Cloning and expression of polyphenol oxidase （<em>PPO</em>） in <em>Phyllostachys nigra</em>

WANG Yi; GUO Xiao-qin; LOU Yong-feng; YANG Hai-yun; LIN Xin-chun; FANG Wei

doi:10.11833/j.issn.2095-0756.2010.05.023

Volume 27 Issue 5

Oct. 2010

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Article Navigation > Journal of Zhejiang A&F University > 2010 > 27(5): 781-785

WANG Yi, GUO Xiao-qin, LOU Yong-feng, et al. Cloning and expression of polyphenol oxidase （PPO） in Phyllostachys nigra[J]. Journal of Zhejiang A&F University, 2010, 27(5): 781-785. DOI: 10.11833/j.issn.2095-0756.2010.05.023

Citation:

WANG Yi, GUO Xiao-qin, LOU Yong-feng, et al. Cloning and expression of polyphenol oxidase （PPO） in Phyllostachys nigra[J]. Journal of Zhejiang A&F University, 2010, 27(5): 781-785. DOI: 10.11833/j.issn.2095-0756.2010.05.023

Cloning and expression of polyphenol oxidase （PPO） in Phyllostachys nigra

DOI: 10.11833/j.issn.2095-0756.2010.05.023

1.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University， Lin’an 311300，Zhejiang，China

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Corresponding author: GUO Xiao-qin
Publish Date: 2010-10-20

Abstract

To study the relationship between polyphenol oxidase （PPO） and tissue browning in Phyllostachys nigra，homologous cloning was used to obtain a partial sequence of the PPO gene in Phyllostachys nigra （named PnPPO）. The gene structure and the expression pattern in tissue cultured seedlings with different degrees of browning were checked using reverse transcription Polymerase Chain Reaction（RT-PCR）. The sequence revealed a homolog of the PPO gene in Oryza sativa and Triticum aestivum with the amino acid belonging to tyrosinase. Also，RT-PCR results showed that with high levels of browning，PnPPO had strong expression. Thus，the expression of PnPPO was related to the degree of browning.［Ch，4 fig. 15 ref.］
- forest biology,
- polyphenol oxidase,
- Phyllostachys nigra,
- tissue browning

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Cloning and expression of polyphenol oxidase （PPO） in Phyllostachys nigra

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University， Lin’an 311300，Zhejiang，China

Corresponding author: GUO Xiao-qin

Publish Date: 2010-10-20

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Abstract: To study the relationship between polyphenol oxidase （PPO） and tissue browning in Phyllostachys nigra，homologous cloning was used to obtain a partial sequence of the PPO gene in Phyllostachys nigra （named PnPPO）. The gene structure and the expression pattern in tissue cultured seedlings with different degrees of browning were checked using reverse transcription Polymerase Chain Reaction（RT-PCR）. The sequence revealed a homolog of the PPO gene in Oryza sativa and Triticum aestivum with the amino acid belonging to tyrosinase. Also，RT-PCR results showed that with high levels of browning，PnPPO had strong expression. Thus，the expression of PnPPO was related to the degree of browning.［Ch，4 fig. 15 ref.］

Cloning and expression of polyphenol oxidase （PPO） in Phyllostachys nigra

DOI: 10.11833/j.issn.2095-0756.2010.05.023

Abstract

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通讯作者: 陈斌, bchen63@163.com

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Cloning and expression of polyphenol oxidase （PPO） in Phyllostachys nigra

doi: 10.11833/j.issn.2095-0756.2010.05.023

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University， Lin’an 311300，Zhejiang，China

Corresponding author: GUO Xiao-qin

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Cloning and expression of polyphenol oxidase （PPO） in Phyllostachys nigra

DOI: 10.11833/j.issn.2095-0756.2010.05.023

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通讯作者: 陈斌, bchen63@163.com

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Cloning and expression of polyphenol oxidase （PPO） in Phyllostachys nigra

doi: 10.11833/j.issn.2095-0756.2010.05.023

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University， Lin’an 311300，Zhejiang，China

Corresponding author: GUO Xiao-qin

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