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Articles in press have been peer-reviewed and accepted, which are not yet assigned to volumes/issues, but are citable by Digital Object Identifier (DOI).
Effects of the tourists’ place perception and environmental behavior on health benefit evaluation at Fuzhou National Forest Park
ZHU Jinli, LIN Xiaoqiu, SHAO Hongrui, LI Wenxiao, DONG Jianwen, ZHENG Yu
, Available online  doi: 10.11833/j.issn.2095-0756.20230534
Abstract:
  Objective  Place attachment and environmental behavior are the results of the interaction between tourists and the environment, closely related to the construction of landscape perception, and of great significance for exploring the impact mechanism of the forest parks and tourists’ health. This paper explored the internal transformation mechanism of the forest parks’ health benefits from the perspectives of tourists’ place perception and environmental behavior.   Method  Taking Fuzhou National Forest Park as an example, this paper combined the perspective of tourists’ perceived evaluation, emotion response, and behavior practice, to construct the relational model of landscape perception, place attachment, environmental behavior, and health benefit assessment. Finally, analysed the influence relationship between the four through the structural equation model.   Result  (1) The perception of natural attributes, facility attributes, cultural attributes, and spatial attributes are the four dimensions that constitute the landscape perception. The influence coefficients are 0.777, 0.696, 0.743, and 0.755. Landscape perception has significant correlation with place attachment, with an impact degree of 74.4%. (2) Landscape perception has a significant impact on environmental behavior, with a direct impact effect of 39.8%, and an indirect impact effect of 44.3%. Place attachment is a mediator variable in the relationship between the two. (3) Landscape perception has a significant impact on health benefit evaluation, with a direct impact effect of 30.2%, and an indirect impact of 50.9% is achieved through the mediator effect of place attachment and environmental behavior.   Conclusion  Through the construction and testing analysis of structural equation model testing, this paper revealed the impact mechanism of forest park and tourists’ health benefit, is a composite system formed by the interaction elements of “perceived evaluation, emotional response, behavioral practice”. It is necessary to combine media data and scientific human health testing equipment to expand the depth and breadth of research in future. [Ch, 1 fig. 6 tab. 30 ref.]
Genome-wide identification of the ICE gene family in moso bamboo and its expression pattern under low temperature stress
WANG Shuwei, ZHOU Mingbing
, Available online  doi: 10.11833/j.issn.2095-0756.20230445
Abstract:
  Objective  The ICE gene family of Phyllostachys edulis (moso bamboo) was identified and analyzed to find out the key family members responding to cold resistance of moso bamboo, and to lay a theoretical foundation for further research on the biological functions of moso bamboo ICE genes, the molecular mechanisms responding to low-temperature stress, and the genetic transformation to improve the cold resistance of moso bamboo.   Method  Bioinformatics methods were used to identify and analyze the ICE gene family members of moso bamboo, and to analyze the physiological indexes and the expression patterns of ICE genes in moso bamboo at 0, 0.5, 1.0, 24.0, and 48.0 h of low-temperature treatments at 4, 0 and −2 ℃.   Result  A total of four mosaic bamboo ICE genes were identified. Conserved structural domains and multiple sequence comparison analyses showed that their structures were highly similar. Phylogenetic relationships and promoter cis-acting element analyses showed that the PeICE gene was more closely related to rice, and a large number of cis-acting elements associated with abiotic stresses were also present. Reactive oxygen radical (superoxide anion) staining revealed that the ROS staining gradually deepened with the increase in treatment time, but its staining gradually weakened after 24.0 h of 0 ℃ treatment and 1.0 h of −2 ℃ treatment. Proline content and superoxide dismutase activity assays revealed an overall increase in Pro and SOD content at 4 ℃ and 0 ℃, but their expression was lower than the control at −2 ℃, and peroxidase activity assays revealed an increase at all three temperatures. Analysis of ICE gene expression pattern revealed that the overall expression of PeICE increased in 4 ℃ and 0 ℃ treatment, and the most obvious increase was in PeICE3. The overall expression level of PeICE was lower than that of the control at −2.0 ℃.   Conclusion  With the decrease of cold treatment temperature and the enhancement of treatment time, moso bamboo was subjected to increasing damage, and its in vivo enzyme activity system as well as ICE genes responded positively to cold stress, and PeICE3 was the most sensitive to cold stress, whereas the expression of ICE genes did not increase at −2 ℃, and it was hypothesized that this gene family responded to low-temperature stress instead of freezing stress. [Ch, 7 fig. 1 tab. 42 ref.]
