外源多胺对银杏悬浮细胞生长及类黄酮合成的影响

鞠定顺; 狄晶晶; 陈颖; 刘瑞; 蔡昊; 鞠定顺; 狄晶晶; 陈颖; 刘瑞; 蔡昊

doi:10.11833/j.issn.2095-0756.20250518

[1]

DZIURKA K, DZIURKA M, CZYCZYŁO-MYSZA I, et al. Insight into primary and secondary metabolites of 21-day-old haploid oat embryos[J]. Journal of Plant Growth Regulation, 2025, 44(8): 4396−4407. DOI: 10.1007/s00344-025-11694-8.

[2]

孙天丽, 黄明珠, 刘斌, 等. 基于合成生物学微生物底盘细胞构建与优化的研究进展[J]. 食品工业科技, 2025, 46(1): 394−402.

SUN Tianli, HUANG Mingzhu, LIU Bin, et al. Research progress in the construction and optimization of microbial chassis cells based on synthetic biology[J]. Science and Technology of Food Industry, 2025, 46(1): 394−402. DOI: 10.13386/j.issn1002-0306.2023120354.

[3]

颜钊涛, 周鹏飞, 汪阳忠, 等. 植物合成生物学: 植物细胞大规模培养的新机遇[J]. 合成生物学, 2025(5): 1107−1125.

YAN Zhaotao, ZHOU Pengfei, WANG Yangzhong, et al. Plant synthetic biology: a new opportunity for large-scale culture of plant cells[J]. Synthetic Biology Journal, 2025(5): 1107−1125. DOI: 10.12211/2096-8280.2024-095.

[4]

ARYA S S, ROOKES J E, CAHILL D M, et al. Next-generation metabolic engineering approaches towards development of plant cell suspension cultures as specialized metabolite producing biofactories[J]. Biotechnology Advances, 2020, 45: 107635. DOI: 10.1016/j.biotechadv.2020.107635.

[5]

王沐兰, 杨生超, 郁步竹, 等. 红豆杉高产悬浮细胞系建立及其紫杉醇诱导的研究进展[J]. 广西植物, 2016, 36(9): 1137−1146.

WANG Mulan, YANG Shengchao, YU Buzhu, et al. Research progress on establishment of high-yield suspension cell line of Taxus chinensis and its taxol induction[J]. Guihaia, 2016, 36(9): 1137−1146. DOI: 10.11931/guihaia.gxzw201412021.

[6]

LIANG Chuxin, CHEN Chang, ZHOU Pengfei, et al. Effect of Aspergillus flavus fungal elicitor on the production of terpenoid indole alkaloids in Catharanthus roseus cambial meristematic cells[J]. Molecules, 2018, 23(12): 3276. DOI: 10.3390/molecules23123276.

[7]

PRASHANT S P, BHAWANA M. An update on biotechnological intervention mediated by plant tissue culture to boost secondary metabolite production in medicinal and aromatic plants[J]. Physiologia Plantarum, 2024, 176(4): e14400. DOI: 10.1111/ppl.14400.

[8]

NANDY S, DAS T, TUDU C K, et al. Unravelling the multi-faceted regulatory role of polyamines in plant biotechnology, transgenics and secondary metabolomics[J]. Applied Microbiology and Biotechnology, 2022, 106(3): 905−929. DOI: 10.1007/s00253-021-11748-3.

[9]

MUSTAFAVI S H, NAGHDI BADI H, SĘKARA A, et al. Polyamines and their possible mechanisms involved in plant physiological processes and elicitation of secondary metabolites[J]. Acta Physiologiae Plantarum, 2018, 40(6): 102. DOI: 10.1007/s11738-018-2671-2.

[10]

CHEN Dandan, SHAO Qingsong, YIN Lianghong, et al. Polyamine function in plants: metabolism, regulation on development, and roles in abiotic stress responses[J]. Frontiers in Plant Science, 2019, 9: 1945. DOI: 10.3389/fpls.2018.01945.

