[1] ATHENSTAEDT K, DAUM G. Phosphatidic acid, a key intermediate in lipid metabolism [J]. European Journal of Biochemistry, 1999, 266(1): 1 − 16.
[2] MURATA N, TASAKA Y. Glycerol-3-phosphate acyltransferase in plants [J]. Biochimica et Biophysica Acta, 1997, 1348(1/2): 10 − 16.
[3] GIMENO R E, CAO Jingsong. Thematic review series: glycerolipids. mammalian glycerol-3-phosphate acyltransferases: new genes for an old activity [J]. Journal of Lipid Research, 2008, 49(10): 2079 − 2088.
[4] WENDEL A A, LEWIN T M, COLEMAN R A. Glycerol-3-phosphate acyltransferases: rate limiting enzymes of triacylglycerol biosynthesis [J]. Biochimica et Biophysica Acta, 2009, 1791(6): 501 − 506.
[5] ZHENG Zhifu, ZOU Jitao. The initial step of the glycerolipid pathway: identification of glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate acyltransferases in Saccharomyces cerevisiae [J]. The Journal of Biological Chemistry, 2001, 276(45): 41710 − 41716.
[6] ZHENG Zhifu, XIA Qun, DAUK M, et al. Arabidopsis AtGPAT1, a member of the membrane-bound glycerol-3-phosphate acyltransferase gene family, is essential for tapetum differentiation and male fertility [J]. The Plant Cell, 2003, 15(8): 1872 − 1887.
[7] ZOU Jitao, WEI Yangdou, JAKO C, et al. The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene [J]. The Plant Journal, 1999, 19(6): 645 − 653.
[8] DAHLQVIST A, STAHL U, LENMAN M, et al. Phospholipid: diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants [J]. Proceedings of the National Academy of Sciences of the United States of America, 2000, 97(12): 6487 − 6492.
[9] STÅHL U, CARLSSON A S, LENMAN M, et al. Cloning and functional characterization of a phospholipid: diacylglycerol acyltransferase from Arabidopsis [J]. Plant Physiology, 2004, 135(3): 1324 − 1335.
[10] ZHANG Meng, FAN Jilian, TAYLOR D C, et al. DGAT1 and PDAT1 acyltransferases have overlapping functions in Arabidopsis triacylglycerol biosynthesis and are essential for normal pollen and seed development [J]. The Plant Cell, 2009, 21(12): 3885 − 3901.
[11] WYDYSH E A, MEDGHALCHI S M, VADLAMUDI A, et al. Design and synthesis of small molecule glycerol 3-phosphate acyltransferase inhibitors [J]. Journal of Medicinal Chemistry, 2009, 52(10): 3317 − 3327.
[12] WYDYSH E A, VADLAMUDI A, MEDGHALCHI S M, et al. Design, synthesis, and biological evaluation of conformationally constrained glycerol 3-phosphate acyltransferase inhibitors [J]. Bioorganic &Medicinal Chemistry, 2010, 18(17): 6470 − 6479.
[13] ELLIS J M, PAUL D S, DEPETRILLO M A, et al. Mice deficient in glycerol-3-phosphate acyltransferase-1 have a reduced susceptibility to liver cancer [J]. Toxicologic Pathology, 2012, 40(3): 513 − 521.
[14] CAO Jingsong, PEREZ S, GOODWIN B, et al. Mice deleted for GPAT3 have reduced GPAT activity in white adipose tissue and altered energy and cholesterol homeostasis in diet-induced obesity [J/OL]. American Journal of Physiology. Endocrinology and Metabolism, 2014, 306(10): E1176-1187[2022-12-12]. doi: 10.1152/ajpendo.00666.2013.
[15] OUTLAW V K, WYDYSH E A, VADLAMUDI A, et al. Design, synthesis, and evaluation of 4- and 5-substituted o-(octanesulfonamido)benzoic acids as inhibitors of glycerol-3-phosphate acyltransferase [J]. Medchemcomm, 2014, 5(6): 826 − 830.
[16] PELLON-MAISON M, MONTANARO M A, LACUNZA E, et al. Glycerol-3-phosphate acyltranferase-2 behaves as a cancer testis gene and promotes growth and tumorigenicity of the breast cancer MDA-MB-231 cell line [J/OL]. PLoS One, 2014, 9(6): e100896[2022-12-12]. doi: 10.1371/journal.pone.0100896.
[17] TURNBULL A P, RAFFERTY J B, SEDELNIKOVA S E, et al. Analysis of the structure, substrate specificity, and mechanism of squash glycerol-3-phosphate (1)-acyltransferase [J]. Structure, 2001, 9(5): 347 − 353.
[18] LEWIN T M, WANG Ping, COLEMAN R A. Analysis of amino acid motifs diagnostic for the sn-glycerol-3-phosphate acyltransferase reaction [J]. Biochemistry, 1999, 38(18): 5764 − 5771.
[19] YANG S U, KIM J, KIM H, et al. Functional characterization of Physcomitrellapatens glycerol-3-phosphate acyltransferase 9 and an increase in seed oil content in arabidopsis by its ectopic expression [J/OL]. Plants, 2019, 8(8): 284[2022-12-12]. doi: 10.3390/plants8080284.
[20] GIDDA S K, SHOCKEY J M, ROTHSTEIN S J, et al. Arabidopsis thaliana GPAT8 and GPAT9 are localized to the ER and possess distinct ER retrieval signals: functional divergence of the dilysine ER retrieval motif in plant cells [J]. Plant Physiology and Biochemistry, 2009, 47(10): 867 − 879.
[21] SHOCKEY J, REGMI A, COTTON K, et al. Identification of Arabidopsis GPAT9 (At5g60620) as an essential gene involved in triacylglycerol biosynthesis [J]. Plant Physiology, 2016, 170(1): 163 − 179.
[22] SINGER S D, CHEN Guanqun, MIETKIEWSKA E, et al. Arabidopsis GPAT9 contributes to synthesis of intracellular glycerolipids but not surface lipids [J]. Journal of Experimental Botany, 2016, 67(15): 4627 − 4638.
[23] 陈丹丹. 拟南芥3-磷酸甘油酰基转移酶2/3/9的结构与功能分析[D]. 杭州: 浙江农林大学, 2019.