Sources and contamination assessment of heavy metals in the green land soils in Tongzhou District, Beijing
SUN Jingyu, SUN Xiangyang, LI Suyan, WANG Chenchen, YUE Zongwei
, Available online  doi: 10.11833/j.issn.2095-0756.20230435
Abstract:
  Objective  The types of land use in Tongzhou District of Beijing have changed greatly. Heavy metals in the soil of green land were investigated, source analysis and risk assessment were carried out to understand whether the current use of green land meets the requirements of cleanliness and safety, and suggestions on risk prevention and control of heavy metals were given.   Method  The pH values and total amounts of heavy metals including Cd, Cr, Pb, Hg, As, Cu, and Zn in 60 surface soil samples from the study area were determined. Source apportionment was carried out using Principal Component Analysis and the Absolute Principal Component Scores-Multiple Linear Regression (APCS-MLR) receptor model. The assessment of heavy metal element pollution was conducted using the Geo-accumulation Index method and the Potential Ecological Risk Assessment method.   Result  The average contents of 7 heavy metals Cd, Cr, Pb, Hg, As, Cu and Zn in the soil were 0.53, 47.87, 11.95, 0.25, 6.71, 4.64 and 51.21 mg·kg−1, while the average content of the remaining elements were below the background. All of the samples’ heavy metal concentrations were less than the screening values for Soil Pollution Risk Control Standards for Agricultural Land (GB 15618−2018). Principal component analysis demonstrated that Cd, Pb, Hg and As were influenced by human sources, including coal combustion, traffic pollution, industrial and agricultural activities pollution; Zn originated from natural sources and was related to the soil parent material; Cu and Cr were mixed sources reaulted by soil parent materials and agricultural pollution. The contribution rates of sources calculated by APCS-MLR were as follows, a certain amount of Cd accumulated on the surface came mostly from human sources (92%); Hg was from source 3 (29%), source 1 (17%), source 4 (13%), and there were also 35% unknown sources, which were suggested as mixed sources. Moreover, sources 1, 3, and 4 were all anthropogenic sources, while source 2 was a natural source. Environmental quality evaluation of green soil was investigated through the ground accumulation index, which illustrated that Cd and Hg performed a higher ecological risk response. Most of the comprehensive potential risk values in the study area were slight and medium potential ecological risks, only few points were belonged to severe ecological risks.   Conclusion  The study shows that the overall risk level of green land soil in Tongzhou District is in the middle and low risk. The main elements causing the risk are Cd and Hg, which are the priority control elements of ecological risk, and can be controlled from the control of coal burning, traffic and industrial emissions. [Ch, 5 fig. 4 tab. 32 ref.]
