低温引发处理过程中三尖杉种子生理变化及产地差异

焦月玲; 周志春; 金国庆; 李因刚

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

低温引发处理过程中三尖杉种子生理变化及产地差异

焦月玲 , 周志春 , 金国庆 , 李因刚

文章导航 > 浙江农林大学学报 > 2007 > 24(2): 173-178

焦月玲, 周志春, 金国庆, 李因刚. 低温引发处理过程中三尖杉种子生理变化及产地差异[J]. 浙江农林大学学报, 2007, 24(2): 173-178.

引用本文:

焦月玲, 周志春, 金国庆, 李因刚. 低温引发处理过程中三尖杉种子生理变化及产地差异[J]. 浙江农林大学学报, 2007, 24(2): 173-178.

JIAO Yue-ling, ZHOUZhi-chun, JINGuo-qing, LI Yin-gang. Cephalotaxus fortunei seed-physiological changes and differences among three seed sources during low temperature priming[J]. Journal of Zhejiang A&F University, 2007, 24(2): 173-178.

Citation:

JIAO Yue-ling, ZHOUZhi-chun, JINGuo-qing, LI Yin-gang. Cephalotaxus fortunei seed-physiological changes and differences among three seed sources during low temperature priming[J]. Journal of Zhejiang A&F University, 2007, 24(2): 173-178.

低温引发处理过程中三尖杉种子生理变化及产地差异

1.
中国林业科学研究院亚热带林业研究所,浙江富阳311400

基金项目:

浙江省科学技术攻关项目(2005C32035)

详细信息

作者简介: 焦月玲,硕士,从事林木种苗技术研究。

通信作者: 周志春,研究员,博士,博士生导师,从事亚热带重要针阔叶用材树种育种和栽培技术等研究。

中图分类号: S718.43

Cephalotaxus fortunei seed-physiological changes and differences among three seed sources during low temperature priming

1.
Research Institute of Subtropical Forestry, the Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China

Funds:

浙江省科学技术攻关项目(2005C32035)

摘要: 选用对低温引发处理反应差异显著的贵州黎平、福建明溪和四川盐源3个产地的三尖杉Cephalotaxus fortunei种子,设置不同的低温引发处理,研究它们在低温引发处理过程中的生理变化及产地差异。结果表明,经低温引发处理后上述3个产地种子发芽率差异巨大,分别为30.86%, 9.90%和1.91%,而在不同处理中则以10 d冷藏引发处理的种子发芽率较高。三尖杉种子在低温引发处理过程中代谢活跃,脂肪和淀粉质量分数不断下降,氨基酸质量分数大量增加,过氧化物酶(POD)和过氧化氢酶(CAT)活性显著增强。低温引发处理效果较好的福建明溪产地种子,其胚乳脱落酸(ABA)质量分数在处理前较高,处理后则显著降低,赤霉酸(GA3)/ABA变化较小或略有提高,这可能是解除该产地种子休眠的原因之一。四川盐源三尖杉种子在低温引发处理前胚乳ABA质量分数很低、GA3/ABA较高,但不能发芽,而在低温引发处理后种子胚乳ABA质量分数保持不变或有较大幅度的升高,说明另有原因控制其种子的休眠。四川盐源三尖杉种子对低温引发处理反应较小,应与它长期适应冬暖气候有关。此外,福建明溪三尖杉种子直接沙藏种子发芽率较低似与种子胚乳ABA水平较高有关。表5参8
- 植物学 /
- 三尖杉 /
- 种子 /
- 产地 /
- 解除休眠 /
- 低温引发处理 /
- 生理机制
Abstract: Cephalotaxus fortunei seeds from three seed sources, namely Liping,Guizhou Province;Mingxi,Fujian Province; and Yanyuan, Sichuan Province, were used to determine physiological changes during low temperature priming(direct sand storage in 5℃ for 10 days, 20 days or 30 days)as well as variation among seed sources by measuring fattiness, amylum, amino acid, POD(peroxidase), CAT(catalase), IAA(indoleacetic acid), GA3(gibberellin), ABA(abscisic acid) and ZR(zealin) concentrations in seeds. Results after low temperature priming indicated that the average germination rate of Liping seeds(30.9%) was greater than Mingxi seeds (9.9%)and Yanyuan seeds(1.9%). Of the different treatments, the 10 days low temperature priming had the highest seed germination rate. In addition, during low temperature priming, active metabolism, decreased levels of fat and starch, increased amino acids, and enhanced POD CAT activity were detected. In the endosperm, seeds fromMingxi possessed lower levels of ABA after low temperature treatmentwith no obvious change or little increase of GA3 ABA ratio.Thismightbe one reason for seed dormancy-breaking. In spite of a lowABA content and a high GA3 ABAratio in the endospermof Yanyuan seedsbefore lowtemperature priming,germination did notoccur;ABA in the seed endospermincreased mostly after lowtemperature priming.This indicated thatwith Yanyuan seeds some unknown factors controlled seed dormancy; also, the lack of germinationwith lowtemperature primingmightbe the result of a long adaptation to warmerwinters in Sichuan.Moreover, the low germination rate of seeds fromMingxi with direct sand storage seemed to be related to high ABA content in the endosperm.[Ch, 5 tab. 8 ref.]
- botany /
- Cephalotaxus fortunei /
- seed /
- seed source /
- dormancy-breaking /
- low temperature priming /
- physiological mechanism

