Haplocladium microphyllum spore germination with different culture mediums and sucrose concentrations
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摘要: 将细叶小羽藓Haplocladium microphyllum孢子接种在Beneck,1/2MS (Murashige and Skoog),MS,Knop,改良Knop 等5种培养基上,用pH酸度计将酸碱度调至pH 7.0。分别加入适量蔗糖,5种培养基各设置不同蔗糖质量浓度:40,30,20,10,0 gL-1,定时镜检孢子的萌发情况。结果表明:当蔗糖质量浓度为0 ~ 30 gL-1时随蔗糖质量浓度升高孢子萌发率升高,蔗糖质量浓度为30 gL-1时孢子萌发率最高,当蔗糖质量浓度超过40 gL-1时,孢子萌发受抑制。在蔗糖质量浓度为30 gL-1的培养基上培养7 d后,MS培养基上孢子萌发率最高,高达95.93%;Knop培养基上孢子最终萌发率最低,仅为60% ~ 75%。图6参14Abstract: Spores of Haplocladium microphyllum were inoculated on Benecke,1/2MS (Murashige and Skoog),MS,Knop,and improved Benecks culture mediums,and pH was adjusted to 7.0 with a pH meter. Sucrose at 40,30,20,10,and 0 gL-1 was added,and germination of the spores was observed by microscope after 24 h. Results showed that for treatments from 0-30 gL-1 sucrose concentration increased. Germination of the spores was highest with 30 gL-1 sucrose concentration and inhibited with 40 gL-1. After 7 d,the spore germination rate was greatest with the MS medium at 30 gL-1 (at 95.93%) and least with the Knop medium (60%-75%). It indicates the spore germination of Haplocladium microphyllum need certain osmotic pressure,but it would be inhibited in high osmotic pressure caused by high sucrose concentration.[Ch,6 fig. 14 ref.]
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Key words:
- botany /
- Haplocladium microphyllum /
- spore germination /
- culture medium /
- sucrose concentration
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2012.03.009
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