山核桃外果皮提取物抑菌活性的初步研究

苏秀; 马良进; 陈安良; 张立钦

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

山核桃外果皮提取物抑菌活性的初步研究

苏秀 , 马良进 , 陈安良 , 张立钦

文章导航 > 浙江农林大学学报 > 2008 > 25(3): 355-358

苏秀, 马良进, 陈安良, 张立钦. 山核桃外果皮提取物抑菌活性的初步研究[J]. 浙江农林大学学报, 2008, 25(3): 355-358.

引用本文:

苏秀, 马良进, 陈安良, 张立钦. 山核桃外果皮提取物抑菌活性的初步研究[J]. 浙江农林大学学报, 2008, 25(3): 355-358.

SU Xiu, MA Liang-jin, CHEN An-liang, ZHANG Li-qin. Antifungal and antibacterial activity of extracts from the husk of Carya cathayensis[J]. Journal of Zhejiang A&F University, 2008, 25(3): 355-358.

Citation:

SU Xiu, MA Liang-jin, CHEN An-liang, ZHANG Li-qin. Antifungal and antibacterial activity of extracts from the husk of Carya cathayensis[J]. Journal of Zhejiang A&F University, 2008, 25(3): 355-358.

山核桃外果皮提取物抑菌活性的初步研究

1.
浙江林学院林业与生物技术学院, 浙江临安 311300

基金项目:

浙江省重大科学技术攻关项目(2004C22029)

详细信息

作者简介: 苏秀,助理实验师,硕士,从事生物防治研究。E-mail:susan2046@126.com

中图分类号: S789.5;Q946.8

Antifungal and antibacterial activity of extracts from the husk of Carya cathayensis

1.
School of Forestry and Biotechnology, Zhejiang Forestry College, Lin'an 311300, Zhejiang, China

摘要: 采用甲醇溶剂对山核桃Carya cathayensis外果皮进行提取,并用提取物对番茄灰霉病菌Botrytis cinerea,番茄早疫病菌Alternaria solani,黄瓜枯萎病菌Fusarium oxysporum f.cucumerinum,黄瓜炭疽病菌Colletotrichum lagenarium,棉花枯萎病菌Fusarium oxysporum f.vasinfectum等15种病菌进行抑菌试验。结果表明:在供试质量浓度为干样100 g·L-1时,山核桃外果皮甲醇提取物对黄瓜炭疽病菌、黄瓜菌核病菌、苹果腐烂病菌和辣椒疫病菌等4种病菌的抑制率为100%,除玉米小斑病菌Bipolaris maydis外,对其余的10种病菌抑制率都在69%以上。对其中6种供试病菌菌丝生长的毒力测定试验表明:山核桃外果皮甲醇提取物对水稻纹枯病菌Rhizoctonia solan的菌丝生长抑制作用最好(半数效应质量浓度为29.8 g·L-1),其次为番茄灰霉病菌(半数效应质量浓度为31.1 g·L-1),对玉米大斑病菌Exserohilum turcicum作用最差(半数效应质量浓度为46.2 g·L-1)。利用山核桃外果皮开发植物源杀菌剂值得进一步研究。
- 森林保护学 /
- 山核桃 /
- 外果皮提取物 /
- 抑菌作用
Abstract: The last research showed the husk of Carya cathayensis contained compounds which can control fungi and bacteries. The antifungal and antibacterial activity of methanol extract from the husk of Carya cathayensis was tested with Botrytis cinerea, Alternaria solani, Fusarium oxysporum f.cucumerinum, Colletotrichum lagenarium, Fusarium oxysporum f.vasinfectum, Pyricularia oryzae, Exserohilum turcicum, Bipolaris maydis, Valsa mali, Fusarium graminerum, Rhizoctinia solani, Sclerotinia sclerotiorum, Sclerotinia sclerotiorum, Venturia nashicola and Phytophora capsici by growth speed rate method. Results showed that the extract from the husk of Carya cathayensis in a concentration of 100 g·L-1 had 100% inhibition rates on hyphae growth of Colletotrichum lagenarium, Sclerotinia sclerotiorum, Valsa mali, Phytophthora capsici, whereas, except Bipolaris maydis, there was greater than 69% inhibition rates on the other 10 fungi. The virulence test for six of the 15(Sclerotinia sclerotiorum,Fusarium graminerum,Rhizoctinia solani, Pyricularia oryzae, Exserohilum turcicum, Botrytis cinerea) tested plant pathogens showed that the antifungal activity of the methanol extract from the husk of C. cathayensis was the highest against Rhizoctinia solan with a median effective concentration required to induce a 50% effect (CE50) of 29.8 g·L-1; next was against Botrytis cinerea(CE50 of 31.1 g·L-1), and the lowest was against Exserohilum turcicum(CE50 of 46.2 g·L-1). Thus, research and development of botanical fungicides using the husk of Carya cathayensis should be studied further.
- forest protection /
- Carya cathayensis /
- extracts from husk /
- antifungal activity

