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摘要: 为研究兰花MADS-box基因的表达与花器官发育之间的关系，以文心兰百万金币Oncidium Milliongolds为材料，通过RT-PCR （reverse transcription polymerase chain reaction） 方法克隆到1个花发育相关的MADS-box基因，命名为OAP 3（GenBank登录号为GU644447）。该基因全长799 bp，编码204个氨基酸，具有典型的MADS结构域和K结构域，与拟南芥Arabidopsis thaliana等物种的AP 3-like同源基因所编码的氨基酸同源性达到50%～78%。进化树分析表明：OAP 3属于花器官发育B类基因，但该基因却不存在B类基因C-端共有的结构域。逆转录聚合酶链式扩增反应（qRT-PCR，quantitative RT-PCR）表达分析表明：从抽花开始，随花芽的生长，该基因相对表达丰度呈明显上升趋势；OAP 3在文心兰萼片、花瓣、唇瓣、雄蕊、雌蕊以及营养器官叶片和花茎中都表达。图5表1参17Abstract: To investigate the expression pattern of MADS-box gene during the development of the orchids flower，the floral organ identity gene OAP 3 with a MADS-box motif was cloned from an orchid plant through the RT-PCR，OncidiumMilliongolds（GenBank accession No.GU644447）. Then a sequence analysis and a phylogenetic analysis were conducted along with a quantitative real-time polymerase chain reaction （qRT-PCR）. The full length cDNA of OAP 3 was 799 bp with an open reading frame（ORF） encoding 204 amino acids. The deduced amino acids sequence contained a typical MADS domain and a K domain，and the sequence analysis indicated that OAP 3 shared 50%-78% homology in the amino acid sequence with Arabidopsis thaliana and other species of APETALA3 homologues. The phylogenetic analysis suggested that OAP 3 belonged to the B gene，but four consensus motifs identified in the C-terminal region of the B group genes were absent in the OAP 3 gene. The qRT-PCR analysis showed that OAP 3 mRNA increased with flower bud development，as well as in all four floral organs，vegetative leaves，and shoots.［Ch，5 fig. 1 tab. 17 ref.］
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Key words:
- molecular biology /
- Oncidium‘Milliongolds’ /
- floral initiation /
- MADS-box genes /
- OAP 3 gene /
- qRT-PCR
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2011.06.010
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