Cloning and expression analysis of a flowering-related gene，<em>OAP</em> 3，in <em>Oncidium</em>

XU Xiao-yan; TIAN Min; WANG Cai-xia; LONG Ming-hua

doi:10.11833/j.issn.2095-0756.2011.06.010

Volume 28 Issue 6

Dec. 2011

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XU Xiao-yan, TIAN Min, WANG Cai-xia, LONG Ming-hua. Cloning and expression analysis of a flowering-related gene，OAP 3，in Oncidium[J]. Journal of Zhejiang A&F University, 2011, 28(6): 900-906. doi: 10.11833/j.issn.2095-0756.2011.06.010

Citation:

XU Xiao-yan, TIAN Min, WANG Cai-xia, LONG Ming-hua. Cloning and expression analysis of a flowering-related gene，OAP 3，in Oncidium[J]. Journal of Zhejiang A&F University, 2011, 28(6): 900-906. doi: 10.11833/j.issn.2095-0756.2011.06.010

Cloning and expression analysis of a flowering-related gene，OAP 3，in Oncidium

doi: 10.11833/j.issn.2095-0756.2011.06.010

1.
Research Institute of Subtropical Forestry，Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China;
2.
Agricultural College，Guangxi University，Nanning 530003，Guangxi，China

More Information

Corresponding author: TIAN Min
Publish Date: 2011-12-20

Abstract

To investigate the expression pattern of MADS-box gene during the development of the orchids flower，the floral organ identity gene OAP 3 with a MADS-box motif was cloned from an orchid plant through the RT-PCR，OncidiumMilliongolds（GenBank accession No.GU644447）. Then a sequence analysis and a phylogenetic analysis were conducted along with a quantitative real-time polymerase chain reaction （qRT-PCR）. The full length cDNA of OAP 3 was 799 bp with an open reading frame（ORF） encoding 204 amino acids. The deduced amino acids sequence contained a typical MADS domain and a K domain，and the sequence analysis indicated that OAP 3 shared 50%-78% homology in the amino acid sequence with Arabidopsis thaliana and other species of APETALA3 homologues. The phylogenetic analysis suggested that OAP 3 belonged to the B gene，but four consensus motifs identified in the C-terminal region of the B group genes were absent in the OAP 3 gene. The qRT-PCR analysis showed that OAP 3 mRNA increased with flower bud development，as well as in all four floral organs，vegetative leaves，and shoots.［Ch，5 fig. 1 tab. 17 ref.］
- molecular biology,
- Oncidium‘Milliongolds’,
- floral initiation,
- MADS-box genes,
- OAP 3 gene,
- qRT-PCR

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Cloning and expression analysis of a flowering-related gene，OAP 3，in Oncidium

1. Research Institute of Subtropical Forestry，Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China;

2. Agricultural College，Guangxi University，Nanning 530003，Guangxi，China

Corresponding author: TIAN Min

Publish Date: 2011-12-20

Key words:

Abstract: To investigate the expression pattern of MADS-box gene during the development of the orchids flower，the floral organ identity gene OAP 3 with a MADS-box motif was cloned from an orchid plant through the RT-PCR，OncidiumMilliongolds（GenBank accession No.GU644447）. Then a sequence analysis and a phylogenetic analysis were conducted along with a quantitative real-time polymerase chain reaction （qRT-PCR）. The full length cDNA of OAP 3 was 799 bp with an open reading frame（ORF） encoding 204 amino acids. The deduced amino acids sequence contained a typical MADS domain and a K domain，and the sequence analysis indicated that OAP 3 shared 50%-78% homology in the amino acid sequence with Arabidopsis thaliana and other species of APETALA3 homologues. The phylogenetic analysis suggested that OAP 3 belonged to the B gene，but four consensus motifs identified in the C-terminal region of the B group genes were absent in the OAP 3 gene. The qRT-PCR analysis showed that OAP 3 mRNA increased with flower bud development，as well as in all four floral organs，vegetative leaves，and shoots.［Ch，5 fig. 1 tab. 17 ref.］

Cloning and expression analysis of a flowering-related gene，OAP 3，in Oncidium

doi: 10.11833/j.issn.2095-0756.2011.06.010

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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Cloning and expression analysis of a flowering-related gene，OAP 3，in Oncidium

doi: 10.11833/j.issn.2095-0756.2011.06.010

1. Research Institute of Subtropical Forestry，Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China;

2. Agricultural College，Guangxi University，Nanning 530003，Guangxi，China

Corresponding author: TIAN Min

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Cloning and expression analysis of a flowering-related gene，OAP 3，in Oncidium

doi: 10.11833/j.issn.2095-0756.2011.06.010

Abstract

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通讯作者: 陈斌, bchen63@163.com

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Cloning and expression analysis of a flowering-related gene，OAP 3，in Oncidium

doi: 10.11833/j.issn.2095-0756.2011.06.010

1. Research Institute of Subtropical Forestry，Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China; 2. Agricultural College，Guangxi University，Nanning 530003，Guangxi，China

Corresponding author: TIAN Min

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1. Research Institute of Subtropical Forestry，Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China;

2. Agricultural College，Guangxi University，Nanning 530003，Guangxi，China