Volume 21 Issue 3
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ZHUO Ren-ying, SUN Zong-xiu. Rd29A gene cloning and plant expression vector constructed[J]. Journal of Zhejiang A&F University, 2004, 21(3): 243-246.
Citation: ZHUO Ren-ying, SUN Zong-xiu. Rd29A gene cloning and plant expression vector constructed[J]. Journal of Zhejiang A&F University, 2004, 21(3): 243-246.

Rd29A gene cloning and plant expression vector constructed

Funds:

浙江省自然科学基金重点资助项目(ZA0204)

  • Received Date: 2003-10-20
  • Rev Recd Date: 2004-05-17
  • Publish Date: 2004-09-20
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

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Rd29A gene cloning and plant expression vector constructed

Funds:

浙江省自然科学基金重点资助项目(ZA0204)

Abstract: It was global problem that effected of saline on forest and agriculture soil , about 20 % plowlands and half of irrigated soils had been affected by saline .It's important to speeded salt-tolerance breeding course . Transgenic breeding can fasted the salt-tolerance pace , and shorten breeding cycle significantly .So we designed the primer and cloning Rd 29A promoter by PCR , constucted pBinRdMCS plasmid that BADH gene can be induced by salt and drought , then it was transit to chloroplast .The results analysis by PCR and restriction show that the plasmid include aim gene .The plasmid will be used for tree transgenic breeding .[ Ch , 5 fig .8 ref .]

ZHUO Ren-ying, SUN Zong-xiu. Rd29A gene cloning and plant expression vector constructed[J]. Journal of Zhejiang A&F University, 2004, 21(3): 243-246.
Citation: ZHUO Ren-ying, SUN Zong-xiu. Rd29A gene cloning and plant expression vector constructed[J]. Journal of Zhejiang A&F University, 2004, 21(3): 243-246.

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