Genetic analysis of RAPDs in <em>Chamaecyparis obtusa</em> using partial diallel test

TANG Ding-qin; INOUE Makoto; KONDO Akira; IDE Yuji

Volume 21 Issue 3

Feb. 2017

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TANG Ding-qin, INOUE Makoto, KONDO Akira, IDE Yuji. Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test[J]. Journal of Zhejiang A&F University, 2004, 21(3): 235-242.

Citation:

TANG Ding-qin, INOUE Makoto, KONDO Akira, IDE Yuji. Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test[J]. Journal of Zhejiang A&F University, 2004, 21(3): 235-242.

Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test

1.
.Key Lab forModern Silvicultural Technology of Zhejiang Province, Zhejiang Forestry College, Lin’an 311300 , Zhejiang , China;
2.
.University Forest in Aichi , The University Forests , Faculty of Agriculture , The University of Tokyo , Tokyo 1 1 3 , Japan;
3.
.Shizuoka Prefecture Forest ry and Forest Products Research Institute , Hamakita 4 3 7 , Negata , Japan;
4.
.Graduate S chool of Agricultural and Life Sciences , The University of Tokyo, Tokyo 113 , Japan

Funds:

This research was supported by a grant-in-aid No .07556035 of the Ministry of Education Science and Culture of Japan .

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Author Bio:
TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn

TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn
Received Date: 2004-03-16
Rev Recd Date: 2004-05-19
Publish Date: 2004-09-20

Abstract

To develop molecular markers for Chamaecyparis obtusa , the inheritance of RAPD fragments was studied in diploid tissues of 5 parents and 30 diploid controlled F1 progenies in each partial diallel crosses of these parents .Of 46 primers tested , 18 primers yielded 135 reproducible fragments , of which 42 fragments (31 %)were polymorphic among the parents .Fourteen fragments amplified from 7 primers were selected to test the segregation among controlled F1 progenies .All fragments observed in the parents were found in the progeny .Segregation of all variable fragments observed in diploid materials fitted the proportions expected for a dominant Mendelian trait .The segregating fragments were either present or absent confirming the dominant character of RAPD variation .The usefulness of RAPD fragments as genetic markers for estimating genetic diversity was also discussed .[ En , 1 fig .2 tab .18 ref .]
- Chamaecyparis obtusa,
- diallel crosses,
- RAPD,
- segregation analysis

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沈阳化工大学材料科学与工程学院沈阳 110142

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Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test

1. .Key Lab forModern Silvicultural Technology of Zhejiang Province, Zhejiang Forestry College, Lin’an 311300 , Zhejiang , China;

2. .University Forest in Aichi , The University Forests , Faculty of Agriculture , The University of Tokyo , Tokyo 1 1 3 , Japan;

3. .Shizuoka Prefecture Forest ry and Forest Products Research Institute , Hamakita 4 3 7 , Negata , Japan;

4. .Graduate S chool of Agricultural and Life Sciences , The University of Tokyo, Tokyo 113 , Japan

Funds:

This research was supported by a grant-in-aid No .07556035 of the Ministry of Education Science and Culture of Japan .

Author Bio:

Received Date: 2004-03-16

Rev Recd Date: 2004-05-19

Publish Date: 2004-09-20

Key words:

Abstract: To develop molecular markers for Chamaecyparis obtusa , the inheritance of RAPD fragments was studied in diploid tissues of 5 parents and 30 diploid controlled F1 progenies in each partial diallel crosses of these parents .Of 46 primers tested , 18 primers yielded 135 reproducible fragments , of which 42 fragments (31 %)were polymorphic among the parents .Fourteen fragments amplified from 7 primers were selected to test the segregation among controlled F1 progenies .All fragments observed in the parents were found in the progeny .Segregation of all variable fragments observed in diploid materials fitted the proportions expected for a dominant Mendelian trait .The segregating fragments were either present or absent confirming the dominant character of RAPD variation .The usefulness of RAPD fragments as genetic markers for estimating genetic diversity was also discussed .[ En , 1 fig .2 tab .18 ref .]

Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test

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