[1]	马艳, 史黎央, 赵艺, 徐华潮. 云斑天牛对不同受害状态下山核桃挥发物成分的触角电位和行为反应 . 浙江农林大学学报, 2019, 36(3): 437-443. doi: 10.11833/j.issn.2095-0756.2019.03.002
[2]	石红静, 马闪闪, 赵科理, 叶立前, 李皓, 沈颖, 赵伟明, 叶正钱. 有机物料对酸化山核桃林地土壤的改良作用 . 浙江农林大学学报, 2017, 34(4): 670-678. doi: 10.11833/j.issn.2095-0756.2017.04.013
[3]	张冬勇, 周书永, 朱宁, 樊建庭. 山核桃天牛危害调查及种类的鉴别方法 . 浙江农林大学学报, 2017, 34(1): 42-49. doi: 10.11833/j.issn.2095-0756.2017.01.007
[4]	杜和芬, 王佩星, 徐华潮, 张娓娓, 王紫薇. 光肩星天牛对山核桃挥发性组分的触角电位分析 . 浙江农林大学学报, 2016, 33(1): 166-171. doi: 10.11833/j.issn.2095-0756.2016.01.022
[5]	张爽, 张璞瑜, 蒋达青, 张媚, 张立钦, 林海萍. 棘托竹荪抑菌物质的乙醇提取工艺优化与抑菌作用 . 浙江农林大学学报, 2016, 33(6): 1045-1051. doi: 10.11833/j.issn.2095-0756.2016.06.017
[6]	侯传明, 郑雅文, 王正加, 徐英武. 山核桃MADS-like基因的克隆与分析 . 浙江农林大学学报, 2015, 32(1): 33-39. doi: 10.11833/j.issn.2095-0756.2015.01.005
[7]	王璇, 马良进, 吕全, 孟娴静, 张星耀. 山核桃干腐病病原菌的鉴定 . 浙江农林大学学报, 2014, 31(2): 238-245. doi: 10.11833/j.issn.2095-0756.2014.02.012
[8]	叶申怡, 阮夏云, 樊莹, 张欢帅, 井长怡, 毛胜凤, 张爱莲. 美国山核桃叶的抗菌作用 . 浙江农林大学学报, 2014, 31(4): 658-662. doi: 10.11833/j.issn.2095-0756.2014.04.025
[9]	朱杭瑞, 庄晓伟, 潘炘, 陈顺伟, 章江丽. 山核桃等3类果蓬原料机制炭燃烧性能分析 . 浙江农林大学学报, 2013, 30(1): 90-94. doi: 10.11833/j.issn.2095-0756.2013.01.013
[10]	张璐璐, 贾桂民, 叶建丰, 马良进. 浙江临安山核桃干腐病发生发展规律 . 浙江农林大学学报, 2013, 30(1): 148-152. doi: 10.11833/j.issn.2095-0756.2013.01.022
[11]	巨云为, 樊培峰, 奚月明, 薛中官. 万寿菊提取物对松材线虫的毒杀作用 . 浙江农林大学学报, 2010, 27(2): 316-319. doi: 10.11833/j.issn.2095-0756.2010.02.026
[12]	林君阳, 马良进, 陈安良, 张立钦. 山核桃外果皮化学成分及抑菌活性初步研究 . 浙江农林大学学报, 2009, 26(1): 100-104.
[13]	马良进, 吴美卿, 苏秀, 陈安良, 张立钦. 山核桃外果皮提取物活体抑菌活性 . 浙江农林大学学报, 2009, 26(5): 620-624.
[14]	张昕, 张立钦, 马良进, 林海萍, 毛胜凤, 张炳欣. 生防菌ZJY-1抑菌作用的初步研究 . 浙江农林大学学报, 2007, 24(1): 91-95.
[15]	胡国良, 程益鹏, 楼君芳, 王国杜, 夏国华, 陈建刚, 章祖平. 山核桃花蕾蛆生物学特性及防治技术 . 浙江农林大学学报, 2007, 24(4): 463-467.
[16]	殷舒, 毛胜凤, 杨琼霞, 黄佩龙, 朱栋奎, 胡军祥. 山核桃叶片提取物的抑菌作用 . 浙江农林大学学报, 2007, 24(5): 604-607.
[17]	黄有军, 王正加, 郑炳松, 黄坚钦. 山核桃根插试验 . 浙江农林大学学报, 2006, 23(2): 228-231.
[18]	黄有军, 王正加, 郑炳松, 黄晓敏, 黄坚钦. 山核桃雄蕊发育的解剖学研究 . 浙江农林大学学报, 2006, 23(1): 56-60.
[19]	杨琼霞, 殷舒, 申屠垠, 高欣, 孙益, 胡军祥. 山核桃外果皮提取液对小鼠的辐射防护作用 . 浙江农林大学学报, 2006, 23(6): 604-607.
[20]	黄坚钦, 章滨森, 陆建伟, 付敢伟. 山核桃嫁接愈合过程的解剖学观察 . 浙江农林大学学报, 2001, 18(2): 111-114.