Impact of site factors on forest regeneration in Mount Tianmu mixed coniferous and broad-leaved forests
YE Zilin, DOU Xiaowen, TANG Mengping
, Available online  doi: 10.11833/j.issn.2095-0756.20230446
Abstract:
  Objective  The objective is to study the impact of site factors on forest regeneration in the mixed coniferous and broad-leaved forests in Mount Tianmu, and provide a theoretical basis for the management of subtropical mixed coniferous and broad-leaved forests.   Method  A mixed coniferous and broad-leaved forest in Mount Tianmu was taken as the research object. Spearman’s correlation analysis was conducted between forest regeneration factors, soil factors and terrain factors. One-way ANOVA was used to analyze the differences in forest regeneration among different soil and terrain factors. A linear regression model was constructed to quantitatively describe the relationship between forest regeneration and terrain and soil factors.   Result  (1) There were 79 species of mixed coniferous and broad-leaved forests in Mount Tianmu, among which the dominant species were Camellia fraterna, Cyclobalanopsis glauca, Lithocarpus brevicaudatus, Cyclobalanopsis myrsinifolia, Machilus pingii, Rhododendron ovatum, Torreya grandis, Lindera glauca, Eurya japonica, and Litsea coreana. (2) There was a highly significant negative correlation between available potassium and the total number of updated tree species (P<0.01), a highly significant positive correlation between altitude and the total number of updated tree species (P<0.01), a significant negative correlation between organic matter and the number of updated tree species (P<0.05), and a highly significant positive correlation between available potassium and the total number of updated tree species (P<0.01). (3) There was a significant difference in the total number of updated tree species with different slope orientations and available potassium levels (P<0.05). There were significant differences in the number of updated tree species with different grades of slope and soil organic matter (P<0.05). (4) In the linear regression model with the total number of upgraded tree species as the dependent variable, the regression coefficients of available potassium and altitude were significant (P<0.05).   Conclusion  In the mixed coniferous and broad-leaved forests in Mount Tianmu, altitude, slope, aspect, soil organic matter and available potassium have significant effects on forest regeneration. [Ch, 3 fig. 5 tab. 49 ref.]
Molecular regulatory mechanisms of cambium activity in woody plants
YE Qingqing, ZHOU Mingbing
, Available online  doi: 10.11833/j.issn.2095-0756.20230473
Abstract:
The vascular cambium is located between the xylem and phloem, which is responsible for many growth and development processes, and also plays an important role in wood production. In simulating cambium growth and potential molecular processes, deep imaging combined with gene expression analysis is a cutting-edge research direction, and mathematical modeling and simulation combined with real-time imaging have important application prospects. Based on this technique, some representative achievements at the molecular level of cambium activity are summarized, and the future research prospects are proposed. At present, molecular studies on cambium activity in plants mainly focus on: (1) cambium activity is regulated by plant hormone signaling; (2) cambium activity is regulated by transcription factors and peptide receptor signaling; (3) cambium activity is regulated by receptor kinase signals peptide receptor signaling. The main conclusions are that WOX4, WOX14, HB4, HB7, HB8 and ANT positively regulate the activities of tree cambium and can be used as the first choice for transgenic wood roughening breeding. In the future, the analysis of cell-to-cell communication connections in the cambium through computer models can better analyze the molecular mechanism of vascular cambium development in woody plants. [Ch. 3 tab. 68 ref.]
Simulation of micro-droplet injection process and key parameters based on 3DP equipment of wood-plastic composites
LIU Zixin, WANG Qi, YAN Chenglin, WANG Xinxu, LI Xiaoxu
, Available online  doi: 10.11833/j.issn.2095-0756.20230511
Abstract:
  Objective  Based on the self-developed wood-plastic composite material 3DP equipment micro-droplet injection system, research on the process of UV adhesive micro-droplet injection is aimed to optimize the processing parameters and the structural parameters of the micro-droplet injection system, and provide data support for the study of droplet spreading and infiltration in the 3DP process of wood plastic composite materials.  Method  The displacement characteristics of the needle-type jet valve and the rheological properties of the UV adhesive were analyzed to determine the injection parameters affecting the micro-droplet injection process of the wood plastic composite 3DP processing. The finite element method was used to simulate the micro-droplet injection process to analyze the influence of injection parameters and obtain the variation characteristics of droplet velocity, pressure and mass flow rate at the nozzle during the micro-droplet injection process. The needle velocity, nozzle diameter and UV adhesive supply pressure as evaluation factors, and the mass of the droplet and the main droplet velocity as indicators, simulation experiments were conducted to optimize parameter combination.   Result  The results of the single factor experiment indicated that needle speed 0.3−0.9 m·s−1, nozzle diameter 0.10−0.20 mm, UV adhesive supply pressure 0.1−0.3 MPa were reasonable printing parameters. The results of the orthogonal experiment indicated that nozzle diameter is a significant factor of droplet mass, and the UV adhesive supply pressure is a significant factor of the droplet velocity. The droplet mass and droplet velocity are good when the nozzle diameter is 0.10 mm, the needle speed is 0.9 m·s−1, the UV adhesive supply pressure is 0.1 MPa, and the valve seat cone angle is 130°.   Conclusion  The VOF finite element model of 3DP micro-droplet injection process of wood-plastic composite was established on the basis of pre-experiment and theoretical analysis. The study on the influence of injection parameters on micro-droplet injection has been completed, and the optimal combination of injection parameters has been obtained. [Ch, 8 fig. 4 tab. 17 ref.]
