预培养对尾巨桉DH32-29遗传转化效率的影响

王学君; 张双; 于茹恩; 杨珺; 曾炳山; 范春节; 王学君; 张双; 于茹恩; 杨珺; 曾炳山; 范春节

doi:10.11833/j.issn.2095-0756.20250347

[1]

王豁然. 桉树生物学概论[M]. 北京: 科学出版社, 2010.

WANG Huoran. A Chinese Appreciation of Eucalypts[M]. Beijing: Science Press, 2010.

[2]

黄六贴. 桉树主要病虫害及防治措施[J]. 现代农业科技, 2022(6): 80−81.

HUANG Liutie. Main diseases and insect pests of Eucalyptus and their control measures[J]. Modern Agricultural Science and Technology, 2022(6): 80−81. DOI: 10.3969/j.issn.1007-5739.2022.06.025.

[3]

DAI Ying, HU Guojian, DUPAS A, et al. Implementing the CRISPR/Cas9 technology in Eucalyptus hairy roots using wood-related genes[J]. International Journal of Molecular Sciences, 2020, 21(10): 3408. DOI: 10.3390/ijms21103408.

[4]

FEUILLET C, LAUVERGEAT V, DESWARTE C, et al. Tissue- and cell-specific expression of a cinnamyl alcohol dehydrogenase promoter in transgenic poplar plants[J]. Plant Molecular Biology, 1995, 27(4): 651−667. DOI: 10.1007/bf00020220.

[5]

陈健波, 项东云, 张建明, 等. 广西耐寒桉树育种研究现状与对策[J]. 广西林业科学, 2003, 32(1): 7−11.

CHEN Jianbo, XIANG Dongyun, ZHANG Jianming, et al. The present status of studies on cold tolerant Eucalyptus selection in Guangxi, China[J]. Guangxi Forestry Science, 2003, 32(1): 7−11. DOI: 10.19692/j.cnki.gfs.2003.01.002.

[6]

袁滔. 广西尾巨桉DH系列无性系育苗技术[J]. 南方农业, 2023, 17(15): 266−269.

YUAN Tao. DH series clone seedling breeding technology of Eucalyptus urophglla × E. grandis in Guangxi Province[J]. South China Agriculture, 2023, 17(15): 266−269. DOI: 10.19415/j.cnki.1673-890x.2023.15.064.

[7]

ALIU E, LEE K, WANG Kan. CRISPR RNA-guided integrase enables high-efficiency targeted genome engineering in Agrobacterium tumefaciens[J]. Plant Biotechnology Journal, 2022, 20(10): 1916−1927. DOI: 10.1111/pbi.13872.

[8]

徐成成, 刘锦, 吴恩, 等. 基于组织培养的木本植物器官发生型再生研究进展[J/OL]. 浙江农林大学学报, 2025-11-26. https://link.cnki.net/urlid/33.1370.S.20251126.1125.002.

XU Chengcheng, LIU Jin, WU En, et al. Advances in organogenesis regeneration of woody plants based on tissue culture[J/OL]. Journal of Zhejiang A&F University, 2025-11-26. https://link.cnki.net/urlid/33.1370.S.20251126.1125.002.

[9]

WANG Haihai, WANG Cuiting, LIU Hua, et al. An efficient Agrobacterium-mediated transformation and regeneration system for leaf explants of two elite aspen hybrid clones Populus alba × P. berolinensis and Populus davidiana × P. bolleana[J]. Plant Cell Reports, 2011, 30(11): 2037−2044. DOI: 10.1007/s00299-011-1111-1.

[10]

唐靖雯, 王宁, 伍程程, 等. 白花玉石籽石榴遗传转化体系的建立[J]. 果树学报, 2024, 41(12): 2621−2633.

TANG Jingwen, WANG Ning, WU Chengcheng, et al. Establishment of genetic transformation system for Baihuayushizi pomegranate[J]. Journal of Fruit Science, 2024, 41(12): 2621−2633. DOI: 10.13925/j.cnki.gsxb.20240326.

[11]

JACQ B, LESOBRE O, SANGWAN R, et al. Factors influencing T-DNA transfer in Agrobacterium-mediated transformation of sugarbeet[J]. Plant Cell Reports, 1993, 12(11): 621−624. DOI: 10.1007/bf00232811.

[12]

WEN Shuangshuang, GE Xiaolan, WANG Rui, et al. An efficient Agrobacterium-mediated transformation method for hybrid poplar 84K (Populus alba × P. glandulosa) using calli as explants[J]. International Journal of Molecular Sciences, 2022, 23(4): 2216. DOI: 10.3390/ijms23042216.

[13]

甘珊珊, 王静毅, 程运江, 等. 香蕉遗传转化体系优化[J]. 热带农业科学, 2024, 44(10): 17−24.

GAN Shanshan, WANG Jingyi, CHENG Yunjiang, et al. Optimization of the banana genetic transformation system[J]. Chinese Journal of Tropical Agriculture, 2024, 44(10): 17−24.

[14]

HAN Xue, MA Shurong, KONG Xianghui, et al. Efficient Agrobacterium-mediated transformation of hybrid poplar Populus davidiana Dode × Populus bollena Lauche[J]. International Journal of Molecular Sciences, 2013, 14(2): 2515−2528. DOI: 10.3390/ijms14022515.

[15]

XIA Yufei, CAO Yuan, REN Yongyu, et al. Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method[J]. Plant Methods, 2023, 19: 15. DOI: 10.1186/s13007-023-00994-3.

[16]

LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^–ΔΔCt method[J]. Methods, 2001, 25(4): 402−408. DOI: 10.1006/meth.2001.1262.

[17]

DEININGER P. Gus protocols: using the gus gene as a reporter of gene expression[J]. Analytical Biochemistry, 1992, 207(2): 356. DOI: 10.1016/0003-2697(92)90027-5.

[18]

薛薇. 基于SPSS的数据分析[M]. 北京: 中国人民大学出版社, 2006.

XUE Wei. Data Analysis based on SPSS[M]. Beijing: China Renmin University Press, 2006.

[19]

CAVANAGH B L, WALKER T, NORAZIT A, et al. Thymidine analogues for tracking DNA synthesis[J]. Molecules, 2011, 16(9): 7980−7993. DOI: 10.3390/molecules16097980.

[20]

WANG Xiaoping, CHEN Shanshan, ZHANG Haonan, et al. Agrobacterium-mediated genetic transformation of the most widely cultivated superior clone Eucalyptus urophylla × Eucalyptus grandis DH32-29 in Southern China[J]. Frontiers in Plant Science, 2022, 13: 1011245. DOI: 10.3389/fpls.2022.1011245.

[21]

陈珊珊. 尾巨桉DH32-29不定芽再生机制及遗传转化体系优化的研究[D]. 哈尔滨: 东北林业大学, 2023.

CHEN Shanshan. Study on the Mechanism of in vitro Shoot Regeneration and Optimization of Genetic Transformation System in Eucalyptus grandis × E. urophylla[D]. Haerbin: Northeast Forestry University, 2023. DOI: 10.27009/d.cnki.gdblu.2023.001039.

[22]

陈梅, 陈露倩, 陈思, 等. 农杆菌介导的黄瓜遗传转化体系优化研究[J]. 四川农业大学学报, 2024, 42(3): 540−545, 571.

CHEN Mei, CHEN Luqian, CHEN Si, et al. Optimization of the genetic transformation system of cucumber mediated by Agrobacterium tumefaciens[J]. Journal of Sichuan Agricultural University, 2024, 42(3): 540−545, 571. DOI: 10.16036/j.issn.1000-2650.202310365.

[23]

刘闵豪, 徐郡儡, 叶靖, 等. 农杆菌介导的杜仲叶片愈伤组织遗传转化体系[J]. 林业科学, 2020, 56(2): 79−88.