[11]

杨硕知, 刘球, 吴际友, 等. 外源多胺对红椿S-腺苷甲硫氨酸脱羧酶基因表达的调节作用[J]. 中南林业科技大学学报, 2019, 39(1): 116−123.

YANG Shuozhi, LIU Qiu, WU Jiyou, et al. Regulation of exogenous polyamines on S- adenosylmethionine decarboxylase gene expression in Toona sinensis[J]. Journal of Central South University of Forestry & Technology, 2019, 39(1): 116−123. DOI: 10.14067/j.cnki.1673-923x.2019.01.018.

[12]

王果, 刘耀婷, 李焕苓, 等. 多胺对荔枝胚性愈伤组织增殖与体胚发生的影响[J]. 广西植物, 2024, 44(7): 1307−1318.

WANG Guo, LIU Yaoting, LI Huanling, et al. Influences of polyamines on embryogenic callus proliferation and somatic embryogenesis in Litchi chinensis[J]. Guihaia, 2024, 44(7): 1307−1318. DOI: 10.11931/guihaia.gxzw202308066.

[13]

VONDRÁKOVÁ Z, ELIÁŠOVÁ K, VÁGNER M, et al. Exogenous putrescine affects endogenous polyamine levels and the development of Picea abies somatic embryos[J]. Plant Growth Regulation, 2015, 75(2): 405−414. DOI: 10.1007/s10725-014-0001-2.

[14]

BISWAS D, TARUN HALDER T, BISWAJIT GHOSH B. Enhancing secondary metabolite biosynthesis from ethnomedicinal plant Solanum nigrum L. through cytogenetically stable mass propagation, transgenic hairy root induction, and using different LED light and culture vessels for hairy root culture [J]. Journal of Biotechnology, 2025, 404: 63−82.

[15]

BISWAS D, HALDER T, GHOSH B. Enhancing secondary metabolite biosynthesis from ethnomedicinal plant Solanum nigrum L. through cytogenetically stable mass propagation, transgenic hairy root induction, and using different LED light and culture vessels for hairy root culture[J]. Journal of Biotechnology, 2025, 404: 63−82. DOI: 10.1016/j.jbiotec.2025.04.003.

[16]

DEY A, HAZRA A K, NONGDAM P, et al. Enhanced bacoside content in polyamine treated in-vitro raised Bacopa monnieri (L. ) Wettst[J]. South African Journal of Botany, 2019, 123: 259−269. DOI: 10.1016/j.sajb.2019.03.012.

[17]

YASEMIN C, VILDAN Y. Polyamine elicitation of quercetin and rutin production in callus cultures of caper and impact to regeneration[J]. Journal of Scientific and Industrial Research, 2022, 81(5): 475−481. DOI: 10.56042/jsir.v81i05.58826.

[18]

DEY A, NONGDAM P, NANDY S, et al. Polyamine elicited aristolochic acid production in in vitro clonally fidel Aristolochia indica L. : an ISSR and RAPD markers and HPTLC based study[J]. South African Journal of Botany, 2021, 140: 326−335. DOI: 10.1016/j.sajb.2020.06.018.

[19]

HAO Gangping, JI Haiwei, LI Yanlin, et al. Exogenous ABA and polyamines enhanced salvianolic acids contents in hairy root cultures of Salvia miltiorrhiza Bge. F. alba[J]. Plant Omics Journal, 2012, 5(5): 446−452.

[20]

COSKUN Y, YAMAN V. Enhancing phenolic compounds in caper through the incorporation of polyamines in in vitro cultures from various explant sources[J]. South African Journal of Botany, 2024, 165: 43−48. DOI: 10.1016/j.sajb.2023.12.017.

