象鼻兰茎尖小液滴玻璃化超低温保存体系建立

赵月; 单璐; 陈之光; 杨树华; 赵鑫; 寇亚平; 葛红; 陈陆琴; 任保青; 郝瑞杰; 贾瑞冬; 赵月; 单璐; 陈之光; 杨树华; 赵鑫; 寇亚平; 葛红; 陈陆琴; 任保青; 郝瑞杰; 贾瑞冬

doi:10.11833/j.issn.2095-0756.20250172

[1]

中国科学院中国植物志编辑委员会. 中国植物志: 第6卷第1分册蕨类植物门[M]. 北京: 科学出版社, 1999: 383.

Editorial Committee of Flora of China, Chinese Academy of Science. Flora of China: Volume 6 Issue 1 Pteridophyta [M]. Beijing: Science Press, 1999: 383.

[2]

阮积惠, 丁炳扬. 象鼻兰[J]. 植物杂志, 1999(2): 8.

RUAN Jihui, DING Bingyang. Phalaenopsis zhejiangensis [J]. Plant Magazine, 1999(2): 8.

[3]

JIANG Ming, ZHU Yan, WU Qian, et al. Complete chloroplast genome of a rare and endangered plant species Phalaenopsis zhejiangensis: genomic features and phylogenetic relationship within Orchidaceae [J]. Mitochondrial DNA Part B, 2021, 6(10): 2872−2879.

[4]

吴婷, 葛红, 杨树华, 等. 秋水仙素诱导象鼻兰种子产生多倍体[J]. 北方园艺, 2022(2): 71−78.

WU Ting, GE Hong, YANG Shuhua, et al. Colchicine induces polyploidy in the seeds of Phalaenopsis zhejiangensis [J]. Northern Horticulture, 2022(2): 71−78.

[5]

欧阳英. 几种兰科花卉的离体保存技术研究[D]. 北京: 北京林业大学, 2010.

OUYANG Ying. Study on in vitro Preservation Technology of Several Orchidaceae Flowers[D]. Beijing: Beijing Forestry University, 2010.

[6]

边佳琪. 草莓组培苗种质资源离体保存的研究[D]. 天津: 天津农学院, 2020.

BIAN Jiaqi. Study on in vitro Conservation of Strawberry Tissue Culture Seedling Germplasm Resources[D]. Tianjin: Tianjin Agricultural University, 2020.

[7]

王逸涵, 王烁凯, 董美响, 等. 果树种质资源保存研究进展[J]. 北方园艺, 2024(5): 127−131.

WANG Yihan, WANG Shuokai, DONG Meixiang, et al. Progress research on conservation of fruit germplasm resources [J]. Northern Horticulture, 2024(5): 127−131.

[8]

穆玉珍, 朱美瑶. 兰花组织培养研究进展[J]. 现代园艺, 2021(1): 24−26.

MU Yuzhen, ZHU Meiyao. Research progress in tissue culture of orchids [J]. Xiandai Horticulture, 2021(1): 24−26.

[9]

LI Jingwei, ZHANG Xiaochen, WANG Minrui, et al. Development, progress and future prospects in cryobiotechnology of Lilium spp. [J/OL]. Plant Methods, 2019, 15(1): 125[2025-02-25]. DOI: 10.1186/s13007-019-0506-9.

[10]

王君晖, 黄纯农. 玻璃化法──园艺作物茎尖和分生组织超低温保存的新途径: 文献综述[J]. 园艺学报, 1994, 21(3): 277−282.

WANG Junhui, HUANG Chunnong. Vitrification: a new approach for cryopreserving shoot-tips and meristems of horticultural crops [J]. Acta Horticulturae Sinica, 1994, 21(3): 277−282.

[11]

姚昕, 兰芹英, 李枝林. 兰科植物种子及圆球茎的超低温保存研究进展[J]. 云南农业大学学报(自然科学), 2013, 28(6): 891−895.

YAO Xin, LAN Qinying, LI Zhilin. Advances of cryopreservation of orchid seed and protocorm [J]. Journal of Yunnan Agricultural University (Natural Science), 2013, 28(6): 891−895.

