In vitro rapid propagation of <em>Hedera nepalensis var .sinensis</em>

ZHANG Cun-xu; YANG Feng-li; YUAN Xiu-ping

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Volume 22 Issue 2

Feb. 2017

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Article Navigation > Journal of Zhejiang A&F University > 2005 > 22(2): 241-245

ZHANG Cun-xu, YANG Feng-li, YUAN Xiu-ping. In vitro rapid propagation of Hedera nepalensis var .sinensis[J]. Journal of Zhejiang A&F University, 2005, 22(2): 241-245.

Citation:

ZHANG Cun-xu, YANG Feng-li, YUAN Xiu-ping. In vitro rapid propagation of Hedera nepalensis var .sinensis[J]. Journal of Zhejiang A&F University, 2005, 22(2): 241-245.

In vitro rapid propagation of Hedera nepalensis var .sinensis

1.
.Institute of Forestry Science , Northwest A&F University , Yangling 712100 , Shaanxi , China;
2.
Department of Forestry , Yangling Vocational and Technical College , Yangling 712100, Shaanxi , China

Funds:

陕西省科技攻关项目(2001K01-G7-01)

Received Date: 2004-07-06
Rev Recd Date: 2005-01-18
Publish Date: 2005-06-20

Abstract

Using nodal segments as a source of initial explants , the effects of basal medium, plant growth substances as well as transplantation medium on rapid propagation of Hedera neplensis var .sinensis was studied . The results showed that the rich salt medium MS is more suitable for initial and multiplication culture than low salt media WPM and B5 .It is better to add 2.0mgL-16-BA and 1.0 mgL-1GA3 to multiplicationmedium , in which multiplication coefficient reached 4 .Although high rooting percentage was obtained with free growth regulators on 1/2 MS medium , root system development could be improved on medium by adding 0.10 mgL-1 NAA .The survival rate of transplanation was about 80 % in humus soil .Using tap water and edible white sugar instead of distilled water and sucrose have no significant effect on shoots multiplication and growth of H .nepalensis var . sinensis , so the culture cost could be reduced .[ Ch , 6 tab .9 ref .]
- nursery stock growing,
- Hedera nepalensis var .sinensis,
- rapid propagation,
- in vitro culture

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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In vitro rapid propagation of Hedera nepalensis var .sinensis

1. .Institute of Forestry Science , Northwest A&F University , Yangling 712100 , Shaanxi , China;

2. Department of Forestry , Yangling Vocational and Technical College , Yangling 712100, Shaanxi , China

Funds:

陕西省科技攻关项目(2001K01-G7-01)

Received Date: 2004-07-06
Rev Recd Date: 2005-01-18
Publish Date: 2005-06-20

Key words:

Abstract: Using nodal segments as a source of initial explants , the effects of basal medium, plant growth substances as well as transplantation medium on rapid propagation of Hedera neplensis var .sinensis was studied . The results showed that the rich salt medium MS is more suitable for initial and multiplication culture than low salt media WPM and B5 .It is better to add 2.0mgL-16-BA and 1.0 mgL-1GA3 to multiplicationmedium , in which multiplication coefficient reached 4 .Although high rooting percentage was obtained with free growth regulators on 1/2 MS medium , root system development could be improved on medium by adding 0.10 mgL-1 NAA .The survival rate of transplanation was about 80 % in humus soil .Using tap water and edible white sugar instead of distilled water and sucrose have no significant effect on shoots multiplication and growth of H .nepalensis var . sinensis , so the culture cost could be reduced .[ Ch , 6 tab .9 ref .]

ZHANG Cun-xu, YANG Feng-li, YUAN Xiu-ping. In vitro rapid propagation of Hedera nepalensis var .sinensis[J]. Journal of Zhejiang A&F University, 2005, 22(2): 241-245.

Citation:

ZHANG Cun-xu, YANG Feng-li, YUAN Xiu-ping. In vitro rapid propagation of Hedera nepalensis var .sinensis[J]. Journal of Zhejiang A&F University, 2005, 22(2): 241-245.

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In vitro rapid propagation of Hedera nepalensis var .sinensis

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In vitro rapid propagation of Hedera nepalensis var .sinensis

1. .Institute of Forestry Science , Northwest A&F University , Yangling 712100 , Shaanxi , China;

2. Department of Forestry , Yangling Vocational and Technical College , Yangling 712100, Shaanxi , China

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In vitro rapid propagation of Hedera nepalensis var .sinensis

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通讯作者: 陈斌, bchen63@163.com

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In vitro rapid propagation of Hedera nepalensis var .sinensis

1. .Institute of Forestry Science , Northwest A&F University , Yangling 712100 , Shaanxi , China; 2. Department of Forestry , Yangling Vocational and Technical College , Yangling 712100, Shaanxi , China

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1. .Institute of Forestry Science , Northwest A&F University , Yangling 712100 , Shaanxi , China;

2. Department of Forestry , Yangling Vocational and Technical College , Yangling 712100, Shaanxi , China