LIU Minhao, XU Junlei, YE Jing, et al. Agrobacterium tumefaciens-mediated transformation of leaf callus in Eucommia ulmoides[J]. Scientia Silvae Sinicae, 2020, 56(2): 79−88. DOI: 10.11707/j.1001-7488.20200209.

[24]

LONG Fenfang, ZUO Weiwei, LI Huie, et al. Genetic transformation of Rhododendron delavayi for anthocyanin synthesis using Agrobacterium-mediated transformation[J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2024, 157(2): 47. DOI: 10.1007/s11240-024-02775-9.

[25]

邱实, 张文举, 许馥慧, 等. Micro-Tom番茄转基因植株再生体系的优化[J]. 上海大学学报(自然科学版), 2018, 24(2): 322−330.

QIU Shi, ZHANG Wenju, XU Fuhui, et al. System optimization for tomato Micro-Tom transgenic regeneration[J]. Journal of Shanghai University (Natural Science Edition), 2018, 24(2): 322−330. DOI: 10.12066/j.issn.1007-2861.1773.

[26]

张杨, 诸燕, 韩之刚, 等. 过表达紫花苜蓿ChOMT基因创制高异黄酮营养强化番茄[J]. 浙江农林大学学报, 2026, 43(2): 303−309.

ZHANG Yang, ZHU Yan, HAN Zhigang, et al. Overexpression of Medicago sativa ChOMT gene in constructing homoisoflavone-enriched tomatoes[J]. Journal of Zhejiang A&F University, 2026, 43(2): 303−309. DOI: 10.11833/j.issn.2095-0756.20250186.

[27]

HUSAINI A M. Pre- and post-agroinfection strategies for efficient leaf disk transformation and regeneration of transgenic strawberry plants[J]. Plant Cell Reports, 2010, 29(1): 97−110. DOI: 10.1007/s00299-009-0801-4.

[28]

ISMAGUL A, MAZONKA I, CALLEGARI C, et al. Agrobacterium-mediated transformation of barley (Hordeum vulgare L.)[M]//FLEURY D, WHITFORD R. Crop Breeding. Methods in Molecular Biology , vol 1145. New York: Humana Press, 2014: 203−211. DOI:10.1007/978-1-4939-0446-4_16.

