Culture condition of phosphate-solubilizing bacteria <em>Bacillus megaterium</em> var .<em>phosphaticum</em>

LIANG Jin-feng; CHEN Xin; TANG Jian-jun

Volume 19 Issue 4

Jul. 2017

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LIANG Jin-feng, CHEN Xin, TANG Jian-jun. Culture condition of phosphate-solubilizing bacteria Bacillus megaterium var .phosphaticum[J]. Journal of Zhejiang A&F University, 2002, 19(4): 342-345.

Citation:

LIANG Jin-feng, CHEN Xin, TANG Jian-jun. Culture condition of phosphate-solubilizing bacteria Bacillus megaterium var .phosphaticum[J]. Journal of Zhejiang A&F University, 2002, 19(4): 342-345.

Culture condition of phosphate-solubilizing bacteria Bacillus megaterium var .phosphaticum

1.
.Faculty of Life Science , Zhejiang Forestry College , Lin'an 311300 , Zhejiang , China;
2.
Faculty of Life Science , Zhejiang University , Hangzhou 310029 , Zhejiang , China

Publish Date: 2002-12-20

Abstract

Influence of different physical and chemical factors on the growth of phosphate-solubilizing bacteria (PSB) were investigated .The results showed that ①PSB was able to thrive and grow very well when its volume in a 100 mL flask was 20 mL , which was considered as optimal .②The bacteria grew well in slightly alkaline conditions and the optimal pH was 8.5 ～ 9.0 .③The temperature for the largest number of cell after two days'incubation was 30 ℃. ④Glucose was the best source of carbon ;the most suitable Nitrogen and Carbon concentrations were 8 gL-1 and 0.21 gL-1 respectively .⑤Mg2 + , Mn2 + , and Ca2 + could improve the quantity of the initial growth of this bacteria .But Fe2 + and Al3 + especially Fe2 + inhibit the bacteria's initial growth .
- phosphate-solubilizing bacteria,
- culture condition,
- phosphorus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Culture condition of phosphate-solubilizing bacteria Bacillus megaterium var .phosphaticum

1. .Faculty of Life Science , Zhejiang Forestry College , Lin'an 311300 , Zhejiang , China;

2. Faculty of Life Science , Zhejiang University , Hangzhou 310029 , Zhejiang , China

Publish Date: 2002-12-20

Key words:

Abstract: Influence of different physical and chemical factors on the growth of phosphate-solubilizing bacteria (PSB) were investigated .The results showed that ①PSB was able to thrive and grow very well when its volume in a 100 mL flask was 20 mL , which was considered as optimal .②The bacteria grew well in slightly alkaline conditions and the optimal pH was 8.5 ～ 9.0 .③The temperature for the largest number of cell after two days'incubation was 30 ℃. ④Glucose was the best source of carbon ;the most suitable Nitrogen and Carbon concentrations were 8 gL-1 and 0.21 gL-1 respectively .⑤Mg2 + , Mn2 + , and Ca2 + could improve the quantity of the initial growth of this bacteria .But Fe2 + and Al3 + especially Fe2 + inhibit the bacteria's initial growth .

Culture condition of phosphate-solubilizing bacteria Bacillus megaterium var .phosphaticum

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通讯作者: 陈斌, bchen63@163.com

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Culture condition of phosphate-solubilizing bacteria Bacillus megaterium var .phosphaticum

1. .Faculty of Life Science , Zhejiang Forestry College , Lin'an 311300 , Zhejiang , China;

2. Faculty of Life Science , Zhejiang University , Hangzhou 310029 , Zhejiang , China

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Culture condition of phosphate-solubilizing bacteria Bacillus megaterium var .phosphaticum

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Culture condition of phosphate-solubilizing bacteria Bacillus megaterium var .phosphaticum

1. .Faculty of Life Science , Zhejiang Forestry College , Lin'an 311300 , Zhejiang , China; 2. Faculty of Life Science , Zhejiang University , Hangzhou 310029 , Zhejiang , China

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1. .Faculty of Life Science , Zhejiang Forestry College , Lin'an 311300 , Zhejiang , China;

2. Faculty of Life Science , Zhejiang University , Hangzhou 310029 , Zhejiang , China