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摘要: 利用聚丙烯酰胺垂直板电泳技术对3个睡莲Nymphaea品种彼得Peter Slocum,玛珊姑娘Masaniello和得克萨斯Texas Dawn不同器官(未展开的幼叶、展开的幼叶、老叶、花瓣、花蕾、叶柄)和未展开的幼叶在营养生长阶段和生殖生长阶段的过氧化物酶(POD)和酯酶(EST)同工酶进行了研究。结果表明:①3个品种共有12种过氧化物酶酶带,6种酯酶酶带。②睡莲的过氧化物酶和酯酶具有组织特异性,其中未展开嫩叶和展开嫩叶的酶带最多,叶柄和花蕾次之,花瓣和老叶最少。未展开幼叶为较适合用于睡莲同工酶分析。③与生殖生长阶段相比,3个睡莲品种未展开幼叶在营养生长阶段的酯酶表达量更大,条带更多;而过氧化物酶同工酶表达量则是生殖生长阶段的表达量大,但3个品种的酶条带变化不一致,其中彼得和玛珊姑娘在生殖生长阶段的过氧化物酶同工酶阶段条带多得克萨斯则相反。图4表3参15
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关键词:
- 植物学 /
- 睡莲 /
- 组织特异性 /
- 过氧化物酶(POD) /
- 酯酶(EST)
Abstract: Peroxidase isozyme(POD) and esterase isozyme(EST) of three water lily cultivars,Nymphaea Peter Slocum,N. Masaniello,and N. Texas Dawn,were studied using polyacrylamide gel electrophoresis(PAGE) in different organs (closed young leaf,open young leaf,old leaf,petal,flower bud and petiole) and growth stages of a closed young leaf. Results showed (1) 12 POD bands and 6 EST bands. Also,(2) POD and EST were tissue specific for the closed young leaf,open young leaf,old leaf,petal,bud,and petiole. The most isozyme bands were observed in the closed young leaf and the open young leaf with the least in the old leaf and petal. (3) In addition,compared to the reproductive growth stage,the closed leaf in the vegetative growth stage had more EST and EST bands. Meanwhile,there was more POD during the reproductive stage. However,during the reproductive growth stage more POD bands of N.Peter Slocum and N. Masaniello were found with more POD bands of N.Texas Dawn noted during the vegetative growth stage. [Ch,4 fig. 3 tab. 15 ref.]-
Key words:
- botany /
- water lily /
- tissue specificity /
- peroxidase isozyme (POD) /
- esterase isozyme (EST)
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2010.02.014
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