Pollen germination in Camellia oleifera with ascorbic acid and plant growth regulators
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摘要: 以油茶华硕Camellia oleiferaHuashuo花粉为试材,运用琼脂培养基萌发法研究维生素C(AsA)及植物生长调节物质对油茶花粉萌发率的影响。结果表明:适宜质量浓度的维生素(CAsA),吲哚乙酸(IAA),萘乙酸(NAA),2,4-二氯苯氧乙酸(2,4-D)和赤霉素(GA3)对油茶花粉萌发率具有显著促进作用,其中单因素处理AsA质量浓度为20 mgL-1时,花粉萌发率达到最高(65.86%),比对照提高了23.80%;GA3质量浓度为10 mgL-1时花粉萌发率最高,达74.22%;NAA和2,4-D处理花粉萌发率在质量浓度为1.0 mgL-1时最高,分别达到61.33%和60.09%,IAA在质量浓度为5.0 mgL-1时花粉萌发率达到最大(71.52%);适宜质量浓度的IAA,GA3和AsA配合使用能显著提高油茶花粉萌发率。根据适宜质量浓度设计3因素4水平正交实验,发现以5.0 mgL-1 IAA + 5.0 mgL-1GA3 + 20.0 mgL-1AsA最优,油茶花粉萌发率达到82.91%。图3表3参13Abstract: The effects of ascorbic acid(AsA) and plant growth regulators,such as gibberellic acid (GA3),naphthalene acetic acid (NAA),2,4-dichlorophenoxyacetic acid(2,4-D),and indoleacetic acid (IAA) on pollen germination percentage of Camellia oleifera Huashuo were studied with in vitro tissue culture. Results for AsA alone showed that an optimal concentration of 20 mgL-1 increased(P<0.05) the pollen germination rate to 65.9%,which was 23.8% higher than the control(no AsA). The optimal concentration for GA3 alone was 10.0 mgL-1 with a pollen germination rate of 74.22%(P<0.05). The best germination rate was found with 1.0 mgL-1 NAA,1.0 mgL-1 2,4-D),and 5.0 mgL-1 IAA respectively. However,the best results (pollen germination of 82.91%) was with a combination of 5.0 mgL-1 IAA + 5.0 mgL-1 GA3 + 20.0 mgL-1 AsA.[Ch,3 fig. 3 tab. 13 ref.]
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Key words:
- cash forestry /
- Camellia oleifera /
- plant growth regulator /
- pollen /
- germination percentage
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2010.06.023
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