Tissue culture and rapid propagation of Ficus pandurata var.angustifolia with different plant growth regulators

Key words: 
• botany /
• Ficus pandurata var. angustifolia /
• callus induction /
• differentiation /
• rooting

Abstract: Little information on callus induction, proliferation and regeneration from leaf explants of Ficus pandurata var. angustifolia, a commonly used medication with the She ethnic minority, has been found. To develop and utilize this ethnic herb, an in vitro induction and shoot regeneration from leaf explants was evaluated using an orthogonal experimental design with plant growth regulators，including 6-Benzylaminopurine（6-BA）, 2,4-dichlorophenoxyacetic acid（2,4-D）, Thidiazuron（TDZ）, and naphthaleneacetic acid（NAA）， concentrations in a Murashige and Skoog（MS） media. Results showed that callus induction ratio in all 9 treatments exceeded 100%, but adventitious buds were extremely difficult to differentiate. The optimum medium for adventitious buds differentiation was MS + 1.0 mgL－1 6-BA + 0.2 mgL－1 2,4-D + 0.1 mgL－1 NAA, the differentiation rate of adventitious buds reached 6.7%. The orthogonal experimental design showed that the proliferation capacity of plant growth regulators was in the order: 6-BA ＞ TDZ ＞NAA. The optimum medium for proliferation was MS + 0.3 mgL－1 TDZ + 1.0 mgL－1 6-BA + 0.3 mgL－1 NAA, the multiplication rate was 8.93, and the rooting rate in all 9 treatments was 100%. The tissue culture technique and rapid propagation system of Ficus pandurata var. angustifolia could be used for large-scale seedlings in short time and provide technical guidance for large-scale production.［Ch, 1 tab. 4 tab. 13 ref.］