[1]	高璐, 李香格, 祁亨年, 臧影, 贾良权, 赵光武, 唐琦哲, 郑雯. 种子呼吸检测方法及其应用研究进展 . 浙江农林大学学报, 2022, 39(5): 1133-1143. doi: 10.11833/j.issn.2095-0756.20210748
[2]	尹焕焕, 刘青华, 周志春, 万雪琴, 余启新, 丰忠平. 马尾松无性系木材基本密度和纤维形态的变异及选择 . 浙江农林大学学报, 2020, 37(6): 1186-1192. doi: 10.11833/j.issn.2095-0756.20190720
[3]	唐继新, 雷渊才, 曾冀, 李忠国, 李武志, 农良书, 赵总. 米老排人工林皆伐迹地种子天然更新林密度调控效应 . 浙江农林大学学报, 2018, 35(3): 459-466. doi: 10.11833/j.issn.2095-0756.2018.03.009
[4]	陈志超, 黄婷婷, 王洋, 吴洪恺, 陈璋琦, 陶若芙. 水稻种子休眠特性与耐藏性的关系 . 浙江农林大学学报, 2018, 35(3): 490-496. doi: 10.11833/j.issn.2095-0756.2018.03.013
[5]	慎家辉, 秦安, 李甲明, 刘伦, 吴俊. 甲基磺酸乙酯诱变杜梨种子突变的鉴定与分析 . 浙江农林大学学报, 2014, 31(6): 892-897. doi: 10.11833/j.issn.2095-0756.2014.06.010
[6]	吴君, 李因刚, 罗修宝, 柳新红, 石从广, 何云核. 白花树种子生物学特性 . 浙江农林大学学报, 2014, 31(1): 9-13. doi: 10.11833/j.issn.2095-0756.2014.01.002
[7]	刘建强, 胡军飞, 欧丹燕, 胡冬冬, 金水虎. 厚藤种子萌发特性 . 浙江农林大学学报, 2011, 28(1): 153-157. doi: 10.11833/j.issn.2095-0756.2011.01.025
[8]	马惠芬, 郎南军, 和丽萍, 余珍, 郑科, 彭明俊, 向振勇, 孔继君, 袁瑞玲. 云南省建水县麻疯树不同部位挥发性化学成分 . 浙江农林大学学报, 2011, 28(4): 674-679. doi: 10.11833/j.issn.2095-0756.2011.04.024
[9]	张蕊, 王秀花, 章建红, 周志春. 3种冬青属植物种子解休眠过程中的生理变化 . 浙江农林大学学报, 2010, 27(4): 524-528. doi: 10.11833/j.issn.2095-0756.2010.04.008
[10]	李因刚, 周志春, 金国庆, 廖国华, 余能健, 王月生. 2年生三尖杉生长和2种酯碱种源变异及初步选择 . 浙江农林大学学报, 2008, 25(6): 749-755.
[11]	靳晓翠, 王伟, 刘玉艳. 天门冬种子萌发特性 . 浙江农林大学学报, 2007, 24(4): 428-432.
[12]	黎章矩, 骆成方, 程晓建, 冯肖军, 喻卫武. 香榧种子成分分析及营养评价 . 浙江农林大学学报, 2005, 22(5): 540-544.
[13]	孙鸿有, 郑勇平, 翁春媚, 罗小华, 涂武泰, 蔡克孝. 杉木种子园种子品质性状变异及遗传参数 . 浙江农林大学学报, 2005, 22(1): 61-65.
[14]	林霞, 陶正明, 张庆良, 黄建. 无柄小叶榕种子育苗技术 . 浙江农林大学学报, 2003, 20(3): 325-327.
[15]	黄坚钦, 何小春, 金水虎, 朱向东, 吴正晃. 夏蜡梅的双受精和种子形成 . 浙江农林大学学报, 1999, 16(3): 219-223.
[16]	史晓华, 黎念林, 金玲, 朱秋桂, 徐本美. 秤锤树种子休眠与萌发的初步研究^＊ . 浙江农林大学学报, 1999, 16(3): 228-233.
[17]	范义荣, 毛迎春, 方陆明, 余其龙, 奚鑫基, 王国英. 黄山松种子园营建技术及初步效果 . 浙江农林大学学报, 1997, 14(2): 111-119.
[18]	赖焕林, 王章荣. 马尾松无性系种子园花期花量分析 . 浙江农林大学学报, 1996, 13(4): 405-410.
[19]	金文通, 孙晓萍, 俞仲辂. 田间条件下雪松种子出苗规律 . 浙江农林大学学报, 1996, 13(3): 301-305.
[20]	樊后保, 臧润国. 模拟酸雨对樟树种子萌发和幼苗生长的影响` . 浙江农林大学学报, 1996, 13(4): 412-417.

