Genetic analysis of RAPDs in <em>Chamaecyparis obtusa</em> using partial diallel test

TANG Ding-qin; INOUE Makoto; KONDO Akira; IDE Yuji

Volume 21 Issue 3

Feb. 2017

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2004 > 21(3): 235-242

TANG Ding-qin, INOUE Makoto, KONDO Akira, IDE Yuji. Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test[J]. Journal of Zhejiang A&F University, 2004, 21(3): 235-242.

Citation:

TANG Ding-qin, INOUE Makoto, KONDO Akira, IDE Yuji. Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test[J]. Journal of Zhejiang A&F University, 2004, 21(3): 235-242.

Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test

1.
.Key Lab forModern Silvicultural Technology of Zhejiang Province, Zhejiang Forestry College, Lin’an 311300 , Zhejiang , China;
2.
.University Forest in Aichi , The University Forests , Faculty of Agriculture , The University of Tokyo , Tokyo 1 1 3 , Japan;
3.
.Shizuoka Prefecture Forest ry and Forest Products Research Institute , Hamakita 4 3 7 , Negata , Japan;
4.
.Graduate S chool of Agricultural and Life Sciences , The University of Tokyo, Tokyo 113 , Japan

Funds:

This research was supported by a grant-in-aid No .07556035 of the Ministry of Education Science and Culture of Japan .

More Information

Author Bio:
TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn

TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn
Received Date: 2004-03-16
Rev Recd Date: 2004-05-19
Publish Date: 2004-09-20

Abstract

To develop molecular markers for Chamaecyparis obtusa , the inheritance of RAPD fragments was studied in diploid tissues of 5 parents and 30 diploid controlled F1 progenies in each partial diallel crosses of these parents .Of 46 primers tested , 18 primers yielded 135 reproducible fragments , of which 42 fragments (31 %)were polymorphic among the parents .Fourteen fragments amplified from 7 primers were selected to test the segregation among controlled F1 progenies .All fragments observed in the parents were found in the progeny .Segregation of all variable fragments observed in diploid materials fitted the proportions expected for a dominant Mendelian trait .The segregating fragments were either present or absent confirming the dominant character of RAPD variation .The usefulness of RAPD fragments as genetic markers for estimating genetic diversity was also discussed .[ En , 1 fig .2 tab .18 ref .]
- Chamaecyparis obtusa,
- diallel crosses,
- RAPD,
- segregation analysis

