<em>Rd</em>29A gene cloning and plant expression vector constructed

ZHUO Ren-ying; SUN Zong-xiu

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Volume 21 Issue 3

Feb. 2017

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Article Navigation > Journal of Zhejiang A&F University > 2004 > 21(3): 243-246

ZHUO Ren-ying, SUN Zong-xiu. Rd29A gene cloning and plant expression vector constructed[J]. Journal of Zhejiang A&F University, 2004, 21(3): 243-246.

Citation:

ZHUO Ren-ying, SUN Zong-xiu. Rd29A gene cloning and plant expression vector constructed[J]. Journal of Zhejiang A&F University, 2004, 21(3): 243-246.

Rd29A gene cloning and plant expression vector constructed

ZHUO Ren-ying^{1
,
,},
SUN Zong-xiu²

1.
The Research Institute of Subtropical Forest , CAF, Fuyang 311400 , Zhejiang , China;
2.
The State Key Laboratory of Rice Biology , Chinese National Rice Research Institute , Hangzhou 310006, Zhejiang , China

Funds:

浙江省自然科学基金重点资助项目(ZA0204)

Received Date: 2003-10-20
Rev Recd Date: 2004-05-17
Publish Date: 2004-09-20

Abstract

It was global problem that effected of saline on forest and agriculture soil , about 20 % plowlands and half of irrigated soils had been affected by saline .It's important to speeded salt-tolerance breeding course . Transgenic breeding can fasted the salt-tolerance pace , and shorten breeding cycle significantly .So we designed the primer and cloning Rd 29A promoter by PCR , constucted pBinRdMCS plasmid that BADH gene can be induced by salt and drought , then it was transit to chloroplast .The results analysis by PCR and restriction show that the plasmid include aim gene .The plasmid will be used for tree transgenic breeding .[ Ch , 5 fig .8 ref .]
- forest tree,
- salt tolerance,
- Rd29A promoter,
- BADH

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Rd29A gene cloning and plant expression vector constructed

ZHUO Ren-ying^1, ,,
SUN Zong-xiu²

1. The Research Institute of Subtropical Forest , CAF, Fuyang 311400 , Zhejiang , China;

2. The State Key Laboratory of Rice Biology , Chinese National Rice Research Institute , Hangzhou 310006, Zhejiang , China

Funds:

浙江省自然科学基金重点资助项目(ZA0204)

Received Date: 2003-10-20
Rev Recd Date: 2004-05-17
Publish Date: 2004-09-20

Key words:

Abstract: It was global problem that effected of saline on forest and agriculture soil , about 20 % plowlands and half of irrigated soils had been affected by saline .It's important to speeded salt-tolerance breeding course . Transgenic breeding can fasted the salt-tolerance pace , and shorten breeding cycle significantly .So we designed the primer and cloning Rd 29A promoter by PCR , constucted pBinRdMCS plasmid that BADH gene can be induced by salt and drought , then it was transit to chloroplast .The results analysis by PCR and restriction show that the plasmid include aim gene .The plasmid will be used for tree transgenic breeding .[ Ch , 5 fig .8 ref .]

ZHUO Ren-ying, SUN Zong-xiu. Rd29A gene cloning and plant expression vector constructed[J]. Journal of Zhejiang A&F University, 2004, 21(3): 243-246.

Citation:

ZHUO Ren-ying, SUN Zong-xiu. Rd29A gene cloning and plant expression vector constructed[J]. Journal of Zhejiang A&F University, 2004, 21(3): 243-246.

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Rd29A gene cloning and plant expression vector constructed

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通讯作者: 陈斌, bchen63@163.com

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Rd29A gene cloning and plant expression vector constructed

1. The Research Institute of Subtropical Forest , CAF, Fuyang 311400 , Zhejiang , China;

2. The State Key Laboratory of Rice Biology , Chinese National Rice Research Institute , Hangzhou 310006, Zhejiang , China

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Rd29A gene cloning and plant expression vector constructed

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通讯作者: 陈斌, bchen63@163.com

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Rd29A gene cloning and plant expression vector constructed

1. The Research Institute of Subtropical Forest , CAF, Fuyang 311400 , Zhejiang , China; 2. The State Key Laboratory of Rice Biology , Chinese National Rice Research Institute , Hangzhou 310006, Zhejiang , China

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1. The Research Institute of Subtropical Forest , CAF, Fuyang 311400 , Zhejiang , China;

2. The State Key Laboratory of Rice Biology , Chinese National Rice Research Institute , Hangzhou 310006, Zhejiang , China