链接本文:
https://zlxb.zafu.edu.cn/article/id/1258

https://zlxb.zafu.edu.cn/article/zjnldxxb/2007/2/173

点击查看大图

计量

文章访问数: 1499
HTML全文浏览量: 286
PDF下载量: 154
被引次数: 0

低温引发处理过程中三尖杉种子生理变化及产地差异

1. 中国林业科学研究院亚热带林业研究所,浙江富阳311400

基金项目:

浙江省科学技术攻关项目(2005C32035)

作者简介:
焦月玲,硕士,从事林木种苗技术研究。

通信作者: 周志春,研究员,博士,博士生导师,从事亚热带重要针阔叶用材树种育种和栽培技术等研究。

收稿日期: 2006-08-07
修回日期: 2006-10-31
刊出日期: 2007-04-20

中图分类号: S718.43

关键词:

摘要: 选用对低温引发处理反应差异显著的贵州黎平、福建明溪和四川盐源3个产地的三尖杉Cephalotaxus fortunei种子,设置不同的低温引发处理,研究它们在低温引发处理过程中的生理变化及产地差异。结果表明,经低温引发处理后上述3个产地种子发芽率差异巨大,分别为30.86%, 9.90%和1.91%,而在不同处理中则以10 d冷藏引发处理的种子发芽率较高。三尖杉种子在低温引发处理过程中代谢活跃,脂肪和淀粉质量分数不断下降,氨基酸质量分数大量增加,过氧化物酶(POD)和过氧化氢酶(CAT)活性显著增强。低温引发处理效果较好的福建明溪产地种子,其胚乳脱落酸(ABA)质量分数在处理前较高,处理后则显著降低,赤霉酸(GA3)/ABA变化较小或略有提高,这可能是解除该产地种子休眠的原因之一。四川盐源三尖杉种子在低温引发处理前胚乳ABA质量分数很低、GA3/ABA较高,但不能发芽,而在低温引发处理后种子胚乳ABA质量分数保持不变或有较大幅度的升高,说明另有原因控制其种子的休眠。四川盐源三尖杉种子对低温引发处理反应较小,应与它长期适应冬暖气候有关。此外,福建明溪三尖杉种子直接沙藏种子发芽率较低似与种子胚乳ABA水平较高有关。表5参8

English Abstract

Cephalotaxus fortunei seed-physiological changes and differences among three seed sources during low temperature priming

1. Research Institute of Subtropical Forestry, the Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China

Funds:

浙江省科学技术攻关项目(2005C32035)

Received Date: 2006-08-07
Rev Recd Date: 2006-10-31
Publish Date: 2007-04-20

Keywords:

Abstract: Cephalotaxus fortunei seeds from three seed sources, namely Liping,Guizhou Province;Mingxi,Fujian Province; and Yanyuan, Sichuan Province, were used to determine physiological changes during low temperature priming(direct sand storage in 5℃ for 10 days, 20 days or 30 days)as well as variation among seed sources by measuring fattiness, amylum, amino acid, POD(peroxidase), CAT(catalase), IAA(indoleacetic acid), GA3(gibberellin), ABA(abscisic acid) and ZR(zealin) concentrations in seeds. Results after low temperature priming indicated that the average germination rate of Liping seeds(30.9%) was greater than Mingxi seeds (9.9%)and Yanyuan seeds(1.9%). Of the different treatments, the 10 days low temperature priming had the highest seed germination rate. In addition, during low temperature priming, active metabolism, decreased levels of fat and starch, increased amino acids, and enhanced POD CAT activity were detected. In the endosperm, seeds fromMingxi possessed lower levels of ABA after low temperature treatmentwith no obvious change or little increase of GA3 ABA ratio.Thismightbe one reason for seed dormancy-breaking. In spite of a lowABA content and a high GA3 ABAratio in the endospermof Yanyuan seedsbefore lowtemperature priming,germination did notoccur;ABA in the seed endospermincreased mostly after lowtemperature priming.This indicated thatwith Yanyuan seeds some unknown factors controlled seed dormancy; also, the lack of germinationwith lowtemperature primingmightbe the result of a long adaptation to warmerwinters in Sichuan.Moreover, the low germination rate of seeds fromMingxi with direct sand storage seemed to be related to high ABA content in the endosperm.[Ch, 5 tab. 8 ref.]

焦月玲, 周志春, 金国庆, 李因刚. 低温引发处理过程中三尖杉种子生理变化及产地差异[J]. 浙江农林大学学报, 2007, 24(2): 173-178.

引用本文:

焦月玲, 周志春, 金国庆, 李因刚. 低温引发处理过程中三尖杉种子生理变化及产地差异[J]. 浙江农林大学学报, 2007, 24(2): 173-178.