山核桃种子脂肪代谢期EST序列的初步分析

黄银芝; 曾燕如; 周秦; 夏国华; 黄有军

doi:10.11833/j.issn.2095-0756.2015.02.009

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山核桃种子脂肪代谢期EST序列的初步分析

黄银芝 , 曾燕如 , 周秦 , 夏国华 , 黄有军

文章导航 > 浙江农林大学学报 > 2015 > 32(2): 229-236

黄银芝, 曾燕如, 周秦, 夏国华, 黄有军. 山核桃种子脂肪代谢期EST序列的初步分析[J]. 浙江农林大学学报, 2015, 32(2): 229-236. doi: 10.11833/j.issn.2095-0756.2015.02.009

引用本文:

HUANG Yinzhi, ZENG Yanru, ZHOU Qin, XIA Guohua, HUANG Youjun. Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis[J]. Journal of Zhejiang A&F University, 2015, 32(2): 229-236. doi: 10.11833/j.issn.2095-0756.2015.02.009

Citation:

HUANG Yinzhi, ZENG Yanru, ZHOU Qin, XIA Guohua, HUANG Youjun. Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis[J]. Journal of Zhejiang A&F University, 2015, 32(2): 229-236. doi: 10.11833/j.issn.2095-0756.2015.02.009

山核桃种子脂肪代谢期EST序列的初步分析

doi: 10.11833/j.issn.2095-0756.2015.02.009

1.
浙江农林大学亚热带森林培育国家重点实验室培育基地，浙江临安 311300;
2.
浙江省黄岩区头陀镇人民政府农业办公室，浙江黄岩 318026;
3.
浙江省金华市农业科学研究院，浙江金华 321017

基金项目:

浙江省自然科学基金重点项目（Z13C160012）;浙江省科学技术创新团队项目（2011R50030）;国家高技术研究发展计划（863计划）项目（2013AA102605）

详细信息

作者简介: 黄银芝，从事经济林培育与利用研究。

通信作者: 曾燕如，教授，博士，从事经济林培育与利用研究。

中图分类号: S722.3；Q753

Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis

1.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;
2.
Agricultural Office of Toutuo People’s Government, Huangyan District, Huangyan 318026, Zhejiang, China;
3.
Jinhua Institute of Agricultural Sciences, Jinhua 321017, Zhejiang, China

摘要: 山核桃Carya cathayensis为重要的油料干果树种，其非同一般的高含油率必定有其特殊的成油机制。为探索其成油机制，在已构建的山核桃脂肪代谢相关cDNA文库基础上，对cDNA进行随机克隆测序，对序列进行拼接、功能注释，基因本体论（gene ontology，GO）分类及基于拟南芥Arabidopsis thaliana的FunCat分类，并对与脂肪代谢相关的全长cDNA序列进行蛋白性质分析。结果共得到1 010条山核桃表达序列标签（ESTs），经拼接获得188个编码蛋白质的基因（unigene），其中92个contigs和96个singlets。GO分类将这些unigene分成细胞组分、分子功能和生物过程等3类，并发现各序列编码的基因并不承担单一的功能，有些序列在功能上有重叠；同时参照拟南芥的基因功能分类，将unigene序列在功能上分成9类，得到了143个全长cDNA序列，其中包括14个与脂肪代谢相关的cDNA序列。对12个与脂肪代谢相关的全长cDNA序列进行蛋白性质分析，发现它们均具有磷酸化位点；除油质蛋白外都没有跨膜区域，且不具信号肽结构，为非分泌蛋白，属于某个蛋白家族；不同的油体蛋白具有不同的跨膜区，且均具有信号肽结构，属于Oleosin super family蛋白家族。表4参35
- 经济林学 /
- 山核桃 /
- 表达序列标签 /
- 功能分类 /
- 脂肪代谢 /
- 蛋白分析
Abstract: Carya cathayensis is an important oil species with dry nuts that are high oil content, for which there must be some special oil-synthesizing mechanism. In order to explore the mechanism, random cloning and sequencing of cDNAs from a fatty acid metabolism-associated cDNA library, sequence assembly and annotation, Gene Ontology （GO）- and Arabidopsis thaliana-associated MIPS Functional Catalogue （FunCat）-based classification of assembled sequences, and protein analysis of fatty acid metabolism-associated cDNA sequences were performed. A total of 1 010 expressed sequence tags （ESTs） was obtained, based on which 188 unigenes were assembled. There were 92 contigs and 96 singlets among the unigenes, which were classified into categories of cellular component, molecular function, and biological process based on classification of GO and found to overlap one another in functions. But against MIPS Functional Catalogue in Arabidopsis thaliana, these unigenes were classified into nine categories and 143 full-length cDNAs were obtained, from which 14 cDNA sequences were associated with metabolism of fatty acids. It had been found through proteomic analysis that 12 full-length fatty acid metabolism-related cDNAs had a phosphorylation site; they were all non-secretory proteins with no transmembrane domain and signal peptide except oleosins, belonging to certain protein family; and oleosins had different transmembrane domains and a signal peptide, belonging to a super oleosin family. ［Ch, 4 tab. 35 ref.］
- cash forestry /
- Carya cathayensis /
- expressed sequence tag /
- functional classification /
- fatty acid metabolism /
- protein analysis

