不同植物生长调节物质对条叶榕组织培养的影响

周燕青; 丁兰; 徐步青; 夏国华; 崔永一

doi:10.11833/j.issn.2095-0756.2013.03.024

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不同植物生长调节物质对条叶榕组织培养的影响

周燕青 , 丁兰 , 徐步青 , 夏国华 , 崔永一

文章导航 > 浙江农林大学学报 > 2013 > 30(3): 453-458

周燕青, 丁兰, 徐步青, 夏国华, 崔永一. 不同植物生长调节物质对条叶榕组织培养的影响[J]. 浙江农林大学学报, 2013, 30(3): 453-458. doi: 10.11833/j.issn.2095-0756.2013.03.024

引用本文:

ZHOU Yanqing, DING Lan, XU Buqing, XIA Guohua, CUI Yongyi. Tissue culture and rapid propagation of Ficus pandurata var.angustifolia with different plant growth regulators[J]. Journal of Zhejiang A&F University, 2013, 30(3): 453-458. doi: 10.11833/j.issn.2095-0756.2013.03.024

Citation:

ZHOU Yanqing, DING Lan, XU Buqing, XIA Guohua, CUI Yongyi. Tissue culture and rapid propagation of Ficus pandurata var.angustifolia with different plant growth regulators[J]. Journal of Zhejiang A&F University, 2013, 30(3): 453-458. doi: 10.11833/j.issn.2095-0756.2013.03.024

不同植物生长调节物质对条叶榕组织培养的影响

doi: 10.11833/j.issn.2095-0756.2013.03.024

周燕青^1,2,
丁兰³,
徐步青^2,4,
夏国华^5, ,,
崔永一¹

1.
浙江农林大学农业与食品科学学院，浙江临安 311300;
2.
临安成蹊农业科技开发有限公司，浙江临安 311300;
3.
浙江省临安市农业技术推广中心，浙江临安 311300;
4.
浙江农林大学风景园林与建筑学院，浙江临安 311300;
5.
浙江农林大学林业与生物技术学院，浙江临安 311300

详细信息

通信作者: 夏国华

Tissue culture and rapid propagation of Ficus pandurata var.angustifolia with different plant growth regulators

1.
School of Agriculture and Food Science, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;
2.
Lin’an Seikei Agricultural Science and Technology Development Co., Ltd, Lin’an 311300, Zhejiang, China;
3.
Lin’an Agricultural Technology Promotion Center, Lin’an 311300, Zhejiang, China;
4.
School of Landscape Architecture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;
5.
School of Forestry and Biotechnology, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

More Information

Corresponding author: XIA Guohua

摘要: 为了更好地开发利用条叶榕Ficus pandurata var. angustifolia这一畲族习用药用植物，以条叶榕无菌植株为外植体，对影响条叶榕叶片愈合组织诱导、不定芽分化、增殖和壮苗生根的主导因子植物生长调节物质进行了研究。结果表明：叶片在添加不同植物生长调节剂物质（包括6-苄基腺嘌呤6-BA，2,4-二氯苯氧乙酸2,4-D，萘乙酸NAA）和不同质量浓度的培养基中均能诱导愈合组织，且诱导率均达到100%，但愈合组织进一步分化成不定芽较为困难，以MS+1.0 mgL－16-BA +0.2 mgL－12,4-D +0.1 mgL－1NAA分化率最高，为6.67%；不定芽在添加不同植物生长调节物质种类（苯基噻二唑基脲TDZ，6-BA，NAA）和不同质量浓度的培养基中均表现出较好的增殖效果，3种植物生长调节物质对不定芽增殖的作用大小依次为6-BA，TDZ和NAA，以MS+0.3 mgL－1TDZ +1.0 mgL－16-BA +0.3 mgL－1NAA增殖倍数最高，达到8.93；条叶榕生根容易，生根率均达到100%。图1表4参13
- 植物学 /
- 条叶榕 /
- 愈合组织诱导 /
- 不定芽增殖 /
- 生根
Abstract: Little information on callus induction, proliferation and regeneration from leaf explants of Ficus pandurata var. angustifolia, a commonly used medication with the She ethnic minority, has been found. To develop and utilize this ethnic herb, an in vitro induction and shoot regeneration from leaf explants was evaluated using an orthogonal experimental design with plant growth regulators，including 6-Benzylaminopurine（6-BA）, 2,4-dichlorophenoxyacetic acid（2,4-D）, Thidiazuron（TDZ）, and naphthaleneacetic acid（NAA）， concentrations in a Murashige and Skoog（MS） media. Results showed that callus induction ratio in all 9 treatments exceeded 100%, but adventitious buds were extremely difficult to differentiate. The optimum medium for adventitious buds differentiation was MS + 1.0 mgL－1 6-BA + 0.2 mgL－1 2,4-D + 0.1 mgL－1 NAA, the differentiation rate of adventitious buds reached 6.7%. The orthogonal experimental design showed that the proliferation capacity of plant growth regulators was in the order: 6-BA ＞ TDZ ＞NAA. The optimum medium for proliferation was MS + 0.3 mgL－1 TDZ + 1.0 mgL－1 6-BA + 0.3 mgL－1 NAA, the multiplication rate was 8.93, and the rooting rate in all 9 treatments was 100%. The tissue culture technique and rapid propagation system of Ficus pandurata var. angustifolia could be used for large-scale seedlings in short time and provide technical guidance for large-scale production.［Ch, 1 tab. 4 tab. 13 ref.］
- botany /
- Ficus pandurata var. angustifolia /
- callus induction /
- differentiation /
- rooting

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不同植物生长调节物质对条叶榕组织培养的影响

doi: 10.11833/j.issn.2095-0756.2013.03.024

1. 浙江农林大学农业与食品科学学院，浙江临安 311300;

2. 临安成蹊农业科技开发有限公司，浙江临安 311300;

3. 浙江省临安市农业技术推广中心，浙江临安 311300;

4. 浙江农林大学风景园林与建筑学院，浙江临安 311300;

5. 浙江农林大学林业与生物技术学院，浙江临安 311300

通信作者: 夏国华

收稿日期: 2012-06-04
修回日期: 2012-07-26
刊出日期: 2013-06-20

关键词:

摘要: 为了更好地开发利用条叶榕Ficus pandurata var. angustifolia这一畲族习用药用植物，以条叶榕无菌植株为外植体，对影响条叶榕叶片愈合组织诱导、不定芽分化、增殖和壮苗生根的主导因子植物生长调节物质进行了研究。结果表明：叶片在添加不同植物生长调节剂物质（包括6-苄基腺嘌呤6-BA，2,4-二氯苯氧乙酸2,4-D，萘乙酸NAA）和不同质量浓度的培养基中均能诱导愈合组织，且诱导率均达到100%，但愈合组织进一步分化成不定芽较为困难，以MS+1.0 mgL－16-BA +0.2 mgL－12,4-D +0.1 mgL－1NAA分化率最高，为6.67%；不定芽在添加不同植物生长调节物质种类（苯基噻二唑基脲TDZ，6-BA，NAA）和不同质量浓度的培养基中均表现出较好的增殖效果，3种植物生长调节物质对不定芽增殖的作用大小依次为6-BA，TDZ和NAA，以MS+0.3 mgL－1TDZ +1.0 mgL－16-BA +0.3 mgL－1NAA增殖倍数最高，达到8.93；条叶榕生根容易，生根率均达到100%。图1表4参13

English Abstract

Tissue culture and rapid propagation of Ficus pandurata var.angustifolia with different plant growth regulators

1. School of Agriculture and Food Science, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;

2. Lin’an Seikei Agricultural Science and Technology Development Co., Ltd, Lin’an 311300, Zhejiang, China;

3. Lin’an Agricultural Technology Promotion Center, Lin’an 311300, Zhejiang, China;

4. School of Landscape Architecture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;

5. School of Forestry and Biotechnology, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Corresponding author: XIA Guohua

Received Date: 2012-06-04
Rev Recd Date: 2012-07-26
Publish Date: 2013-06-20

Keywords:

Abstract: Little information on callus induction, proliferation and regeneration from leaf explants of Ficus pandurata var. angustifolia, a commonly used medication with the She ethnic minority, has been found. To develop and utilize this ethnic herb, an in vitro induction and shoot regeneration from leaf explants was evaluated using an orthogonal experimental design with plant growth regulators，including 6-Benzylaminopurine（6-BA）, 2,4-dichlorophenoxyacetic acid（2,4-D）, Thidiazuron（TDZ）, and naphthaleneacetic acid（NAA）， concentrations in a Murashige and Skoog（MS） media. Results showed that callus induction ratio in all 9 treatments exceeded 100%, but adventitious buds were extremely difficult to differentiate. The optimum medium for adventitious buds differentiation was MS + 1.0 mgL－1 6-BA + 0.2 mgL－1 2,4-D + 0.1 mgL－1 NAA, the differentiation rate of adventitious buds reached 6.7%. The orthogonal experimental design showed that the proliferation capacity of plant growth regulators was in the order: 6-BA ＞ TDZ ＞NAA. The optimum medium for proliferation was MS + 0.3 mgL－1 TDZ + 1.0 mgL－1 6-BA + 0.3 mgL－1 NAA, the multiplication rate was 8.93, and the rooting rate in all 9 treatments was 100%. The tissue culture technique and rapid propagation system of Ficus pandurata var. angustifolia could be used for large-scale seedlings in short time and provide technical guidance for large-scale production.［Ch, 1 tab. 4 tab. 13 ref.］

引用本文: