野生银杏资源群体遗传多样性的RAPD分析

曹福亮; 花喆斌; 汪贵斌; 张往祥

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野生银杏资源群体遗传多样性的RAPD分析

曹福亮 , 花喆斌 , 汪贵斌 , 张往祥

文章导航 > 浙江农林大学学报 > 2008 > 25(1): 22-27

曹福亮, 花喆斌, 汪贵斌, 张往祥. 野生银杏资源群体遗传多样性的RAPD分析[J]. 浙江农林大学学报, 2008, 25(1): 22-27.

引用本文:

曹福亮, 花喆斌, 汪贵斌, 张往祥. 野生银杏资源群体遗传多样性的RAPD分析[J]. 浙江农林大学学报, 2008, 25(1): 22-27.

CAO Fu-liang, HUA Zhe-bin, WANG Gui-bin, ZHANG Wang-xiang. Genetic diversity in wild populations of Ginkgo biloba using random amplified polymorphic DNA (RAPD) analysis[J]. Journal of Zhejiang A&F University, 2008, 25(1): 22-27.

Citation:

CAO Fu-liang, HUA Zhe-bin, WANG Gui-bin, ZHANG Wang-xiang. Genetic diversity in wild populations of Ginkgo biloba using random amplified polymorphic DNA (RAPD) analysis[J]. Journal of Zhejiang A&F University, 2008, 25(1): 22-27.

野生银杏资源群体遗传多样性的RAPD分析

1.
南京林业大学森林资源与环境学院, 江苏南京 210037

基金项目:

江苏省高技术研究项目(BG2004314)

详细信息

作者简介: 曹福亮,教授,博士生导师,从事经济林培育等的研究。E-mail:samcao2007@yahoo.com.cn

中图分类号: S718.46;Q813

Genetic diversity in wild populations of Ginkgo biloba using random amplified polymorphic DNA (RAPD) analysis

1.
College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu, China

摘要: 用随机扩增多态DNA (RAPD)技术对浙江西天目山、贵州务川、湖北大洪山区、重庆金佛山区和福建武夷山区等5个群体150个银杏Ginkgo biloba个体进行了遗传多样性分析。12个寡核苷酸引物共检测到194个位点,多态位点130个,多态位点百分率66.67%。用PopGen 32软件对数据进行了分析,结果显示:Nei's基因多样性指数(h)的群体水平是0.431 7,Shannon信息指数(I)的群体水平是0.621 1,基因分化系数(Gst)是0.288 2。Shannon信息指数(I)分析揭示的银杏群体遗传分化水平=0.190 3,AMOVA分析结果得出银杏群体间的分化占36.7%。RAPD数据显示,5个银杏群体遗传多样性较高,其中浙江西天目山、贵州务川和湖北大洪山区有可能是野生银杏冰川期避难所。
- 林木遗传育种学 /
- 银杏 /
- 遗传多样性 /
- 随机扩增多态DNA(RAPD)
Abstract: To determine the genetic variation among and within the populations of Ginkgo biloba, 150 individuals from 5 populations that were located in the Tianmu Mountain area(Zhejiang Province), Wuchuan(Guizhou Province),the Dahong Mountain area(Hubei Province), the Jingfu Mountain area(Chongqing City),and the Wuyi Mountain area(Fujian Province) were analyzed using random amplified polymorphic DNA(RAPD) markers with 12 random primers. Data were analyzed with the PopGen32 software and analysis of molecular variance(AMOVA), and compared using the Nei, Shannon, among-population genetic diversity(Gst) indices. RAPD detected 194 loci of which 130 exhibited polymorphism with an average percentage of polymorphic loci(PPB) of 66.7%. Also,the average for Nei's genetic diversity was 0.431 7, for Shannon's genetic diversity information index was 0.621 1, and Gst was 0.288 2. In addition,Shannon's genetic diversity information index revealed a differentiation among populations of 0.190 3, whereas the AMOVA showing differentiation among populations was 36.7%. Overall, RAPD results indicated that the five populations of G. biloba had high genetic diversity with the populations of Tianmu Mountain area, Wuchuan, and Dahong Mountain area possibly being survival habitats stemming from the glacial period.
- forest breeding /
- Ginkgo biloba /
- genetic diversity /
- random amplified polymorphic DNA(RAPD)

