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摘要: 以花叶花秆绿竹Bambusa oldhamii f. variegata腋芽为外植体,筛选增殖最佳芽数3个丛-1来研究不同种类和质量浓度的植物生长调节剂对花叶花秆绿竹增殖的影响。结果表明:单独添加时,6?鄄苄基腺嘌呤(BA)和噻二唑苯基脲(TDZ)效果优于激动素(KT)和2?鄄异戊烯基腺嘌呤(2ip);BA和TDZ相互作用时,效果优于各自单独添加。当0.01 mgL-1 TDZ与3.00 mgL-1BA结合时,芽体增殖最佳,增殖系数达4.63,芽丛生长旺盛。外植体经50.00 mgL -1吲哚丁酸(IBA)处理1 d,再转移到MS(Murashige and Skoog)基本培养基上,发根率最大,达55%,根系发达,试管苗移栽成活率达85%以上。图1表4参13Abstract: Bambusa oldhamii f. variegata is a form of Bambusa oldhamii,having good ornamental and economic value. This aim is to establish a tissue culture system for its commercial propagation. Multiplication of B. oldhamii f. variegata using different plant growth regulators and concentrations was studied with the lateral buds as the explant and three shoots per cluster. The results showed that 0.1 mgL-1 thidiazuron(TDZ) was best with the proliferation rate up to 3.60 when TDZ(0.000 1,0.001 0,0.010 0,0.100 0, 1.000 0 mgL-1),6-benzylaminopurine(BA)(1,3,5,10 mgL-1)、2-isopentenyladenine (2ip) (1,3, 5,10 mgL-1) and kinetin (KT)(1,3,5,10 mgL-1) were added respectively to determine suitable concentration for tissue culture,but the highest proliferation rate 4.63 was obtained with 3.00 mgL-1 BA + 0.01 mgL-1 TDZ when the combination of BA(0.001,0.010,0.100,1.000,3.000 mgL-1),TDZ(0.000 1, 0.001 0,0.010 0,0.100 0 mgL-1),and also good growing. Three shoots per cluster was treated only with 50.00 mgL-1 indole-3-butyric acid(IBA) for 1 day,then the explants were transferred to MS(Murashige and Skoog) basic medium,After 30 days the rooting rate was up to 55 percent,and developed well. The survival rate of transplanted potted plants was above 85 percent.[Ch,1 fig. 4 tab. 13 ref.]
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Key words:
- silviculture /
- Bambusa oldhamii f. variegata /
- tissue culture /
- clustered shoot /
- rapid propagation
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2010.01.024
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