Mechanical damage on secondary metabolites from Artemisia frigida leaves
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摘要: 为了探讨放牧过程中的采食和践踏损伤对草原植物冷蒿Artemisia frigida次生代谢产物的影响,对盆栽的内蒙古草原冷蒿进行不同程度的机械损伤处理(以剪刀损伤和揉捏),设置轻度(处理1/4枝条)、中度(处理1/3枝条)、重度损伤(处理1/2枝条)水平和对照,利用气相色谱?鄄质谱(GC?鄄MS)联用技术分析了不同处理后冷蒿叶片次生代谢产物的变化。结果表明:机械损伤处理后,冷蒿叶片次生代谢产物的总量在一定的时间范围内增加,轻度处理后次生代谢产物含量的增加较缓慢且峰值出现晚,而中度和重度处理后次生代谢产物的含量迅速增加,在6 h时达到峰值而后出现下降的现象;轻度和中度处理次生代谢产物种类增加,重度处理则减少。冷蒿的次生代谢产物主要是萜烯类化合物,占总量的80%以上;各处理萜烯类化合物含量与对照比分别增加了59%,54%,67%,其中,8-羟基里哪醇的增加趋势最为明显;酚类、醇类、芳香烃和酮类化合物的含量在处理后也有一定程度的增加。图5表1参23
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关键词:
- 植物化学 /
- 机械损伤 /
- 冷蒿 /
- 次生代谢产物 /
- 气相色谱-质谱联用技术
Abstract: To determine the effects of feed intake and trample damage on secondary metabolites of Artemisia frigida, A. frigida from Inner Mongolia grassland was potted in the laboratory and treated with different degrees of mechanical damage (light, moderate, severe, and a control group without damage). Then chemical components of the leaves were analyzed by gas chromatography/mass spectrometry (GC-MS). Results showed that with mechanical damage treatment the total amount of secondary metabolites increased within a certain time range. Secondary metabolites with light damage increased gradually and peaked at 24 h; moderate and severe treatments increased rapidly, peaked at 6 h, and then declined. Increases in species of secondary metabolites were visible when treated with light and moderate damage, but a decrease appeared with severe damage. The main components of secondary metabolites were terpenoids which accounted for more than 80% of the total. The terpenoid content when compared with the control increased 59% with light damage, 54% with moderate damage, and 67% with heavy damage. The increase of 8-hydroxylinalool was greatest; however, phenols, alcohols, aromatics, and ketones also increased. [Ch, 5 fig. 1 tab. 23 ref.] -
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2015.06.004

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