[1] 肖建华, 丁鑫, 蔡超男, 等. 闽楠(Phoebe bournei, Lauraceae)地理分布及随气候变化的分布格局模拟[J]. 生态学报, 2021, 41(14): 5703−5712.

XIAO Jianhua, DING Xin, CAI Chaonan, et al. Simulation of the potential distribution of Phoebe bournei with climate changes using the maximum-entropy (MaxEnt) model[J]. Acta Ecologica Sinica, 2021, 41(14): 5703−5712.
[2] 尹耀南. 闽楠生长特性及其苗木培育技术[J]. 林业与生态, 2023(9): 42−43.

YIN Yaonan. Growth characteristics of Phoebe bournei and its seedling cultivation technology[J] Forestry and Ecology, 2023(9): 42−43.
[3] 曹苜, 刘刚. 闽楠研究进展[J]. 长江大学学报(自然科学版), 2016, 13(27): 1−3, 27.

CAO Mu, LIU Gang. Research progress of Phoebe bournei[J]. Journal of Yangtze University (Natural Science Edition), 2016, 13(27): 1−3, 27.
[4] HAN Xiao, ZHANG Junhong, HAN Shuang, et al. The chromosome-scale genome of Phoebe bournei reveals contrasting fates of terpene synthase (TPS)-a and TPS-b subfamilies[J/OL]. Plant Communications, 2022, 3(6): 100410[2024-09-30]. DOI: 10.1016/j.xplc.2022.100410.
[5] XU Wenting, ZHANG Miao, WANG Chen, et al. Somatic embryo induction and Agrobacterium-mediated transformation of embryonic callus tissue in Phoebe bournei, an endangered woody species in Lauraceae[J]. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2020, 48(2): 572−587.
[6] ZHANG Miao, CHEN Xinyi, LOU Xiongzhen, et al. Identification of WUSCHEL-related homeobox (WOX) gene family members and determination of their expression profiles during somatic embryogenesis in Phoebe bournei[J/OL]. Forestry Research, 2023, 3: 5[2024-09-30]. DOI: 10.48130/FR-2023-0005.
[7] 吴梦洁, 洪家都, 李芳燕, 等. 发根农杆菌介导的闽楠遗传转化体系构建与优化[J]. 核农学报, 2023, 37(8): 1516−1522.

WU Mengjie, HONG Jiadu, LI Fangyan, et al. Construction and optimization of genetic transformation system mediated by Agrobacterium rhizogenes in Phoebe bournei[J]. Journal of Nuclear Agricultural Sciences, 2023, 37(8): 1516−1522.
[8] XU Ying, LI Ruilian, LUO Hongbing, et al. Protoplasts: small cells with big roles in plant biology[J]. Trends in Plant Science, 2022, 27(8): 828−829.
[9] REYNA-LLORENS I, FERRO-COSTA M, BURGESS S J. Plant protoplasts in the age of synthetic biology[J]. Journal of Experimental Botany, 2023, 74(13): 3821−3832.
[10] DAVEY M R, ANTHONY P, POWER J B, et al. Plant protoplasts: status and biotechnological perspectives[J]. Biotechnology Advances, 2005, 23(2): 131−171.
[11] MA Wenjun, YI Fei, XIAO Yao, et al. Isolation of leaf mesophyll protoplasts optimized by orthogonal design for transient gene expression in Catalpa bungei[J/OL]. Scientia Horticulturae, 2020, 274: 109684[2024-09-30]. DOI: 10.1016/j.scienta.2020.109684.
[12] 史勇, 金维环, 刘姣姣, 等. 一种改良的拟南芥原生质体的制备和转化方法[J]. 生物技术, 2019, 29(2): 147−152,170.

SHI Yong, JIN Weihuan, LIU Jiaojiao, et al. An improved method for Arabidopsis mesophyll protoplast isolation and transformation[J]. Biotechnology, 2019, 29(2): 147−152, 170.
[13] 肖政, 徐艳琴, 罗念, 等. 植物原生质体在分子细胞生物学研究中的应用[J]. 广西植物, 2020, 40(4): 576−582.

