Comparison of methods of extracting genomic DNA from <em>Betula luminifera</em>

XIE Yi-qing; LI Zhi-zhen; HUANG Ru-zhu; XIAO Xiang-xi; WANG Zhi-jie

Volume 23 Issue 6

Feb. 2017

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Article Navigation > Journal of Zhejiang A&F University > 2006 > 23(6): 664-668

XIE Yi-qing, LI Zhi-zhen, HUANG Ru-zhu, XIAO Xiang-xi, WANG Zhi-jie. Comparison of methods of extracting genomic DNA from Betula luminifera[J]. Journal of Zhejiang A&F University, 2006, 23(6): 664-668.

Citation:

XIE Yi-qing, LI Zhi-zhen, HUANG Ru-zhu, XIAO Xiang-xi, WANG Zhi-jie. Comparison of methods of extracting genomic DNA from Betula luminifera[J]. Journal of Zhejiang A&F University, 2006, 23(6): 664-668.

Comparison of methods of extracting genomic DNA from Betula luminifera

1.
Fujian Academy of Forestry , Fuzhou 350012 , Fujian, China;
2.
College of Life Sciences, Fujian Normal University , Fuzhou 350007, Fujian, China

Publish Date: 2006-12-20

Abstract

Six methods including basic CTAB method , modified CTAB methods as CTAB I , CTAB Ⅱ , CTAB Ⅲ , CTAB Ⅳ , and improved SDS methods were used to extract high-quality DNA from the young leaves of Betula luminifera .The DNA samples obtained by the above methods were tested by agarose-gel electrophoresis , restricted enzyme digests and PCR. Results showed that the modified methods were better than the basic CTAB method in terms of the quality of total DNA , but the effects of DNA extraction differed greatly . Among them, the modified CTAB I method was the best for extracting B .luminifera DNA from leaves .The comparisons of PCR patterns of genomic DNA extracted with different methods showed that different extracting methods would affect the changes of PCR patterns .[ Ch , 3 fig .1 tab .13 ref .]
- botany,
- Betula luminifera,
- DNA extracting,
- CTAB method,
- SDS method,
- recalcitrant plant taxa

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Comparison of methods of extracting genomic DNA from Betula luminifera

1. Fujian Academy of Forestry , Fuzhou 350012 , Fujian, China;

2. College of Life Sciences, Fujian Normal University , Fuzhou 350007, Fujian, China

Publish Date: 2006-12-20

Key words:

Abstract: Six methods including basic CTAB method , modified CTAB methods as CTAB I , CTAB Ⅱ , CTAB Ⅲ , CTAB Ⅳ , and improved SDS methods were used to extract high-quality DNA from the young leaves of Betula luminifera .The DNA samples obtained by the above methods were tested by agarose-gel electrophoresis , restricted enzyme digests and PCR. Results showed that the modified methods were better than the basic CTAB method in terms of the quality of total DNA , but the effects of DNA extraction differed greatly . Among them, the modified CTAB I method was the best for extracting B .luminifera DNA from leaves .The comparisons of PCR patterns of genomic DNA extracted with different methods showed that different extracting methods would affect the changes of PCR patterns .[ Ch , 3 fig .1 tab .13 ref .]

Comparison of methods of extracting genomic DNA from Betula luminifera

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Comparison of methods of extracting genomic DNA from Betula luminifera

1. Fujian Academy of Forestry , Fuzhou 350012 , Fujian, China;

2. College of Life Sciences, Fujian Normal University , Fuzhou 350007, Fujian, China

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Comparison of methods of extracting genomic DNA from Betula luminifera

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Comparison of methods of extracting genomic DNA from Betula luminifera

1. Fujian Academy of Forestry , Fuzhou 350012 , Fujian, China; 2. College of Life Sciences, Fujian Normal University , Fuzhou 350007, Fujian, China

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1. Fujian Academy of Forestry , Fuzhou 350012 , Fujian, China;

2. College of Life Sciences, Fujian Normal University , Fuzhou 350007, Fujian, China