CHEN Dandan. Structure and Functional Analysis of Arabidopsis thaliana Glycerol-3-phosphate Acyltransferase 2/3/9 [D]. Hangzhou: Zhejiang A&F University, 2019.
[24] 段芊芊, 林怡馨, 丁硕, 等. 13个甘蓝型油菜GPATs编码基因的酵母遗传互补功能鉴定[J]. 农业生物技术学报, 2020, 28(7): 1156 − 1164.

DUAN Qianqian, LIN Yixin, DING Shuo, et al. Functional identification of 13 Brassica napus GPATs encoding genes by genetic complementation in yeast (Saccharomyces cerevisiae) [J]. Journal of Agricultural Biotechnology, 2020, 28(7): 1156 − 1164.
[25] LEI Jie, MIAO Yingchun, LAN Yu, et al. A novel complementation assay for quick and specific screen of genes encoding glycerol-3-phosphate acyltransferases [J/OL]. Frontiers in Plant Science, 2018, 9: 353[2022-12-12]. doi: 10.3389/fpls.2018.00353.
[26] WASCHBURGER E, KULCHESKI F R, VETO N M, et al. Genome-wide analysis of the glycerol-3-phosphate acyltransferase (GPAT) gene family reveals the evolution and diversification of plant GPATs [J]. Genetics and Molecular Biology, 2018, 41(suppl 1): 355−370.
[27] 邢蔓, 周雪晴, 何婷, 等. 甘蓝型油菜BnGPAT9基因表达模式及其苗期非生物胁迫表达分析[J]. 中国油料作物学报, 2017, 39(4): 454 − 461.

XING Man, ZHOU Xueqing, HE Ting, et al. Expression pattern of BnGPAT9 gene in Brassica napus and its expression under abiotic stresses [J]. Chinese Journal of Oil Crop Sciences, 2017, 39(4): 454 − 461.
[28] KROGH A, LARSSON B, von HEIJINE G, et al. Predicting transmembrane protein topology with a hidden markov model: application to complete genomes [J]. Journal of Molecular Biology, 2001, 305(3): 567 − 580.
[29] OMASITS U, AHRENS C H, MÜLLER S, et al. Protter: interactive protein feature visualization and integration with experimental proteomic data [J]. Bioinformatics, 2014, 30(6): 884 − 886.
[30] YANG Jianxi, YAN Renxiang, ROY A, et al. The I-TASSER Suite: protein structure and function prediction [J]. Nature Methods, 2015, 12(1): 7 − 8.
[31] 陈丹丹, 刘宏波. 筛选GPAT基因的酵母遗传互补体系的优化[J]. 江苏农业科学, 2019, 47(13): 64 − 66.

CHEN Dandan, LIU Hongbo. Optimization of yeast genetic complementary system for screening GPAT genes [J]. Jiangsu Agricultural Sciences, 2019, 47(13): 64 − 66.
[32] SUN Baocheng, GUO Xuejie, FAN Chengming, et al. Newly identified essential amino acids affecting Chlorella ellipsoidea DGAT1 function revealed by site-directed mutagenesis [J/OL]. International Journal of Molecular Sciences, 2018, 19(11): 3462[2022-12-12].doi: 10.3390/ijms19113462.
[33] HEATH R J, ROCK C O. A conserved histidine is essential for glycerolipid acyltransferase catalysis [J]. Journal of Bacteriology, 1998, 180(6): 1425 − 1430.
[34] BRONNIKOV G E, ABOULAICH N, VENER A V, et al. Acute effects of insulin on the activity of mitochondrial GPAT1 in primary adipocytes [J]. Biochemical and Biophysical Research Communications, 2008, 367(1): 201 − 207.
[35] BRATSCHI M W, BURROWES D P, KULAGA A, et al. Glycerol-3-phosphate acyltransferases gat1p and gat2p are microsomal phosphoproteins with differential contributions to polarized cell growth [J]. Eukaryotic Cell, 2009, 8(8): 1184 − 1196.
[36] MITON C M, BUDA K, TOKURIKI N. Epistasis and intramolecular networks in protein evolution [J]. Current Opinion in Structural Biology, 2021, 69: 160 − 168.
[37] HEATH R J, ROCK C O. A missense mutation accounts for the defect in the glycerol-3-phosphate acyltransferase expressed in the plsB26 mutant [J]. Journal of Bacteriology, 1999, 181(6): 1944 − 1946.