Effect of foliar fertilization on seed quality of Torreya grandis ‘Merrillii’ during seed filling period
HAO Qichun, XIE Jiquan, DAI Wensheng, LI Keyu, YU Chenliang, YU Weiwu
, Available online  doi: 10.11833/j.issn.2095-0756.20230194
[Abstract](147) [HTML] (50) [PDF](21)
Abstract:
  Objective  This study aims to explore aspects of Torreya grandis ‘Merrillii’ seeds, including appearance, nutritional quality, element content, flower bud differentiation, and expansion and fruit setting, so as to provide a theoretical basis for supplementing tree nutrition and improving seed quality.   Method  During the seed filling period of T. grandis ‘Merrillii’ from June to August, the seeds were treated with water as a control and sprayed with 7 commercial foliar fertilizers (amino acid water-soluble fertilizer, fulvic acid water-soluble fertilizer, active potassium water-soluble fertilizer, high-strength calcium water-soluble fertilizer, liquid boron water-soluble fertilizer, trace element water-soluble fertilizer, and macro-element water-soluble fertilizer). Physiological indicators of seeds in terms of seed morphology, oil content and fatty acid composition, crude protein content, starch content, soluble sugar content, and flowering intensity were measured and their effects on seed quality were analyzed.   Result  Spraying amino acid water-soluble fertilizer and macro-element water-soluble fertilizer significantly increased (P<0.05) the kernel shape index, single kernel mass, nucleation rate, kernel type index, and single kernel mass of seeds, as well as oil content, soluble sugar content, and flowering intensity, and significantly reduced starch content. Among them, the single kernel mass of seeds treated with amino acid water-soluble fertilizer increased by 9.8% compared with the control, while the starch content decreased by 1.9%. The single kernel mass, oil content, and soluble sugar content of seeds treated with macro-element water-soluble fertilizer increased by 9.5%, 11.9%, and 15.9%, respectively, compared with the control. At the same time, spraying amino acid water-soluble fertilizer significantly promoted (P<0.05) the content of taxoleic acid in seeds, which increased by 15.9% compared with the control, while spraying macro-element water-soluble fertilizer significantly (P<0.05) increased the content of unsaturated fatty acids in seeds by 4.2% compared with the control. In addition, high-strength calcium water-soluble fertilizer had a significant promoting effect (P<0.05) on the increase of kernel yield, flowering intensity, and fruit setting rate, which were 3.3%, 17.1%, and 10.9% higher than the control. The active potassium water-soluble fertilizer significantly increased the protein content of seeds by 13.6% compared with the control. Principal component analysis showed that the comprehensive quality score of seeds treated with amino acid water-soluble fertilizer was the highest.   Conclusion  The amino acid water-soluble fertilizer treatment has the best effect, followed by high-strength calcium water-soluble fertilizer and macro-element water-soluble fertilizer. [Ch. 3 fig. 5 tab. 29 ref.]