[12]

王春浩. 文心兰类原球茎玻璃化超低温保存及相关生理生化与组织学研究[D]. 南京: 南京农业大学, 2015.

WANG Chunhao. The Cryopresevation of Oncidium Protocorm Like Bodies by Vitrification and Their Physiological and Histological Characteristics Analysis[D]. Nanjing: Nanjing Agricultural University, 2015.

[13]

欧阳英, 李秉玲, 刘燕. 密花石斛花粉的保存方法研究[J]. 北京林业大学学报, 2010, 32(6): 151−154.

OUYANG Ying, LI Bingling, LIU Yan. Preservation of Dendrobium densiflorum pollen [J]. Journal of Beijing Forestry University, 2010, 32(6): 151−154.

[14]

陈品良. 植物组织培养物的超低温保存[J]. 武汉植物学研究, 1989, 7(4): 390−398.

CHEN Pinliang. Cryopreservation of plant tissue cultures [J]. Journal of Wuhan Botanical Research, 1989, 7(4): 390−398.

[15]

BAE J, LEE S Y, SONG J Y, et al. Efficient cryopreservation of in vitro grown shoot tips of strawberry (Fragaria × ananassa Duch. ) germplasm using droplet-vitrification [J]. Korean Journal of Plant Resources, 2021, 34(6): 600−607.

[16]

RAHMAH S, AHMAD MUBBARAKH S, SINNIAH U R, et al. Effects of droplet-vitrification on brassidium shooting star’s orchid protocorm-like bodies (PLBs) [J]. Scientia Horticulturae, 2015, 197: 254−260.

[17]

ZARGAR AZAD M, KAVIANI B, SEDAGHATHOOR S. Cold storage and cryopreservation by encapsulation-dehydration of Cephalanthera rubra (L. ) Rich. , a threatened orchid species[J/OL]. Acta Agrobotanica, 2024, 77: 189312[2025-02-24]. DOI: 10.5586/aa/189312.

[18]

PING K S, POOBATHY R R, ZAKARIA R, et al. Development of a PVS2 droplet-vitrification cryopreservation technique for Aranda Broga Blue orchid protocorm-like bodies (PLBs) [J]. Cryo Letters, 2017, 38(4): 290−298.

[19]

彭颖, 陈友锋, 乔谦, 等. 红丽海棠茎尖超低温保存技术研究[J]. 福建农业学报, 2023, 38(5): 566−573.

PENG Ying, CHEN Youfeng, QIAO Qian, et al. Cryopreservation of Malus red splendor crabapple shoots [J]. Fujian Journal of Agricultural Sciences, 2023, 38(5): 566−573.

[20]

BI Wenlu, HAO Xinyi, CUI Zhenhua, et al. Droplet-vitrification cryopreservation of in vitro-grown shoot tips of grapevine (Vitis spp. ) [J]. In Vitro Cellular & Developmental Biology-Plant, 2018, 54(6): 590−599.

[21]

FOLGADO R, PANIS B. Cryopreservation of Ashe magnolia shoot-tips by droplet-vitrification [J]. Acta Horticulturae, 2019, 1234: 233−240.

[22]

WANG Renrui, GAO Xiaoxia, CHEN Long, et al. Shoot recovery and genetic integrity of Chrysanthemum morifolium shoot tips following cryopreservation by droplet-vitrification [J]. Scientia Horticulturae, 2014, 176: 330−339.

[23]

杨晶文, 毛宇金, 李娴, 等. 红豆越橘组培苗茎尖小滴玻璃化超低温保存[J]. 吉林农业大学学报, 2024, 46(3): 439−445.

YANG Jingwen, MAO Yujin, LI Xian, et al. Cryopreservation of shoot tips of lingonberry in vitro culture by droplet-vitrification [J]. Journal of Jilin Agricultural University, 2024, 46(3): 439−445.

[24]

ZHU D Z, CHEN X, WANG J W, et al. Orthogonal test design for optimization of the cryopreservation of Chinese cherry (Prunus pseudocerasus L. ) shoot tips by vitrification [J]. Acta Horticulturae, 2019, 1234: 149−156.

[25]

FENG C H, LI B Q, HU L Y, et al. A comparison of two cryogenic protocols in cryopreservation of apple shoot tips [J]. Acta Horticulturae, 2014, 1039: 161−166.