链接本文:
https://zlxb.zafu.edu.cn/article/id/2853

https://zlxb.zafu.edu.cn/article/zjnldxxb/2008/3/355

点击查看大图

计量

文章访问数: 1011
HTML全文浏览量: 166
PDF下载量: 110
被引次数: 0

山核桃外果皮提取物抑菌活性的初步研究

1. 浙江林学院林业与生物技术学院, 浙江临安 311300

基金项目:

浙江省重大科学技术攻关项目(2004C22029)

作者简介:
苏秀,助理实验师,硕士,从事生物防治研究。E-mail:susan2046@126.com

收稿日期: 2007-06-30
修回日期: 2007-12-28
刊出日期: 2008-06-10

中图分类号: S789.5;Q946.8

关键词:

摘要: 采用甲醇溶剂对山核桃Carya cathayensis外果皮进行提取,并用提取物对番茄灰霉病菌Botrytis cinerea,番茄早疫病菌Alternaria solani,黄瓜枯萎病菌Fusarium oxysporum f.cucumerinum,黄瓜炭疽病菌Colletotrichum lagenarium,棉花枯萎病菌Fusarium oxysporum f.vasinfectum等15种病菌进行抑菌试验。结果表明:在供试质量浓度为干样100 g·L-1时,山核桃外果皮甲醇提取物对黄瓜炭疽病菌、黄瓜菌核病菌、苹果腐烂病菌和辣椒疫病菌等4种病菌的抑制率为100%,除玉米小斑病菌Bipolaris maydis外,对其余的10种病菌抑制率都在69%以上。对其中6种供试病菌菌丝生长的毒力测定试验表明:山核桃外果皮甲醇提取物对水稻纹枯病菌Rhizoctonia solan的菌丝生长抑制作用最好(半数效应质量浓度为29.8 g·L-1),其次为番茄灰霉病菌(半数效应质量浓度为31.1 g·L-1),对玉米大斑病菌Exserohilum turcicum作用最差(半数效应质量浓度为46.2 g·L-1)。利用山核桃外果皮开发植物源杀菌剂值得进一步研究。

English Abstract

Antifungal and antibacterial activity of extracts from the husk of Carya cathayensis

1. School of Forestry and Biotechnology, Zhejiang Forestry College, Lin'an 311300, Zhejiang, China

Received Date: 2007-06-30
Rev Recd Date: 2007-12-28
Publish Date: 2008-06-10

Keywords:

Abstract: The last research showed the husk of Carya cathayensis contained compounds which can control fungi and bacteries. The antifungal and antibacterial activity of methanol extract from the husk of Carya cathayensis was tested with Botrytis cinerea, Alternaria solani, Fusarium oxysporum f.cucumerinum, Colletotrichum lagenarium, Fusarium oxysporum f.vasinfectum, Pyricularia oryzae, Exserohilum turcicum, Bipolaris maydis, Valsa mali, Fusarium graminerum, Rhizoctinia solani, Sclerotinia sclerotiorum, Sclerotinia sclerotiorum, Venturia nashicola and Phytophora capsici by growth speed rate method. Results showed that the extract from the husk of Carya cathayensis in a concentration of 100 g·L-1 had 100% inhibition rates on hyphae growth of Colletotrichum lagenarium, Sclerotinia sclerotiorum, Valsa mali, Phytophthora capsici, whereas, except Bipolaris maydis, there was greater than 69% inhibition rates on the other 10 fungi. The virulence test for six of the 15(Sclerotinia sclerotiorum,Fusarium graminerum,Rhizoctinia solani, Pyricularia oryzae, Exserohilum turcicum, Botrytis cinerea) tested plant pathogens showed that the antifungal activity of the methanol extract from the husk of C. cathayensis was the highest against Rhizoctinia solan with a median effective concentration required to induce a 50% effect (CE50) of 29.8 g·L-1; next was against Botrytis cinerea(CE50 of 31.1 g·L-1), and the lowest was against Exserohilum turcicum(CE50 of 46.2 g·L-1). Thus, research and development of botanical fungicides using the husk of Carya cathayensis should be studied further.

苏秀, 马良进, 陈安良, 张立钦. 山核桃外果皮提取物抑菌活性的初步研究[J]. 浙江农林大学学报, 2008, 25(3): 355-358.

引用本文:

苏秀, 马良进, 陈安良, 张立钦. 山核桃外果皮提取物抑菌活性的初步研究[J]. 浙江农林大学学报, 2008, 25(3): 355-358.

SU Xiu, MA Liang-jin, CHEN An-liang, ZHANG Li-qin. Antifungal and antibacterial activity of extracts from the husk of Carya cathayensis[J]. Journal of Zhejiang A&F University, 2008, 25(3): 355-358.

Citation: