Volume 29 Issue 3
May  2012
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HANG Lu-lu, ZHANG Nan, JI Meng-cheng. Haplocladium microphyllum spore germination with different culture mediums and sucrose concentrations[J]. Journal of Zhejiang A&F University, 2012, 29(3): 383-387. doi: 10.11833/j.issn.2095-0756.2012.03.009
Citation: HANG Lu-lu, ZHANG Nan, JI Meng-cheng. Haplocladium microphyllum spore germination with different culture mediums and sucrose concentrations[J]. Journal of Zhejiang A&F University, 2012, 29(3): 383-387. doi: 10.11833/j.issn.2095-0756.2012.03.009

Haplocladium microphyllum spore germination with different culture mediums and sucrose concentrations

doi: 10.11833/j.issn.2095-0756.2012.03.009
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  • Corresponding author: JI Meng-cheng
  • Received Date: 2011-05-01
  • Rev Recd Date: 2011-09-20
  • Publish Date: 2012-06-20
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Haplocladium microphyllum spore germination with different culture mediums and sucrose concentrations

doi: 10.11833/j.issn.2095-0756.2012.03.009
    Corresponding author: JI Meng-cheng

Abstract: Spores of Haplocladium microphyllum were inoculated on Benecke,1/2MS (Murashige and Skoog),MS,Knop,and improved Benecks culture mediums,and pH was adjusted to 7.0 with a pH meter. Sucrose at 40,30,20,10,and 0 gL-1 was added,and germination of the spores was observed by microscope after 24 h. Results showed that for treatments from 0-30 gL-1 sucrose concentration increased. Germination of the spores was highest with 30 gL-1 sucrose concentration and inhibited with 40 gL-1. After 7 d,the spore germination rate was greatest with the MS medium at 30 gL-1 (at 95.93%) and least with the Knop medium (60%-75%). It indicates the spore germination of Haplocladium microphyllum need certain osmotic pressure,but it would be inhibited in high osmotic pressure caused by high sucrose concentration.[Ch,6 fig. 14 ref.]

HANG Lu-lu, ZHANG Nan, JI Meng-cheng. Haplocladium microphyllum spore germination with different culture mediums and sucrose concentrations[J]. Journal of Zhejiang A&F University, 2012, 29(3): 383-387. doi: 10.11833/j.issn.2095-0756.2012.03.009
Citation: HANG Lu-lu, ZHANG Nan, JI Meng-cheng. Haplocladium microphyllum spore germination with different culture mediums and sucrose concentrations[J]. Journal of Zhejiang A&F University, 2012, 29(3): 383-387. doi: 10.11833/j.issn.2095-0756.2012.03.009

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