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野生银杏资源群体遗传多样性的RAPD分析

1. 南京林业大学森林资源与环境学院, 江苏南京 210037

基金项目:

江苏省高技术研究项目(BG2004314)

作者简介:
曹福亮,教授,博士生导师,从事经济林培育等的研究。E-mail:samcao2007@yahoo.com.cn

收稿日期: 2007-01-23
修回日期: 2007-09-11
刊出日期: 2008-02-10

中图分类号: S718.46;Q813

关键词:

摘要: 用随机扩增多态DNA (RAPD)技术对浙江西天目山、贵州务川、湖北大洪山区、重庆金佛山区和福建武夷山区等5个群体150个银杏Ginkgo biloba个体进行了遗传多样性分析。12个寡核苷酸引物共检测到194个位点,多态位点130个,多态位点百分率66.67%。用PopGen 32软件对数据进行了分析,结果显示:Nei's基因多样性指数(h)的群体水平是0.431 7,Shannon信息指数(I)的群体水平是0.621 1,基因分化系数(Gst)是0.288 2。Shannon信息指数(I)分析揭示的银杏群体遗传分化水平=0.190 3,AMOVA分析结果得出银杏群体间的分化占36.7%。RAPD数据显示,5个银杏群体遗传多样性较高,其中浙江西天目山、贵州务川和湖北大洪山区有可能是野生银杏冰川期避难所。

English Abstract

Genetic diversity in wild populations of Ginkgo biloba using random amplified polymorphic DNA (RAPD) analysis

1. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu, China

Received Date: 2007-01-23
Rev Recd Date: 2007-09-11
Publish Date: 2008-02-10

Keywords:

Abstract: To determine the genetic variation among and within the populations of Ginkgo biloba, 150 individuals from 5 populations that were located in the Tianmu Mountain area(Zhejiang Province), Wuchuan(Guizhou Province),the Dahong Mountain area(Hubei Province), the Jingfu Mountain area(Chongqing City),and the Wuyi Mountain area(Fujian Province) were analyzed using random amplified polymorphic DNA(RAPD) markers with 12 random primers. Data were analyzed with the PopGen32 software and analysis of molecular variance(AMOVA), and compared using the Nei, Shannon, among-population genetic diversity(Gst) indices. RAPD detected 194 loci of which 130 exhibited polymorphism with an average percentage of polymorphic loci(PPB) of 66.7%. Also,the average for Nei's genetic diversity was 0.431 7, for Shannon's genetic diversity information index was 0.621 1, and Gst was 0.288 2. In addition,Shannon's genetic diversity information index revealed a differentiation among populations of 0.190 3, whereas the AMOVA showing differentiation among populations was 36.7%. Overall, RAPD results indicated that the five populations of G. biloba had high genetic diversity with the populations of Tianmu Mountain area, Wuchuan, and Dahong Mountain area possibly being survival habitats stemming from the glacial period.

曹福亮, 花喆斌, 汪贵斌, 张往祥. 野生银杏资源群体遗传多样性的RAPD分析[J]. 浙江农林大学学报, 2008, 25(1): 22-27.

引用本文:

曹福亮, 花喆斌, 汪贵斌, 张往祥. 野生银杏资源群体遗传多样性的RAPD分析[J]. 浙江农林大学学报, 2008, 25(1): 22-27.

Citation:

CAO Fu-liang, HUA Zhe-bin, WANG Gui-bin, ZHANG Wang-xiang. Genetic diversity in wild populations of Ginkgo biloba using random amplified polymorphic DNA (RAPD) analysis[J]. Journal of Zhejiang A&F University, 2008, 25(1): 22-27.