[21]

YU Yao, ZHANG Wanbo, LI Xiying, et al. Pathogenic fungal elicitors enhance ginsenoside biosynthesis of adventitious roots in Panax quinquefolius during bioreactor culture[J]. Industrial Crops and Products, 2016, 94: 729−735. DOI: 10.1016/j.indcrop.2016.09.058.

[22]

CHEN Ying, HU Yuchen, WANG Ruimin, et al. Transcriptome and physiological analysis highlights the hormone, phenylpropanoid, and photosynthesis effects on early somatic embryogenesis in Ginkgo biloba[J]. Industrial Crops and Products, 2023, 203: 117176. DOI: 10.1016/j.indcrop.2023.117176.

[23]

ABDEL SALAM S, GEORGE C S, AL ABDULSALAM N K, et al. Ginkgo biloba attenuates complete Freund’s adjuvant-induced inflammatory pain by suppressing the NF-κB-CXCL1/CXCR2 signaling cascade in the rat spinal cord[J]. Redox Report, 2025, 30(1): 2447778. DOI: 10.1080/13510002.2024.2447778.

[24]

CHEN Ying, LIN Fangzhi, YANG Hua, et al. Effect of varying NaCl doses on flavonoid production in suspension cells of Ginkgo biloba: relationship to chlorophyll fluorescence, ion homeostasis, antioxidant system and ultrastructure[J]. Acta Physiologiae Plantarum, 2014, 36(12): 3173−3187. DOI: 10.1007/s11738-014-1684-8.

[25]

PÉREZ-AMBROCIO A, GUERRERO-BELTRÁN J A, APARICIO-FERNÁNDEZ X, et al. Effect of blue and ultraviolet-C light irradiation on bioactive compounds and antioxidant capacity of Habanero pepper (Capsicum chinense) during refrigeration storage[J]. Postharvest Biology and Technology, 2018, 135: 19−26. DOI: 10.1016/j.postharvbio.2017.08.023.

[26]

张妍妍, 卢玉, 史保争, 等. 植物中多胺的代谢及生理功能研究进展[J]. 北方园艺, 2023(15): 122−127.

ZHANG Yanyan, LU Yu, SHI Baozheng, et al. Research progress on metabolism and physiological function of polyamines in plants[J]. Northern Horticulture, 2023(15): 122−127. DOI: 10.11937/bfyy.20224477.

[27]

杨正宇, 周佳君, 胡恒康, 等. 香榧胚性愈伤组织的悬浮培养动力学及对赤霉素的响应[J]. 浙江农林大学学报, 2025, 42(1): 103−111.

YANG Zhengyu, ZHOU Jiajun, HU Hengkang, et al. Suspension culture dynamics of embryogenic callus from Torreya grandis ‘Merrillii’ and its response to gibberellin[J]. Journal of Zhejiang A&F University, 2025, 42(1): 103−111. DOI: 10.11833/j.issn.2095-0756.20240291.

[28]

翟俏丽, 范桂枝, 詹亚光. 真菌诱导子促进白桦悬浮细胞三萜的积累[J]. 林业科学, 2011, 47(6): 42−47.

ZHAI Qiaoli, FAN Guizhi, ZHAN Yaguang. Fungal elicitors promote triterpenoids accumulation in suspension cells of Betula platyphylla[J]. Scientia Silvae Sinicae, 2011, 47(6): 42−47. DOI: 10.11707/j.1001-7488.20110607.

[29]

BOLLER T. Chemoperception of microbial signals in plant cells[J]. Annual Review of Plant Physiology and Plant Molecular Biology, 1995, 46: 189−214. DOI: 10.1146/annurev.pp.46.060195.001201.

[30]

张彦妮, 陈素波. 长叶点地梅的细胞悬浮培养条件[J]. 草业科学, 2014, 31(8): 1481−1486.

ZHANG Yanni, CHEN Subo. Cell suspension culture conditions of Dianthus longifolia[J]. Pratacultural Science, 2014, 31(8): 1481−1486. DOI: 10.11829/j.issn.1001-0629.2013-0359.

