Volume 27 Issue 2
Apr.  2010
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ZHANG Hai-ping, FANG Wei-min, CHEN Fa-di, DING Yue-sheng, YANG Xue-meng. Isozyme analysis for different organs and growth stages of water lily[J]. Journal of Zhejiang A&F University, 2010, 27(2): 246-250. doi: 10.11833/j.issn.2095-0756.2010.02.014
Citation: ZHANG Hai-ping, FANG Wei-min, CHEN Fa-di, DING Yue-sheng, YANG Xue-meng. Isozyme analysis for different organs and growth stages of water lily[J]. Journal of Zhejiang A&F University, 2010, 27(2): 246-250. doi: 10.11833/j.issn.2095-0756.2010.02.014

Isozyme analysis for different organs and growth stages of water lily

doi: 10.11833/j.issn.2095-0756.2010.02.014
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  • Corresponding author: FANG Wei-min
  • Publish Date: 2010-04-20
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Isozyme analysis for different organs and growth stages of water lily

doi: 10.11833/j.issn.2095-0756.2010.02.014
    Corresponding author: FANG Wei-min

Abstract: Peroxidase isozyme(POD) and esterase isozyme(EST) of three water lily cultivars,Nymphaea Peter Slocum,N. Masaniello,and N. Texas Dawn,were studied using polyacrylamide gel electrophoresis(PAGE) in different organs (closed young leaf,open young leaf,old leaf,petal,flower bud and petiole) and growth stages of a closed young leaf. Results showed (1) 12 POD bands and 6 EST bands. Also,(2) POD and EST were tissue specific for the closed young leaf,open young leaf,old leaf,petal,bud,and petiole. The most isozyme bands were observed in the closed young leaf and the open young leaf with the least in the old leaf and petal. (3) In addition,compared to the reproductive growth stage,the closed leaf in the vegetative growth stage had more EST and EST bands. Meanwhile,there was more POD during the reproductive stage. However,during the reproductive growth stage more POD bands of N.Peter Slocum and N. Masaniello were found with more POD bands of N.Texas Dawn noted during the vegetative growth stage. [Ch,4 fig. 3 tab. 15 ref.]

ZHANG Hai-ping, FANG Wei-min, CHEN Fa-di, DING Yue-sheng, YANG Xue-meng. Isozyme analysis for different organs and growth stages of water lily[J]. Journal of Zhejiang A&F University, 2010, 27(2): 246-250. doi: 10.11833/j.issn.2095-0756.2010.02.014
Citation: ZHANG Hai-ping, FANG Wei-min, CHEN Fa-di, DING Yue-sheng, YANG Xue-meng. Isozyme analysis for different organs and growth stages of water lily[J]. Journal of Zhejiang A&F University, 2010, 27(2): 246-250. doi: 10.11833/j.issn.2095-0756.2010.02.014

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