[26]

O’BRIEN C, HITI-BANDARALAGE J C A, FOLGADO R, et al. First report on cryopreservation of mature shoot tips of two avocado (Persea americana Mill. ) rootstocks [J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2021, 144(1): 103−113.

[27]

WANG M R, ZHANG Z, HAUGSLIEN S, et al. Cryopreservation of shallot (Allium cepa var. aggregatum) shoot tips by droplet-vitrification [J]. Acta Horticulturae, 2019, 1234: 241−248.

[28]

俞嘉卿, 邱涵, 余文捡, 等. 南方红豆杉悬浮细胞超低温保存体系的建立[J]. 江西农业大学学报, 2024, 46(1): 118−127.

YU Jiaqing, QIU Han, YU Wenjian, et al. Establishment of cryopreservation system of Taxus wallichiana var. mairei suspension cells [J]. Acta Agriculturae Universitatis Jiangxiensis, 2024, 46(1): 118−127.

[29]

LURSWIJIDJARUSA W, THAMMASIRI K. Cryopreservation of shoot tips of Dendrobium walter oumae by encapsulation/dehydration [J]. ScienceAsia, 2004, 30: 293−299.

[30]

LIN Liang, MA Junchao, AI Qin, et al. Lipid remodeling confers osmotic stress tolerance to embryogenic cells during cryopreservation[J/OL]. International Journal of Molecular Sciences, 2021, 22(4): 2174[2025-02-24]. DOI: 10.3390/ijms22042174.

[31]

WANG Liyu, LI Yadong, SUN Haiyue, et al. An efficient droplet-vitrification cryopreservation for valuable blueberry germplasm [J]. Scientia Horticulturae, 2017, 219: 60−69.

[32]

HALMAGVI A, PINKER I. Cryopreservation of Rosa shoot tips: importance of preculture conditions [J]. Acta Horticulturae, 2006, 725: 351−356.

[33]

CHEN X L, LI J H, XIN X, et al. Cryopreservation of in vitro-grown apical meristems of Lilium by droplet-vitrification [J]. South African Journal of Botany, 2011, 77(2): 397−403.

[34]

YIN Minghua, HONG Senrong. Cryopreservation of Dendrobium candidum Wall. ex Lindl. protocorm-like bodies by encapsulation-vitrification [J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2009, 98(2): 179−185.