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山核桃种子脂肪代谢期EST序列的初步分析

doi: 10.11833/j.issn.2095-0756.2015.02.009

1. 浙江农林大学亚热带森林培育国家重点实验室培育基地，浙江临安 311300;

2. 浙江省黄岩区头陀镇人民政府农业办公室，浙江黄岩 318026;

3. 浙江省金华市农业科学研究院，浙江金华 321017

基金项目:

浙江省自然科学基金重点项目（Z13C160012）;浙江省科学技术创新团队项目（2011R50030）;国家高技术研究发展计划（863计划）项目（2013AA102605）

作者简介:
黄银芝，从事经济林培育与利用研究。

通信作者: 曾燕如，教授，博士，从事经济林培育与利用研究。

收稿日期: 2014-06-12
修回日期: 2014-08-24
刊出日期: 2015-04-20

中图分类号: S722.3；Q753

关键词:

摘要: 山核桃Carya cathayensis为重要的油料干果树种，其非同一般的高含油率必定有其特殊的成油机制。为探索其成油机制，在已构建的山核桃脂肪代谢相关cDNA文库基础上，对cDNA进行随机克隆测序，对序列进行拼接、功能注释，基因本体论（gene ontology，GO）分类及基于拟南芥Arabidopsis thaliana的FunCat分类，并对与脂肪代谢相关的全长cDNA序列进行蛋白性质分析。结果共得到1 010条山核桃表达序列标签（ESTs），经拼接获得188个编码蛋白质的基因（unigene），其中92个contigs和96个singlets。GO分类将这些unigene分成细胞组分、分子功能和生物过程等3类，并发现各序列编码的基因并不承担单一的功能，有些序列在功能上有重叠；同时参照拟南芥的基因功能分类，将unigene序列在功能上分成9类，得到了143个全长cDNA序列，其中包括14个与脂肪代谢相关的cDNA序列。对12个与脂肪代谢相关的全长cDNA序列进行蛋白性质分析，发现它们均具有磷酸化位点；除油质蛋白外都没有跨膜区域，且不具信号肽结构，为非分泌蛋白，属于某个蛋白家族；不同的油体蛋白具有不同的跨膜区，且均具有信号肽结构，属于Oleosin super family蛋白家族。表4参35

English Abstract

Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;

2. Agricultural Office of Toutuo People’s Government, Huangyan District, Huangyan 318026, Zhejiang, China;

3. Jinhua Institute of Agricultural Sciences, Jinhua 321017, Zhejiang, China

Received Date: 2014-06-12
Rev Recd Date: 2014-08-24
Publish Date: 2015-04-20

Keywords:

Abstract: Carya cathayensis is an important oil species with dry nuts that are high oil content, for which there must be some special oil-synthesizing mechanism. In order to explore the mechanism, random cloning and sequencing of cDNAs from a fatty acid metabolism-associated cDNA library, sequence assembly and annotation, Gene Ontology （GO）- and Arabidopsis thaliana-associated MIPS Functional Catalogue （FunCat）-based classification of assembled sequences, and protein analysis of fatty acid metabolism-associated cDNA sequences were performed. A total of 1 010 expressed sequence tags （ESTs） was obtained, based on which 188 unigenes were assembled. There were 92 contigs and 96 singlets among the unigenes, which were classified into categories of cellular component, molecular function, and biological process based on classification of GO and found to overlap one another in functions. But against MIPS Functional Catalogue in Arabidopsis thaliana, these unigenes were classified into nine categories and 143 full-length cDNAs were obtained, from which 14 cDNA sequences were associated with metabolism of fatty acids. It had been found through proteomic analysis that 12 full-length fatty acid metabolism-related cDNAs had a phosphorylation site; they were all non-secretory proteins with no transmembrane domain and signal peptide except oleosins, belonging to certain protein family; and oleosins had different transmembrane domains and a signal peptide, belonging to a super oleosin family. ［Ch, 4 tab. 35 ref.］

引用本文: