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摘要: 分析施硅对干旱胁迫下雷竹Phyllostachys violascens生理生化性状的影响,探讨施硅对雷竹抗旱性的影响机制。采用水培实验的方法,以聚乙二醇(PEG 6000)模拟干旱胁迫,外加不同浓度的硅,测定它们对雷竹叶片可溶性糖质量分数、脯氨酸质量分数、过氧化物酶活性、过氧化氢酶活性及光合参数等指标的影响。研究表明:干旱胁迫下,与对照相比,不施硅处理雷竹叶片可溶性糖、脯氨酸质量分数及过氧化物酶活性分别上升至487.89 molg-1,57.52 gg-1和178.125 molg-1min-1分别高出对照59.00%,38.47%和32.56%,过氧化氢酶活性下降至56.11 molg-1min-1,低于对照18.3%,光合作用显著受到抑制。施硅处理雷竹叶片可溶性糖、脯氨酸及过氧化物酶活性较不施硅处理雷竹叶片的显著降低,与处理2(PEG 6000)相比,处理3[PEG 6000+1.8 mmolL-1 Si(二氧化硅)]分别降低了50.31%,35.94%和50.88%;处理4[PEG 6000+5.4 mmolL-1 Si]分别降低了43.69%,44.77%和68.42%。同时,过氧化氢酶活性显著上升,与处理2(PEG 6000)相比,处理3上升21.91%;处理4上升25.87%,光合参数显著增强。干旱胁迫条件下施硅处理可以提高雷竹的抗旱性。图4表1参26Abstract: To clarify how silicon(Si) enhances resistance to drought stress,the effects of exogenous Si on physiological and biochemical characteristics,including soluble sugar content,proline (Pro) content,peroxidase(POD) activity,catalase(CAT) activity,and photosynthetic characteristics,of Lei bamboo (Phyllostachys violascens) were determined with drought stress induced by polyethylene glycol (PEG6000) in hydroponic conditions. Treatments were as follows:(1)Control,(2)PEG 6000,(3)PEG 6000 + 1.8 mmolL-1 Si,and (4)PEG 6000 + 5.4 mmolL-1 Si. Results showed that with drought stress, compared to the treatment 1,soluble sugar content increased to 487.89 molg-1(59.00%)(P<0.05),proline content to 57.52 gg-1 (38.47%)(P<0.05),and POD activity of treatment 2 to 178.125 nmol mg-1min-1(32.56%)(P<0.05),but CAT activity decreased to 56.11 molg-1min-1(18.30%)(P<0.05),and leaf photosynthesis was inhibited. With treatment 3 compared to treatment 2,exogenous Si decreased soluble sugar content (50.31%)(P<0.05),proline content (35.94%)(P<0.05),and POD activity (50.88%)(P<0.05);and with treatment 4 compared to treatment 2,exogenous Si decreased soluble sugar content(43.69%)(P<0.05),proline content (44.77%)(P<0.05),and POD activity (68.42%)(P<0.05). However, treatment 3 with exogenous Si increased CAT activity 21.9% more than treatment 2(P<0.05);and treatment 4 was 25.87% more than treatment 2(P<0.05). Exogenous Si also enhanced leaf photosynthesis. This indicated that Si could increase drought resistance of Lei bamboo.[Ch,4 fig. 1 tab. 26 ref.]
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Key words:
- botany /
- silicon /
- Phyllostachys violascens /
- drought stress /
- physiological and biochemical indexes /
- PEG 6000
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2013.06.008
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