Selection of strains used to degrade organic mulching materials from <em>Phyllostachys violascens</em> forest and optimization of its enzyme production

KE Xiao; CHEN Shuang-lin; ZHANG Xiao-ping; GUO Zi-wu

doi:10.11833/j.issn.2095-0756.2012.02.014

Volume 29 Issue 2

Mar. 2012

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KE Xiao, CHEN Shuang-lin, ZHANG Xiao-ping, GUO Zi-wu. Selection of strains used to degrade organic mulching materials from Phyllostachys violascens forest and optimization of its enzyme production[J]. Journal of Zhejiang A&F University, 2012, 29(2): 244-250. doi: 10.11833/j.issn.2095-0756.2012.02.014

Citation:

KE Xiao, CHEN Shuang-lin, ZHANG Xiao-ping, GUO Zi-wu. Selection of strains used to degrade organic mulching materials from Phyllostachys violascens forest and optimization of its enzyme production[J]. Journal of Zhejiang A&F University, 2012, 29(2): 244-250. doi: 10.11833/j.issn.2095-0756.2012.02.014

Selection of strains used to degrade organic mulching materials from Phyllostachys violascens forest and optimization of its enzyme production

doi: 10.11833/j.issn.2095-0756.2012.02.014

1.
Research Institute of Subtropical Forestry，Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China;
2.
College of Resources and Environment，Sichuan Agricultural University，Ya’an 625014，Sichuan，China

More Information

Corresponding author: GUO Zi-wu1
Received Date: 2011-07-07
Rev Recd Date: 2011-09-29
Publish Date: 2012-04-20

Abstract

Severe degradation of a Phyllostachys violascens forest has occurred from organic mulch residues for years. To artificially promote decomposition of organic mulching material and promote regeneration of bamboo forest，seven cellulase-producing strains，including 4 fungi and 3 actinomycetes，from organic matter and soil of a Ph. violascens forest were chosen using a Congo red cellulose identification culture medium. A filter paper enzyme activity （FPA） as well as colony morphology and biological characteristics were used for the analysis. Results showed that among all selected strains，the FPA of Penicillium spp. （Strain 2.1） was highest. Optimal fermentation conditions were as follows: an initial pH 5.0，a cultivation temperature of 30 ℃，and a fermentation time of 5 d. The optimal cultivation medium for Strain 2.1 was chosen as：straw powder （25.00 gL－1） for the C source，beef extract （2.50 gL－1） for the N source，and FeCl3 （0.01 gL－1） and KH2PO4 （1.50 gL－1） for important inorganic salts.［Ch，4 fig. 3 tab. 20 ref.］
- microbiology,
- Phyllostachys violascens,
- organic mulching material,
- decomposing strain,
- cellulase,
- enzyme production

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Selection of strains used to degrade organic mulching materials from Phyllostachys violascens forest and optimization of its enzyme production

1. Research Institute of Subtropical Forestry，Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China;

2. College of Resources and Environment，Sichuan Agricultural University，Ya’an 625014，Sichuan，China

Corresponding author: GUO Zi-wu1

Received Date: 2011-07-07

Rev Recd Date: 2011-09-29

Publish Date: 2012-04-20

Key words:

Abstract: Severe degradation of a Phyllostachys violascens forest has occurred from organic mulch residues for years. To artificially promote decomposition of organic mulching material and promote regeneration of bamboo forest，seven cellulase-producing strains，including 4 fungi and 3 actinomycetes，from organic matter and soil of a Ph. violascens forest were chosen using a Congo red cellulose identification culture medium. A filter paper enzyme activity （FPA） as well as colony morphology and biological characteristics were used for the analysis. Results showed that among all selected strains，the FPA of Penicillium spp. （Strain 2.1） was highest. Optimal fermentation conditions were as follows: an initial pH 5.0，a cultivation temperature of 30 ℃，and a fermentation time of 5 d. The optimal cultivation medium for Strain 2.1 was chosen as：straw powder （25.00 gL－1） for the C source，beef extract （2.50 gL－1） for the N source，and FeCl3 （0.01 gL－1） and KH2PO4 （1.50 gL－1） for important inorganic salts.［Ch，4 fig. 3 tab. 20 ref.］

Selection of strains used to degrade organic mulching materials from Phyllostachys violascens forest and optimization of its enzyme production

doi: 10.11833/j.issn.2095-0756.2012.02.014

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Selection of strains used to degrade organic mulching materials from Phyllostachys violascens forest and optimization of its enzyme production

doi: 10.11833/j.issn.2095-0756.2012.02.014

1. Research Institute of Subtropical Forestry，Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China;

2. College of Resources and Environment，Sichuan Agricultural University，Ya’an 625014，Sichuan，China

Corresponding author: GUO Zi-wu1

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Selection of strains used to degrade organic mulching materials from Phyllostachys violascens forest and optimization of its enzyme production

doi: 10.11833/j.issn.2095-0756.2012.02.014

Abstract

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References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Selection of strains used to degrade organic mulching materials from Phyllostachys violascens forest and optimization of its enzyme production

doi: 10.11833/j.issn.2095-0756.2012.02.014

1. Research Institute of Subtropical Forestry，Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China; 2. College of Resources and Environment，Sichuan Agricultural University，Ya’an 625014，Sichuan，China

Corresponding author: GUO Zi-wu1

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1. Research Institute of Subtropical Forestry，Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China;

2. College of Resources and Environment，Sichuan Agricultural University，Ya’an 625014，Sichuan，China