[1]	王豁然. 桉树生物学概论[M]. 北京: 科学出版社, 2010. WANG Huoran. A Chinese Appreciation of Eucalypts[M]. Beijing: Science Press, 2010.
[2]	黄六贴. 桉树主要病虫害及防治措施[J]. 现代农业科技, 2022(6): 80−81. HUANG Liutie. Main diseases and insect pests of Eucalyptus and their control measures[J]. Modern Agricultural Science and Technology, 2022(6): 80−81. DOI: 10.3969/j.issn.1007-5739.2022.06.025.
[3]	DAI Ying, HU Guojian, DUPAS A, et al. Implementing the CRISPR/Cas9 technology in Eucalyptus hairy roots using wood-related genes[J]. International Journal of Molecular Sciences, 2020, 21(10): 3408. DOI: 10.3390/ijms21103408.
[4]	FEUILLET C, LAUVERGEAT V, DESWARTE C, et al. Tissue- and cell-specific expression of a cinnamyl alcohol dehydrogenase promoter in transgenic poplar plants[J]. Plant Molecular Biology, 1995, 27(4): 651−667. DOI: 10.1007/bf00020220.
[5]	陈健波, 项东云, 张建明, 等. 广西耐寒桉树育种研究现状与对策[J]. 广西林业科学, 2003, 32(1): 7−11. CHEN Jianbo, XIANG Dongyun, ZHANG Jianming, et al. The present status of studies on cold tolerant Eucalyptus selection in Guangxi, China[J]. Guangxi Forestry Science, 2003, 32(1): 7−11. DOI: 10.19692/j.cnki.gfs.2003.01.002.
[6]	袁滔. 广西尾巨桉DH系列无性系育苗技术[J]. 南方农业, 2023, 17(15): 266−269. YUAN Tao. DH series clone seedling breeding technology of Eucalyptus urophglla × E. grandis in Guangxi Province[J]. South China Agriculture, 2023, 17(15): 266−269. DOI: 10.19415/j.cnki.1673-890x.2023.15.064.
[7]	ALIU E, LEE K, WANG Kan. CRISPR RNA-guided integrase enables high-efficiency targeted genome engineering in Agrobacterium tumefaciens[J]. Plant Biotechnology Journal, 2022, 20(10): 1916−1927. DOI: 10.1111/pbi.13872.
[8]	徐成成, 刘锦, 吴恩, 等. 基于组织培养的木本植物器官发生型再生研究进展[J/OL]. 浙江农林大学学报, 2025-11-26. https://link.cnki.net/urlid/33.1370.S.20251126.1125.002. XU Chengcheng, LIU Jin, WU En, et al. Advances in organogenesis regeneration of woody plants based on tissue culture[J/OL]. Journal of Zhejiang A&F University, 2025-11-26. https://link.cnki.net/urlid/33.1370.S.20251126.1125.002.
[9]	WANG Haihai, WANG Cuiting, LIU Hua, et al. An efficient Agrobacterium-mediated transformation and regeneration system for leaf explants of two elite aspen hybrid clones Populus alba × P. berolinensis and Populus davidiana × P. bolleana[J]. Plant Cell Reports, 2011, 30(11): 2037−2044. DOI: 10.1007/s00299-011-1111-1.
[10]	唐靖雯, 王宁, 伍程程, 等. 白花玉石籽石榴遗传转化体系的建立[J]. 果树学报, 2024, 41(12): 2621−2633. TANG Jingwen, WANG Ning, WU Chengcheng, et al. Establishment of genetic transformation system for Baihuayushizi pomegranate[J]. Journal of Fruit Science, 2024, 41(12): 2621−2633. DOI: 10.13925/j.cnki.gsxb.20240326.
[11]	JACQ B, LESOBRE O, SANGWAN R, et al. Factors influencing T-DNA transfer in Agrobacterium-mediated transformation of sugarbeet[J]. Plant Cell Reports, 1993, 12(11): 621−624. DOI: 10.1007/bf00232811.
[12]	WEN Shuangshuang, GE Xiaolan, WANG Rui, et al. An efficient Agrobacterium-mediated transformation method for hybrid poplar 84K (Populus alba × P. glandulosa) using calli as explants[J]. International Journal of Molecular Sciences, 2022, 23(4): 2216. DOI: 10.3390/ijms23042216.
[13]	甘珊珊, 王静毅, 程运江, 等. 香蕉遗传转化体系优化[J]. 热带农业科学, 2024, 44(10): 17−24. GAN Shanshan, WANG Jingyi, CHENG Yunjiang, et al. Optimization of the banana genetic transformation system[J]. Chinese Journal of Tropical Agriculture, 2024, 44(10): 17−24.
[14]	HAN Xue, MA Shurong, KONG Xianghui, et al. Efficient Agrobacterium-mediated transformation of hybrid poplar Populus davidiana Dode × Populus bollena Lauche[J]. International Journal of Molecular Sciences, 2013, 14(2): 2515−2528. DOI: 10.3390/ijms14022515.
[15]	XIA Yufei, CAO Yuan, REN Yongyu, et al. Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method[J]. Plant Methods, 2023, 19: 15. DOI: 10.1186/s13007-023-00994-3.
[16]	LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^–ΔΔCt method[J]. Methods, 2001, 25(4): 402−408. DOI: 10.1006/meth.2001.1262.
[17]	DEININGER P. Gus protocols: using the gus gene as a reporter of gene expression[J]. Analytical Biochemistry, 1992, 207(2): 356. DOI: 10.1016/0003-2697(92)90027-5.
[18]	薛薇. 基于SPSS的数据分析[M]. 北京: 中国人民大学出版社, 2006. XUE Wei. Data Analysis based on SPSS[M]. Beijing: China Renmin University Press, 2006.
[19]	CAVANAGH B L, WALKER T, NORAZIT A, et al. Thymidine analogues for tracking DNA synthesis[J]. Molecules, 2011, 16(9): 7980−7993. DOI: 10.3390/molecules16097980.
[20]	WANG Xiaoping, CHEN Shanshan, ZHANG Haonan, et al. Agrobacterium-mediated genetic transformation of the most widely cultivated superior clone Eucalyptus urophylla × Eucalyptus grandis DH32-29 in Southern China[J]. Frontiers in Plant Science, 2022, 13: 1011245. DOI: 10.3389/fpls.2022.1011245.
[21]	陈珊珊. 尾巨桉DH32-29不定芽再生机制及遗传转化体系优化的研究[D]. 哈尔滨: 东北林业大学, 2023. CHEN Shanshan. Study on the Mechanism of in vitro Shoot Regeneration and Optimization of Genetic Transformation System in Eucalyptus grandis × E. urophylla[D]. Haerbin: Northeast Forestry University, 2023. DOI: 10.27009/d.cnki.gdblu.2023.001039.
[22]	陈梅, 陈露倩, 陈思, 等. 农杆菌介导的黄瓜遗传转化体系优化研究[J]. 四川农业大学学报, 2024, 42(3): 540−545, 571. CHEN Mei, CHEN Luqian, CHEN Si, et al. Optimization of the genetic transformation system of cucumber mediated by Agrobacterium tumefaciens[J]. Journal of Sichuan Agricultural University, 2024, 42(3): 540−545, 571. DOI: 10.16036/j.issn.1000-2650.202310365.
[23]	刘闵豪, 徐郡儡, 叶靖, 等. 农杆菌介导的杜仲叶片愈伤组织遗传转化体系[J]. 林业科学, 2020, 56(2): 79−88. LIU Minhao, XU Junlei, YE Jing, et al. Agrobacterium tumefaciens-mediated transformation of leaf callus in Eucommia ulmoides[J]. Scientia Silvae Sinicae, 2020, 56(2): 79−88. DOI: 10.11707/j.1001-7488.20200209.
[24]	LONG Fenfang, ZUO Weiwei, LI Huie, et al. Genetic transformation of Rhododendron delavayi for anthocyanin synthesis using Agrobacterium-mediated transformation[J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2024, 157(2): 47. DOI: 10.1007/s11240-024-02775-9.
[25]	邱实, 张文举, 许馥慧, 等. Micro-Tom番茄转基因植株再生体系的优化[J]. 上海大学学报(自然科学版), 2018, 24(2): 322−330. QIU Shi, ZHANG Wenju, XU Fuhui, et al. System optimization for tomato Micro-Tom transgenic regeneration[J]. Journal of Shanghai University (Natural Science Edition), 2018, 24(2): 322−330. DOI: 10.12066/j.issn.1007-2861.1773.
[26]	张杨, 诸燕, 韩之刚, 等. 过表达紫花苜蓿ChOMT基因创制高异黄酮营养强化番茄[J]. 浙江农林大学学报, 2026, 43(2): 303−309. ZHANG Yang, ZHU Yan, HAN Zhigang, et al. Overexpression of Medicago sativa ChOMT gene in constructing homoisoflavone-enriched tomatoes[J]. Journal of Zhejiang A&F University, 2026, 43(2): 303−309. DOI: 10.11833/j.issn.2095-0756.20250186.
[27]	HUSAINI A M. Pre- and post-agroinfection strategies for efficient leaf disk transformation and regeneration of transgenic strawberry plants[J]. Plant Cell Reports, 2010, 29(1): 97−110. DOI: 10.1007/s00299-009-0801-4.
[28]	ISMAGUL A, MAZONKA I, CALLEGARI C, et al. Agrobacterium-mediated transformation of barley (Hordeum vulgare L.)[M]//FLEURY D, WHITFORD R. Crop Breeding. Methods in Molecular Biology , vol 1145. New York: Humana Press, 2014: 203−211. DOI:10.1007/978-1-4939-0446-4_16.