[31]

YOUSEFI F, JABBARZADEH Z, AMIRI J, et al. Response of roses (Rosa hybrida L. ‘Herbert stevens’) to foliar application of polyamines on root development, flowering, photosynthetic pigments, antioxidant enzymes activity and NPK[J]. Scientific Reports, 2019, 9(1): 16025. DOI: 10.1038/s41598-019-52547-1.

[32]

YOUSEFI F, JABBARZADEH Z, AMIRI J, et al. Foliar application of polyamines improve some morphological and physiological characteristics of rose[J]. Folia Horticulturae, 2021, 33(1): 147−156. DOI: 10.2478/fhort-2021-0012.

[33]

TAVALLALI V, ALHAVI N, GHOLAMI H, et al. Developmental and phytochemical changes in pot marigold (Calendula officinalis L. ) using exogenous application of polyamines[J]. Plant Physiology and Biochemistry, 2022, 183: 128−137. DOI: 10.1016/j.plaphy.2022.05.011.

[34]

BADIHI L, GERAMI M, AKBARINODEH D, et al. Physio-chemical responses of exogenous calcium nanoparticle and putrescine polyamine in Saffron (Crocus sativus L. )[J]. Physiology and Molecular Biology of Plants, 2021, 27(1): 119−133. DOI: 10.1007/s12298-020-00923-x.

[35]

NAVAKOUDIS E, KOTZABASIS K. Polyamines: Α bioenergetic smart switch for plant protection and development[J]. Journal of Plant Physiology, 2022, 270: 153618. DOI: 10.1016/j.jplph.2022.153618.

[36]

王紫晴, 李飞洁, 郭玉婷, 等. 硫化氢对白桦悬浮细胞中多胺和次生代谢物积累的影响[J]. 南京林业大学学报(自然科学版), 2017, 41(6): 26−32.

WANG Ziqing, LI Feijie, GUO Yuting, et al. Effects of hydrogen sulfide on the accumulation of polyamines and secondary metabolites in suspension cells of Betula platyphylla[J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2017, 41(6): 26−32. DOI: 10.3969/j.issn.1000-2006.201610011.

[37]

DU Xiaoji, WANG Xiaogang, CHEN Jianye, et al. Putrescine maintains postharvest pitaya quality by regulating polyamine and reactive oxygen homeostasis and energy metabolism[J]. LWT, 2025, 220: 117573. DOI: 10.1016/j.lwt.2025.117573.

[38]

TIBURCIO A F, ALTABELLA T, BITRIÁN M, et al. The roles of polyamines during the lifespan of plants: from development to stress[J]. Planta, 2014, 240(1): 1−18. DOI: 10.1007/s00425-014-2055-9.

[39]

BASSARD J E, ULLMANN P, BERNIER F, et al. Phenolamides: bridging polyamines to the phenolic metabolism[J]. Phytochemistry, 2010, 71(16): 1808−1824. DOI: 10.1016/j.phytochem.2010.08.003.

[40]

GE Xiaomin, ZHAO Aiguo, LI Shasha, et al. ACC treatment induced alterations in flavonoid accumulation in Toxicodendron vernicifluum[J]. Plant Physiology and Biochemistry, 2025, 220: 109491. DOI: 10.1016/j.plaphy.2025.109491.

[41]

PÁL M, SZALAI G, GONDOR O K, et al. Unfinished story of polyamines: role of conjugation, transport and light-related regulation in the polyamine metabolism in plants[J]. Plant Science, 2021, 308: 110923. DOI: 10.1016/j.plantsci.2021.110923.

[42]

AUGUSTYNIAK B, WOJTASIK W, SAWUŁA A, et al. The effect of spermidine supplementation on polyamine metabolism and Fusarium infection in Linum usitatissimum[J]. Physiological and Molecular Plant Pathology, 2024, 134: 102453. DOI: 10.1016/j.pmpp.2024.102453.