CHEN Minmin, et al. Research progress of the techniques applicable in lily breeding [J]. Journal of Plant Genetic Resources, 2024, 25(5): 763−776.
[5]	MUKUNDAN N S, SATYAMOORTHY K, BABU V S. Advancing plant protoplasts: innovative techniques and future prospects[J/OL]. Plant Biotechnology Reports, 2025[2025-07-05]. DOI: 10.1007/s11816-025-00957-1.
[6]	COCKING E C. A method for the isolation of plant protoplasts and vacuoles [J]. Nature, 1960, 187(4741): 962−963.
[7]	NAGATA T, TAKEBE I. Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts [J]. Planta, 1970, 92(4): 301−308.
[8]	LIU Shuping, LI Xiaojie, ZHU Jiani, et al. Modern technologies provide new opportunities for somatic hybridization in the breeding of woody plants[J/OL]. Plants, 2024, 13(18): 2539[2025-07-05]. DOI: 10.3390/plants13182539.
[9]	MOON K B, PARK J S, PARK S J, et al. A more accessible, time-saving, and efficient method for in vitro plant regeneration from potato protoplasts[J/OL]. Plants, 2021, 10(4): 781[2025-07-05]. DOI: 10.3390/plants10040781.
[10]	CAI Xiaodong, FU Jing, GUO Wenwu. Mitochondrial genome of callus protoplast has a role in mesophyll protoplast regeneration in Citrus: evidence from transgenic GFP somatic Homo-fusion [J]. Horticultural Plant Journal, 2017, 3(5): 177−182.
[11]	KAZMI S K, KAYANI H A, RAZIQ M, et al. Protoplast isolation and regeneration from leaves and nucellar embryos of Kinnow mandarin (Citrus reticulata Blanco)[J/OL]. Pakistan Journal of Botany, 2022, 54(4)[2025-07-05]. DOI: 10.30848/pjb2022-4(27).
[12]	YAJI C, KAWAGUCHI R, YASUHARA H. Caffeine inhibits cell-wall regeneration in protoplasts isolated from tobacco BY-2 cells [J]. Cytologia, 2023, 88(4): 313−319.
[13]	赵小强, 鹿金颖, 陈瑜, 等. 马铃薯原生质体培养与体细胞杂交研究进展[J]. 江苏农业科学, 2020, 48(22): 6−14. ZHAO Xiaoqiang, LU Jinying, CHEN Yu, et al. Research progress on protoplast culture and somatic hybridization of potato [J]. Jiangsu Agricultural Sciences, 2020, 48(22): 6−14.
[14]	章志宏, 张晓国, 朱英国. 应用生物技术创建植物雄性不育新种质[J]. 武汉植物学研究, 1999, 17(增刊): 45−51. ZHANG Zhihong, ZHANG Xiaoguo, ZHU Yingguo. Using biotechnology to construct new male sterile germplasm of plant [J]. Journal of Wuhan Botanical Research, 1999, 17(suppl): 45−51.
[15]	解凯东, 方燕妮, 伍小萌, 等. 无核柚新品种‘华柚2号’[J]. 园艺学报, 2020, 47(S2): 2946−2947. XIE Kaidong, FANG Yanni, WU Xiaomeng, et al. A new seedless pummelo cultivar‘Huayou 2’ [J]. Acta Horticulturae Sinica, 2020, 47(S2): 2946−2947.
[16]	解凯东, 伍小萌, 方燕妮, 等. 无核柚新品种‘华柚3号’[J]. 园艺学报, 2021, 48(增刊2): 2815−2816. XIE Kaidong, WU Xiaomeng, FANG Yanni, et al. A new seedless pummelo cultivar ‘Huayou 3’ [J]. Acta Horticulturae Sinica, 2021, 48(suppl 2): 2815−2816.
[17]	何珊珊, 李宏宇, 马月, 等. 百合原生质体分离培养和瞬时转化[J]. 浙江大学学报(农业与生命科学版), 2025, 51(1): 67−79. HE Shanshan, LI Hongyu, MA Yue, et al. Isolation, cultivation, and transient transformation of lily protoplasts [J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2025, 51(1): 67−79.
[18]	HORITA M, MOROHASHI H, KOMAI F. Regeneration of flowering plants from difficile lily protoplasts by means of a nurse culture [J]. Planta, 2002, 215(5): 880−884.
[19]	SIMMONDS J A, SIMMONDS D H, CUMMING B G. Isolation and cultivation of protoplasts from morphogenetic callus cultures of Lilium [J]. Canadian Journal of Botany, 1979, 57(5): 512−516.
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