Biological and virulence study of highly pathogenic Beauveria bassiana on pine sawyer Monochamus alternatus
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摘要: 对松墨天牛Monochamus alternatus优良球孢白僵菌Beauveria bassiana菌株Bxs的营养生长、产孢条件以及对松墨天牛毒力进行研究。试验表明:Bxs菌株营养生长温度范围为20~30 ℃,最适宜营养生长和产孢温度均为25 ℃,35 ℃下营养生长停止;适合菌株Bxs的营养生长和产孢的培养基为DPSA。毒力测定表明:不同松墨天牛虫龄和虫态间感病性差异较大(P<0.05),虫龄越小越容易感病。Bxs菌株对3龄幼虫的剂量死亡率方程为y = -1.388 4+0.996 7x,致死中浓度CL50为3.936 106个孢子L-1。充分考虑了时间剂量死亡率之间的三维关系制作出了菌株对松墨天牛3龄幼虫的互补重对数(CLL)模型。图3表7参11Abstract: The colony growth and sporogenous conditions of the highly pathogenic strain Bxs of Beauveria bassiana on pine sawyer Monochamus alternatus,and the virulence of Bxs to pine sawyer Monochamus alternatus were studied. The results showed that the growth temperature for Bxs ranged from 20 to 30 ℃,and 25 ℃ was optimal for both colony growth and sporulation,but the colony growth stopped at 35 ℃. For growth and sporulation,DPSA was the desirable culture medium. Virulence tests indicated that great variations existed in disease susceptibility among different developmental stages of Monochamus alternatus,and higher disease sus-ceptibility occurred in the juvenile stages. The equation of dose-mortality and LC50 of the third instar were y=-1.388 4+0.996 7x and 3 936 sporesmL-1 respectively. Based on the Three-dimensional Relation of time-dosage-mortality,a complementary log-log model (CLL model) of Bxs virulence to third instar M. alternatus was constructed.[Ch,3 fig. 7 tab. 11 ref.]
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Key words:
- forest protection /
- Monochamus alternatus /
- Beauveria bassiana /
- biology /
- virulence
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2013.01.012
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