XIAO Zheng, XU Yanqin, LUO Nian, et al. Application of plant protoplasts in molecular and cell biology research[J]. Guihaia, 2020, 40(4): 576−582.
[14] YANG Chengjun, YU Ruiqiang, LI Jinbo, et al. Preparation of leaf protoplasts from Populus (Populus × xiaohei T. S. Hwang et Liang) and establishment of transient expression system[J/OL]. Journal of Plant Physiology, 2023, 291: 154122[2024-09-30]. DOI: 10.1016/j.jplph.2023.154122.
[15] 叶晶晶, 赵东, 梁月荣, 等. 茶树原生质体制备体系的研究进展[J]. 茶叶, 2021, 47(2): 75−79.

YE Jingjing, ZHAO Dong, LIANG Yuerong, et al. Research progress on protoplast preparation of tea plants[J]. Journal of Tea, 2021, 47(2): 75−79.
[16] 张娅, 刘晓烽, 张婧, 等. 茉莉花原生质体瞬时表达体系的建立及应用[J]. 福建农林大学学报(自然科学版), 2019, 48(6): 727−735.

ZHANG Ya, LIU Xiaofeng, ZHANG Jing, et al. Development of a protoplast-based transient expression system and application in Jasminum sambac[J]. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2019, 48(6): 727−735.
[17] 何珊珊, 李宏宇, 马月, 等. 百合原生质体分离培养和瞬时转化[J]. 浙江大学学报(农业与生命科学版), 2025, 51(1): 67−79.

HE Shanshan, LI Hongyu, MA Yue, et al. Isolation, cultiivation, and transient transformation of lily protoplasts[J]. Journal of Zhejiang University (Agriculture & Life Sciences), 2025, 51(1): 67−79.
[18] 景艳春, 康向阳, 王君, 等. 新疆杨叶肉原生质体游离和纯化的研究[J]. 西北植物学报, 2007, 27(3): 509−514.

JING Yanchun, KANG Xiangyang, WANG Jun, et al. Isolation and purification of mesophyll protoplasts of Populus alba L. var. pyramidalis[J]. Acta Botanica Boreali-Occidentalia Sinica, 2007, 27(3): 509−514.
[19] 彭章, 童华荣, 梁国鲁, 等. 茶树叶片和胚根原生质体的分离及PEG诱导融合[J]. 作物学报, 2018, 44(3): 463−470.

PENG Zhang, TONG Huarong, LIANG Guolu, et al. Protoplast isolation and fusion induced by PEG with leaves and roots of tea plant (Camellia sinensis L. O. kuntze)[J]. Acta Agronomica Sinica, 2018, 44(3): 463−470.
[20] AOYAGI H. Development of a quantitative method for determination of the optimal conditions for protoplast isolation from cultured plant cells[J]. Biotechnology Letters, 2006, 28(20): 1687−1694.
[21] 李婧瑶, 刘龙飚, 丁兵, 等. 植物原生质体分离及培养研究进展[J]. 分子植物育种, 2023, 21(2): 620−632.

LI Jingyao, LIU Longbiao, DING Bing, et al. Research progress on isolation and culture of plant protoplasts[J]. Molecular Plant Breeding, 2023, 21(2): 620−632.
[22] 唐佳妮, 林二培, 黄华宏, 等. 杉木叶片原生质体分离及RNA提取体系的建立[J]. 林业科学, 2018, 54(4): 38−48.

TANG Jiani, LIN Erpei, HUANG Huahong, et al. Isolation and total RNA extraction of leaf protoplasts in Chinese fir[J]. Scientia Silvae Sinicae, 2018, 54(4): 38−48.
[23] HUO Ailing, CHEN Zhenyu, WANG Pengkai, et al. Establishment of transient gene expression systems in protoplasts from Liriodendron hybrid mesophyll cells[J/OL]. PLoS One, 2017, 12(3): e0172475[2024-09-30]. DOI: 10.1371/journal.pone.0172475.
[24] REN Rui, GAO Jie, LU Chuqiao, et al. Highly efficient protoplast isolation and transient expression system for functional characterization of flowering related genes in Cymbidium orchids[J/OL]. International Journal of Molecular Sciences, 2020, 21(7): 2264[2024-09-30]. DOI: 10.3390/ijms21072264.
[25] 曹春艳, 王威, 杨新奇, 等. 槟榔原生质体分离及瞬时转化体系的建立[J]. 分子植物育种, 2023, 21(17): 5730−5737.