Relative Article

[1]	LUO Jiayan, WANG Ying, ZHONG Wenhan, LIU Haichong, JIANG Huojun. Establishment of RAPD and ISSR markers for wood identification of 16 species of Lauraceae . Journal of Zhejiang A&F University, 2017, 34(5): 942-948. doi: 10.11833/j.issn.2095-0756.2017.05.023
[2]	SONG Minguo, YUAN Jinqiang, YANG Xianyu, ZHANG Shufang, ZHUGE Hui, XU Yue. Cloning and sequence analysis of prothymosin-α cDNA of Bufo japonicus formosus . Journal of Zhejiang A&F University, 2013, 30(3): 401-405. doi: 10.11833/j.issn.2095-0756.2013.03.016
[3]	RUAN Jiajia, YUAN Jinqiang, YANG Xianyu. Cloning and sequence analysis of claudin-4 cDNA from Bufo japonicus formosus . Journal of Zhejiang A&F University, 2013, 30(4): 561-566. doi: 10.11833/j.issn.2095-0756.2013.04.016
[4]	ZHANG Zhen, ZHANG Han-guo, ZHANG Lei, ZHU Hang-yong, LI Xue-feng. Diversity analysis with RAPD for natural and breeding populations of Larix gmelinii . Journal of Zhejiang A&F University, 2012, 29(1): 130-136. doi: 10.11833/j.issn.2095-0756.2012.01.022
[5]	LI Yuan-chun, SHEN Lin, ZENG Yan-ru. Establishment of a SRAP analysis protocol in Carya cathayensis and a comparison among SRAP，RAPD，ISSR analysis protocols . Journal of Zhejiang A&F University, 2011, 28(3): 505-512. doi: 10.11833/j.issn.2095-0756.2011.03.025
[6]	GAO Yan-hui, FAN Min-liang, LUO Wen-jian, HUANG Hua-hong, TONG Zai-kang. RAPD analysis of genetic diversity for Pseudolarix amabilis： a critically endangered plant . Journal of Zhejiang A&F University, 2011, 28(5): 815-822. doi: 10.11833/j.issn.2095-0756.2011.05.023
[7]	LAN Yun-long, WU Ling-shang, QIU Bo-yin, GAO Yan-hui, SI Jin-ping. Houttuynia cordata analysis with RAPD markers . Journal of Zhejiang A&F University, 2008, 25(3): 309-313.
[8]	CAO Fu-liang, HUA Zhe-bin, WANG Gui-bin, ZHANG Wang-xiang. Genetic diversity in wild populations of Ginkgo biloba using random amplified polymorphic DNA (RAPD) analysis . Journal of Zhejiang A&F University, 2008, 25(1): 22-27.
[9]	WANG Zu-liang, DING Li-xia, ZHAO Ming-shui, CHENG Xiao-yuan, SHEN Qian. Genetic diversity of Ostrya rehderiana revealed by RAPD markers . Journal of Zhejiang A&F University, 2008, 25(3): 304-308.
[10]	YANG Jing-yuan, XIONG Dan, LIANG Hong-wei, WANG Yu-bing, LIAOMing-ya, CHEN Fa-ju. Optimal reaction system for random amplified polymorphic DNA with endangered Emmenopterys henryi . Journal of Zhejiang A&F University, 2007, 24(3): 279-283.
[11]	YANG Yi, ZHOU Zu-ji, ZHANG Li-qin, MA Liang-jin, LIN Xin-chun, GAO Jie. RAPD analysis of DNA polymorphism of parasitic Beauveria bassiana in Monochamus alternatus . Journal of Zhejiang A&F University, 2005, 22(3): 306-309.
[12]	ZENG Yan-ru, HU Ying, SI Jin-ping, XU Xiang-hua. RAPD analysis of 16 orange varieties in Lishui of Zhejiang . Journal of Zhejiang A&F University, 2005, 22(5): 481-485.
[13]	CHENG Xiao-jian, YANG Ping, LIN Bo-nian, ZHENG Bing-song. Genetic relationship of myrobalan plum, plum and apricot cultivars by RAPD analysis . Journal of Zhejiang A&F University, 2004, 21(1): 40-43.
[14]	WANG Zheng-jia, HUANG Jian-qin, GUO Chuan-you, YANG Ping, WANG Hua-fang. The optimal reaction system of RAPD in Carya cathayensis . Journal of Zhejiang A&F University, 2003, 20(4): 429-433.
[15]	JIN Ze-xin, LI Jun-min, ZHONG Zhang-cheng. Optimization of RAPD conditions of Sargentodoxa cuneata . Journal of Zhejiang A&F University, 2003, 20(2): 141-145.
[16]	LEI Ri-ping, CHEN Hui, XIE Li-guo. Analysis of the genetic distance of Castanea henryi with RAPD markers . Journal of Zhejiang A&F University, 2002, 19(3): 240-243.
[17]	FANG Wei, HE Zheng-xiang, HUANG Jian-qin, SHI Qian-jun, LIN Xin-chun. Studies on cultivars of Phyllostachys pxaecox with RAPD molecular markers . Journal of Zhejiang A&F University, 2001, 18(1): 1-5.
[18]	ZHANG Li-qin, LIN Xin-chun, MAO Sheng-feng. Analysis on isolates of Metarrhizium anisopliae by RAPD . Journal of Zhejiang A&F University, 2001, 18(3): 281-285.
[19]	LIANG Yue-rong, TANAKA Juni-chi, TAKEDA Yoshi-yuki. Study on diversity of tea germplasm by RAPD method . Journal of Zhejiang A&F University, 2000, 17(2): 215-218.
[20]	Zhang Jianzhang You Chang shun Lǜ Jinsong, . Combining ability analysis on growth traits of half-diallel cross progeny of Chinese fir . Journal of Zhejiang A&F University, 1998, 15(2): 138-144.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(1022) PDF downloads(193) Cited by()

Proportional views

Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test

1. .Key Lab forModern Silvicultural Technology of Zhejiang Province, Zhejiang Forestry College, Lin’an 311300 , Zhejiang , China;

2. .University Forest in Aichi , The University Forests , Faculty of Agriculture , The University of Tokyo , Tokyo 1 1 3 , Japan;

3. .Shizuoka Prefecture Forest ry and Forest Products Research Institute , Hamakita 4 3 7 , Negata , Japan;

4. .Graduate S chool of Agricultural and Life Sciences , The University of Tokyo, Tokyo 113 , Japan

Funds:

This research was supported by a grant-in-aid No .07556035 of the Ministry of Education Science and Culture of Japan .

Author Bio:

Received Date: 2004-03-16

Rev Recd Date: 2004-05-19

Publish Date: 2004-09-20

Key words:

Abstract: To develop molecular markers for Chamaecyparis obtusa , the inheritance of RAPD fragments was studied in diploid tissues of 5 parents and 30 diploid controlled F1 progenies in each partial diallel crosses of these parents .Of 46 primers tested , 18 primers yielded 135 reproducible fragments , of which 42 fragments (31 %)were polymorphic among the parents .Fourteen fragments amplified from 7 primers were selected to test the segregation among controlled F1 progenies .All fragments observed in the parents were found in the progeny .Segregation of all variable fragments observed in diploid materials fitted the proportions expected for a dominant Mendelian trait .The segregating fragments were either present or absent confirming the dominant character of RAPD variation .The usefulness of RAPD fragments as genetic markers for estimating genetic diversity was also discussed .[ En , 1 fig .2 tab .18 ref .]

Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test

HTML

Catalog

Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test

HTML

Catalog

Export File

Citation

Format

Content