Volume 29 Issue 5
Sep.  2012
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GUAN Yu, JIA Wen-qing, LIU Hui-chao, ZHANG Zhi-jun. Pollen viability and storage of Chaenomeles sinensis[J]. Journal of Zhejiang A&F University, 2012, 29(5): 790-794. doi: 10.11833/j.issn.2095-0756.2012.05.024
Citation: GUAN Yu, JIA Wen-qing, LIU Hui-chao, ZHANG Zhi-jun. Pollen viability and storage of Chaenomeles sinensis[J]. Journal of Zhejiang A&F University, 2012, 29(5): 790-794. doi: 10.11833/j.issn.2095-0756.2012.05.024

Pollen viability and storage of Chaenomeles sinensis

doi: 10.11833/j.issn.2095-0756.2012.05.024
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  • Corresponding author: ZHANG Zhi-jun
  • Received Date: 2011-09-15
  • Rev Recd Date: 2011-11-26
  • Publish Date: 2012-10-20
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Pollen viability and storage of Chaenomeles sinensis

doi: 10.11833/j.issn.2095-0756.2012.05.024
    Corresponding author: ZHANG Zhi-jun

Abstract: To determine the pollen viability of Chaenomeles sinensis,methods of culture medium,iodine/potassium iodide solution (I-KI) staining and 1% 2,3,5-triphenyl tetrazolium chloride (TTC) staining were used to determine the pollen germination. Effects on pollen germination for different storage types and time were also studied. Results showed that the best culture medium was 100 mgL-1 sucrose + 30 mgL-1 boric acid + 8 gL-1 agar,with the germination rate reaching 75.1%. For pollen viability,TTC staining with a germination rate of 60.6% was suitable; however,I-KI staining with germination rate of only 17.9% was not suitable. The best storage temperatures,in turn,were -80 ℃,4 ℃,-23 ℃ and room temperature. At -80 ℃,the germination rate of pollen was 22.9% when it was preserved for 16 days.[Ch,2 fig. 2 tab. 10 ref.]

GUAN Yu, JIA Wen-qing, LIU Hui-chao, ZHANG Zhi-jun. Pollen viability and storage of Chaenomeles sinensis[J]. Journal of Zhejiang A&F University, 2012, 29(5): 790-794. doi: 10.11833/j.issn.2095-0756.2012.05.024
Citation: GUAN Yu, JIA Wen-qing, LIU Hui-chao, ZHANG Zhi-jun. Pollen viability and storage of Chaenomeles sinensis[J]. Journal of Zhejiang A&F University, 2012, 29(5): 790-794. doi: 10.11833/j.issn.2095-0756.2012.05.024

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