CAO Chunyan, WANG Wei, YANG Xinqi, et al. Isolation of protoplast and establishment of transient expression system in Areca catechu[J]. Molecular Plant Breeding, 2023, 21(17): 5730−5737.
[26] KLIMEK-CHODACKA M, KADLUCZKA D, LUKASIEWICZ A, et al. Effective callus induction and plant regeneration in callus and protoplast cultures of Nigella damascena L.[J]. Plant Cell, Tissue and Organ Culture, 2020, 143(3): 693−707.
[27] DU Li, BAO Manzhu. Plant regeneration from protoplasts isolated from embryogenic suspension cultured cells of Cinnamomum camphora L. [J]. Plant Cell Reports, 2005, 24(8): 462−467.
[28] 王一菲, 刘新星, 张青, 等. 油棕叶肉原生质体分离及瞬时转化体系的建立[J]. 华中农业大学学报, 2021, 40(1): 154−159.

WANG Yifei, LIU Xinxing, ZHANG Qing, et al. Isolation of oil palm mesophyll protoplasts and establishment of transient transformation system[J]. Journal of Huazhong Agricultural University, 2021, 40(1): 154−159.
[29] 张天. 国槐叶肉细胞原生质体分离研究[D]. 杨凌: 西北农林科技大学, 2019.

ZHANG Tian. Study on Isolation of Protoplasts from Mesophyll Cells of Sophora japonica[D]. Yangling: Northwest A&F University, 2019.
[30] BAI Liang, CHENG Yan, SHE Jikai, et al. Development of an efficient protoplast isolation and transfection system for Castor bean (Ricinus communis L. )[J]. Plant Cell, Tissue and Organ Culture, 2020, 143(2): 457−464.
[31] HUANG Hongyu, WANG Zhenyu, CHENG Jintao, et al. An efficient cucumber (Cucumis sativus L. ) protoplast isolation and transient expression system[J]. Scientia Horticulturae, 2013, 150: 206−212.
[32] 赖叶林, 贺莹, 李欣欣, 等. 一种植物原生质体分离与瞬时转化的方法[J]. 植物生理学报, 2020, 56(4): 895−903.

LAI Yelin, HE Ying, LI Xinxin, et al. An approach to isolation and transient transformation of protoplasts in plants[J]. Plant Physiology Journal, 2020, 56(4): 895−903.
[33] 谢鑫, 蒋君梅, 王勇, 等. 高粱原生质体的制备及转化方法研究[J]. 种子, 2019, 38(8): 43−46.

XIE Xin, JIANG Junmei, WANG Yong, et al. Study on the method of protoplast isolation and transformation of Sorghum bicolor[J]. Seed, 2019, 38(8): 43−46.
[34] 李青, 鱼海鹏, 张子豪, 等. 棉花真叶原生质体分离及瞬时表达体系的优化[J]. 中国农业科学, 2021, 54(21): 4514−4524.

LI Qing, YU Haipeng, ZHANG Zihao, et al. Optimization of cotton mesophyll protoplast transient expression system[J]. Scientia Agricultura Sinica, 2021, 54(21): 4514−4524.
[35] 李志美, 张碧佩, 伍青, 等. 菊花花瓣原生质体分离与瞬时转化体系的建立[J]. 植物生理学报, 2023, 59(10): 1951−1963.

LI Zhimei, ZHANG Bipei, WU Qing, et al. Establishment of isolation and transient expression system for protoplasts from chrysanthemum petals[J]. Plant Physiology Journal, 2023, 59(10): 1951−1963.