[1]	中国科学院中国植物志编辑委员会. 中国植物志: 第6卷第1分册蕨类植物门[M]. 北京: 科学出版社, 1999: 383. Editorial Committee of Flora of China, Chinese Academy of Science. Flora of China: Volume 6 Issue 1 Pteridophyta [M]. Beijing: Science Press, 1999: 383.
[2]	阮积惠, 丁炳扬. 象鼻兰[J]. 植物杂志, 1999(2): 8. RUAN Jihui, DING Bingyang. Phalaenopsis zhejiangensis [J]. Plant Magazine, 1999(2): 8.
[3]	JIANG Ming, ZHU Yan, WU Qian, et al. Complete chloroplast genome of a rare and endangered plant species Phalaenopsis zhejiangensis: genomic features and phylogenetic relationship within Orchidaceae [J]. Mitochondrial DNA Part B, 2021, 6(10): 2872−2879.
[4]	吴婷, 葛红, 杨树华, 等. 秋水仙素诱导象鼻兰种子产生多倍体[J]. 北方园艺, 2022(2): 71−78. WU Ting, GE Hong, YANG Shuhua, et al. Colchicine induces polyploidy in the seeds of Phalaenopsis zhejiangensis [J]. Northern Horticulture, 2022(2): 71−78.
[5]	欧阳英. 几种兰科花卉的离体保存技术研究[D]. 北京: 北京林业大学, 2010. OUYANG Ying. Study on in vitro Preservation Technology of Several Orchidaceae Flowers[D]. Beijing: Beijing Forestry University, 2010.
[6]	边佳琪. 草莓组培苗种质资源离体保存的研究[D]. 天津: 天津农学院, 2020. BIAN Jiaqi. Study on in vitro Conservation of Strawberry Tissue Culture Seedling Germplasm Resources[D]. Tianjin: Tianjin Agricultural University, 2020.
[7]	王逸涵, 王烁凯, 董美响, 等. 果树种质资源保存研究进展[J]. 北方园艺, 2024(5): 127−131. WANG Yihan, WANG Shuokai, DONG Meixiang, et al. Progress research on conservation of fruit germplasm resources [J]. Northern Horticulture, 2024(5): 127−131.
[8]	穆玉珍, 朱美瑶. 兰花组织培养研究进展[J]. 现代园艺, 2021(1): 24−26. MU Yuzhen, ZHU Meiyao. Research progress in tissue culture of orchids [J]. Xiandai Horticulture, 2021(1): 24−26.
[9]	LI Jingwei, ZHANG Xiaochen, WANG Minrui, et al. Development, progress and future prospects in cryobiotechnology of Lilium spp. [J/OL]. Plant Methods, 2019, 15(1): 125[2025-02-25]. DOI: 10.1186/s13007-019-0506-9.
[10]	王君晖, 黄纯农. 玻璃化法──园艺作物茎尖和分生组织超低温保存的新途径: 文献综述[J]. 园艺学报, 1994, 21(3): 277−282. WANG Junhui, HUANG Chunnong. Vitrification: a new approach for cryopreserving shoot-tips and meristems of horticultural crops [J]. Acta Horticulturae Sinica, 1994, 21(3): 277−282.
[11]	姚昕, 兰芹英, 李枝林. 兰科植物种子及圆球茎的超低温保存研究进展[J]. 云南农业大学学报(自然科学), 2013, 28(6): 891−895. YAO Xin, LAN Qinying, LI Zhilin. Advances of cryopreservation of orchid seed and protocorm [J]. Journal of Yunnan Agricultural University (Natural Science), 2013, 28(6): 891−895.
[12]	王春浩. 文心兰类原球茎玻璃化超低温保存及相关生理生化与组织学研究[D]. 南京: 南京农业大学, 2015. WANG Chunhao. The Cryopresevation of Oncidium Protocorm Like Bodies by Vitrification and Their Physiological and Histological Characteristics Analysis[D]. Nanjing: Nanjing Agricultural University, 2015.
[13]	欧阳英, 李秉玲, 刘燕. 密花石斛花粉的保存方法研究[J]. 北京林业大学学报, 2010, 32(6): 151−154. OUYANG Ying, LI Bingling, LIU Yan. Preservation of Dendrobium densiflorum pollen [J]. Journal of Beijing Forestry University, 2010, 32(6): 151−154.
[14]	陈品良. 植物组织培养物的超低温保存[J]. 武汉植物学研究, 1989, 7(4): 390−398. CHEN Pinliang. Cryopreservation of plant tissue cultures [J]. Journal of Wuhan Botanical Research, 1989, 7(4): 390−398.
[15]	BAE J, LEE S Y, SONG J Y, et al. Efficient cryopreservation of in vitro grown shoot tips of strawberry (Fragaria × ananassa Duch. ) germplasm using droplet-vitrification [J]. Korean Journal of Plant Resources, 2021, 34(6): 600−607.
[16]	RAHMAH S, AHMAD MUBBARAKH S, SINNIAH U R, et al. Effects of droplet-vitrification on brassidium shooting star’s orchid protocorm-like bodies (PLBs) [J]. Scientia Horticulturae, 2015, 197: 254−260.
[17]	ZARGAR AZAD M, KAVIANI B, SEDAGHATHOOR S. Cold storage and cryopreservation by encapsulation-dehydration of Cephalanthera rubra (L. ) Rich. , a threatened orchid species[J/OL]. Acta Agrobotanica, 2024, 77: 189312[2025-02-24]. DOI: 10.5586/aa/189312.
