Effects of ultrasonic wave and osmotic treatments on germination of Pinus tabulaeformis seed
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摘要: 通过正交试验设计(L934),研究了超声波和渗透调节处理对油松Pinus tabulaeformis种子萌发和萌发前期物质代谢的影响。结果表明:聚乙二醇(PEG)渗透调节和超声波处理会对油松种子萌发和萌发前期物质代谢产生一定的影响,但具体影响效果不同。PEG促进种子萌发的作用随着溶液质量分数的增大而增强,其中质量分数为20%PEG溶液对油松种子萌发具有显著促进作用,并显著促进种子萌发前期可溶性糖和可溶性蛋白质质量分数的增加。59 kHz超声波处理油松种子10 min,可显著提高种子发芽率和发芽指数,同时加速种子萌发前期淀粉的分解,并提高可溶性蛋白的质量分数。超声波和PEG渗透调节处理油松种子最佳组合为质量分数为20%PEG溶液浸种24 h,然后经59 kHz超声波处理10 min。表3参17Abstract: The effects of ultrasonic wave and polyethylene glycol(PEG) osmotic treatments on germination and the metabolic mechanism during initial germinating stages of Pinus tabulaeformis seeds were studied through an orthogonal design(L934). The results showed that:PEG osmotic adjustment and ultrasonic treatments have different effects on the seed germination and the metabolic mechanism during initial germinating stages of Pinus tabulaeformis seeds. The effect of PEG promotes seed germination,which increases with the increment of PEG soluble concentration,and 20% PEG solution significantly promotes seed germination and significantly increases the content of soluble sugar and soluble protein during the initial germinating stages. The germination rate and germination index could be significantly increased,the decomposition of starch could be accelerated,and the content of soluble protein could be increased during initial germinating stages after the seeds treated with 59 kHz ultrasonic wave on 10 min. The best treatment combination of ultrasonic and PEG osmotic adjustment treatment was:the seeds soaked in 20% PEG solution for 24 h,and then treated with 59 kHz ultrasonic wave for 10 min. [Ch,3 tab. 17 ref.]
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Key words:
- silviculture /
- ultrasonic wave /
- osmotic adjustment /
- inus tabulaeformis /
- seed germination
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2011.04.004

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