Molecular identification of the strain ZNLD-18 selectively decomposing bamboo lignin

ZHOU Guo-ying; LI He

Volume 25 Issue 4

Aug. 2008

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ZHOU Guo-ying, LI He. Molecular identification of the strain ZNLD-18 selectively decomposing bamboo lignin[J]. Journal of Zhejiang A&F University, 2008, 25(4): 497-501.

Citation:

ZHOU Guo-ying, LI He. Molecular identification of the strain ZNLD-18 selectively decomposing bamboo lignin[J]. Journal of Zhejiang A&F University, 2008, 25(4): 497-501.

Molecular identification of the strain ZNLD-18 selectively decomposing bamboo lignin

1.
College of Resources and Environment，Central South University of Forestry and Technology，Changsha 410004，Hunan，China

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Corresponding author: ZHOU Guo-ying
Publish Date: 2008-08-20

Abstract

This objective was to provide basis for the biological extracting of the spinning bamboo fiber. The strain ZNLD-18，separated from decaying bamboo，which was able to decompose selectively the bamboo lignin，was identified by polymerase chain reaction （PCR）. The rDNA ITS sequences of the strain were amplified and tested by the fungi universal primers ITS1 and ITS4. The sequence length was 541 base pair（bp）. The sequences were Blast searched within the GenBank to obtain the different strains’ sequences with the similar homology. The comparison of the genetic distances of these sequences showed that the ITS sequences of ZNLD-18 and Trametes versicolor were with over 99% homology. A molecular phylogenetic tree was built with Phylogenetic Analysis Using Parsimony （PAUP） software. Given the analysis results of the tree and the morphological characteristics of the strain，ZNLD-18 was Trametes versicolor. ［Ch，2 fig. 2 tab. 14 ref.］
- microbiology,
- bamboo wood,
- lignin,
- selectively decomposing,
- fungi strain,
- rDNA ITS,
- molecular identification

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Molecular identification of the strain ZNLD-18 selectively decomposing bamboo lignin

1. College of Resources and Environment，Central South University of Forestry and Technology，Changsha 410004，Hunan，China

Corresponding author: ZHOU Guo-ying

Publish Date: 2008-08-20

Key words:

Abstract: This objective was to provide basis for the biological extracting of the spinning bamboo fiber. The strain ZNLD-18，separated from decaying bamboo，which was able to decompose selectively the bamboo lignin，was identified by polymerase chain reaction （PCR）. The rDNA ITS sequences of the strain were amplified and tested by the fungi universal primers ITS1 and ITS4. The sequence length was 541 base pair（bp）. The sequences were Blast searched within the GenBank to obtain the different strains’ sequences with the similar homology. The comparison of the genetic distances of these sequences showed that the ITS sequences of ZNLD-18 and Trametes versicolor were with over 99% homology. A molecular phylogenetic tree was built with Phylogenetic Analysis Using Parsimony （PAUP） software. Given the analysis results of the tree and the morphological characteristics of the strain，ZNLD-18 was Trametes versicolor. ［Ch，2 fig. 2 tab. 14 ref.］

Molecular identification of the strain ZNLD-18 selectively decomposing bamboo lignin

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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