Oligomeric status of the SOC1 gene from Phyllostachys violascens and Arabidopsis thaliana

SHI Quan; CHEN Xiaopei; LIN Xinchun; XU Yonghan; XU Yingwu

doi:10.11833/j.issn.2095-0756.2016.02.001

Volume 33 Issue 2

Mar. 2016

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SHI Quan, CHEN Xiaopei, LIN Xinchun, XU Yonghan, XU Yingwu. Oligomeric status of the SOC1 gene from Phyllostachys violascens and Arabidopsis thaliana[J]. Journal of Zhejiang A&F University, 2016, 33(2): 183-190. doi: 10.11833/j.issn.2095-0756.2016.02.001

Citation:

SHI Quan, CHEN Xiaopei, LIN Xinchun, XU Yonghan, XU Yingwu. Oligomeric status of the SOC1 gene from Phyllostachys violascens and Arabidopsis thaliana[J]. Journal of Zhejiang A&F University, 2016, 33(2): 183-190. doi: 10.11833/j.issn.2095-0756.2016.02.001

Oligomeric status of the SOC1 gene from Phyllostachys violascens and Arabidopsis thaliana

doi: 10.11833/j.issn.2095-0756.2016.02.001

1.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Funds:

国家自然科学基金资助项目（31270715，31000295）;浙江省林学一级重中之重学科开放基金资助项目（KF201304）;国家重点基础研究发展计划（973计划）项目（2012CB723008）;浙江农林大学研究生创新基金资助项目（3122013240226）

Received Date: 2015-04-03
Rev Recd Date: 2015-05-12
Publish Date: 2016-04-20

Abstract

MADS-box genes are a super family, which can form different polymers to mediate the development of floral primordia and floral organs. The important one of MADs-box genes, SOC1 ［suppressor of overexpression of cytochrome oxidase 1］, determine the flowering time. The MADS-box transcription factor, SOC1 contains four different functions domains: MADS, I, K, and C. The K domain controls the homologous or heterologous protein polymer formation. The I domain could stabilize the MADS-box proteins binding the DNA. In order to control the floral development, MADS-box proteins can form different complex. Visible difference in floral timing is demonstrated in Phyllostachys violascens and Arabidopsis thaliana. A. thaliana is certain in floral timing while Phyllostachys violascens is not. Though we cannot compare the difference in floral timing with the difference in SOC1 complex formation, it is still interesting. MADS-box genes such as SOC1 containing four different functions: MADS, I, K and C, control floral timing. To determine whether SOC1s from different species demonstrated similar patterns in forming a complex, this study analyzed the differences of polymer formation for SOC1 that originated from Phyllostachys violascens and Arabidopsis thaliana using a yeast two-hybrid assay. Results of the assay showed that the full-length AtSOC1 gene from A. thaliana formed strong homo-polymers with the I and K domains being the main regions for formation of the interaction. However, the full-length SOC1 from Phyllostachys violascens （PvSOC1） did not form a homodimer. Thus, even though the K protein domain could form a homodimer, the presence of the I protein domain disrupted the dimer meaning the I domain could be a key factor in SOC1 polymer formation; the role of the I domain in floral timing should be verified with a plant transgene study.［Ch, 5 fig. 2 tab. 29 ref.］
- botany,
- MADS-box,
- SOC1,
- Phyllostachys violascens,
- Arabidopsis thaliana,
- yeast two-hybrid,
- polymer,
- I and K protein domain

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Oligomeric status of the SOC1 gene from Phyllostachys violascens and Arabidopsis thaliana

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Funds:

Received Date: 2015-04-03

Rev Recd Date: 2015-05-12

Publish Date: 2016-04-20

Key words:

Abstract: MADS-box genes are a super family, which can form different polymers to mediate the development of floral primordia and floral organs. The important one of MADs-box genes, SOC1 ［suppressor of overexpression of cytochrome oxidase 1］, determine the flowering time. The MADS-box transcription factor, SOC1 contains four different functions domains: MADS, I, K, and C. The K domain controls the homologous or heterologous protein polymer formation. The I domain could stabilize the MADS-box proteins binding the DNA. In order to control the floral development, MADS-box proteins can form different complex. Visible difference in floral timing is demonstrated in Phyllostachys violascens and Arabidopsis thaliana. A. thaliana is certain in floral timing while Phyllostachys violascens is not. Though we cannot compare the difference in floral timing with the difference in SOC1 complex formation, it is still interesting. MADS-box genes such as SOC1 containing four different functions: MADS, I, K and C, control floral timing. To determine whether SOC1s from different species demonstrated similar patterns in forming a complex, this study analyzed the differences of polymer formation for SOC1 that originated from Phyllostachys violascens and Arabidopsis thaliana using a yeast two-hybrid assay. Results of the assay showed that the full-length AtSOC1 gene from A. thaliana formed strong homo-polymers with the I and K domains being the main regions for formation of the interaction. However, the full-length SOC1 from Phyllostachys violascens （PvSOC1） did not form a homodimer. Thus, even though the K protein domain could form a homodimer, the presence of the I protein domain disrupted the dimer meaning the I domain could be a key factor in SOC1 polymer formation; the role of the I domain in floral timing should be verified with a plant transgene study.［Ch, 5 fig. 2 tab. 29 ref.］

Oligomeric status of the SOC1 gene from Phyllostachys violascens and Arabidopsis thaliana

doi: 10.11833/j.issn.2095-0756.2016.02.001

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

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