[18]	PING K S, POOBATHY R R, ZAKARIA R, et al. Development of a PVS2 droplet-vitrification cryopreservation technique for Aranda Broga Blue orchid protocorm-like bodies (PLBs) [J]. Cryo Letters, 2017, 38(4): 290−298.
[19]	彭颖, 陈友锋, 乔谦, 等. 红丽海棠茎尖超低温保存技术研究[J]. 福建农业学报, 2023, 38(5): 566−573. PENG Ying, CHEN Youfeng, QIAO Qian, et al. Cryopreservation of Malus red splendor crabapple shoots [J]. Fujian Journal of Agricultural Sciences, 2023, 38(5): 566−573.
[20]	BI Wenlu, HAO Xinyi, CUI Zhenhua, et al. Droplet-vitrification cryopreservation of in vitro-grown shoot tips of grapevine (Vitis spp. ) [J]. In Vitro Cellular & Developmental Biology-Plant, 2018, 54(6): 590−599.
[21]	FOLGADO R, PANIS B. Cryopreservation of Ashe magnolia shoot-tips by droplet-vitrification [J]. Acta Horticulturae, 2019, 1234: 233−240.
[22]	WANG Renrui, GAO Xiaoxia, CHEN Long, et al. Shoot recovery and genetic integrity of Chrysanthemum morifolium shoot tips following cryopreservation by droplet-vitrification [J]. Scientia Horticulturae, 2014, 176: 330−339.
[23]	杨晶文, 毛宇金, 李娴, 等. 红豆越橘组培苗茎尖小滴玻璃化超低温保存[J]. 吉林农业大学学报, 2024, 46(3): 439−445. YANG Jingwen, MAO Yujin, LI Xian, et al. Cryopreservation of shoot tips of lingonberry in vitro culture by droplet-vitrification [J]. Journal of Jilin Agricultural University, 2024, 46(3): 439−445.
[24]	ZHU D Z, CHEN X, WANG J W, et al. Orthogonal test design for optimization of the cryopreservation of Chinese cherry (Prunus pseudocerasus L. ) shoot tips by vitrification [J]. Acta Horticulturae, 2019, 1234: 149−156.
[25]	FENG C H, LI B Q, HU L Y, et al. A comparison of two cryogenic protocols in cryopreservation of apple shoot tips [J]. Acta Horticulturae, 2014, 1039: 161−166.
[26]	O’BRIEN C, HITI-BANDARALAGE J C A, FOLGADO R, et al. First report on cryopreservation of mature shoot tips of two avocado (Persea americana Mill. ) rootstocks [J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2021, 144(1): 103−113.
[27]	WANG M R, ZHANG Z, HAUGSLIEN S, et al. Cryopreservation of shallot (Allium cepa var. aggregatum) shoot tips by droplet-vitrification [J]. Acta Horticulturae, 2019, 1234: 241−248.
[28]	俞嘉卿, 邱涵, 余文捡, 等. 南方红豆杉悬浮细胞超低温保存体系的建立[J]. 江西农业大学学报, 2024, 46(1): 118−127. YU Jiaqing, QIU Han, YU Wenjian, et al. Establishment of cryopreservation system of Taxus wallichiana var. mairei suspension cells [J]. Acta Agriculturae Universitatis Jiangxiensis, 2024, 46(1): 118−127.
[29]	LURSWIJIDJARUSA W, THAMMASIRI K. Cryopreservation of shoot tips of Dendrobium walter oumae by encapsulation/dehydration [J]. ScienceAsia, 2004, 30: 293−299.
[30]	LIN Liang, MA Junchao, AI Qin, et al. Lipid remodeling confers osmotic stress tolerance to embryogenic cells during cryopreservation[J/OL]. International Journal of Molecular Sciences, 2021, 22(4): 2174[2025-02-24]. DOI: 10.3390/ijms22042174.
[31]	WANG Liyu, LI Yadong, SUN Haiyue, et al. An efficient droplet-vitrification cryopreservation for valuable blueberry germplasm [J]. Scientia Horticulturae, 2017, 219: 60−69.
[32]	HALMAGVI A, PINKER I. Cryopreservation of Rosa shoot tips: importance of preculture conditions [J]. Acta Horticulturae, 2006, 725: 351−356.
[33]	CHEN X L, LI J H, XIN X, et al. Cryopreservation of in vitro-grown apical meristems of Lilium by droplet-vitrification [J]. South African Journal of Botany, 2011, 77(2): 397−403.
[34]	YIN Minghua, HONG Senrong. Cryopreservation of Dendrobium candidum Wall. ex Lindl. protocorm-like bodies by encapsulation-vitrification [J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2009, 98(2): 179−185.