Analysis on isolates of <em>Metarrhizium anisopliae</em> by RAPD

ZHANG Li-qin; LIN Xin-chun; MAO Sheng-feng

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Volume 18 Issue 3

Sep. 2018

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Article Navigation > Journal of Zhejiang A&F University > 2001 > 18(3): 281-285

ZHANG Li-qin, LIN Xin-chun, MAO Sheng-feng. Analysis on isolates of Metarrhizium anisopliae by RAPD[J]. Journal of Zhejiang A&F University, 2001, 18(3): 281-285.

Citation:

ZHANG Li-qin, LIN Xin-chun, MAO Sheng-feng. Analysis on isolates of Metarrhizium anisopliae by RAPD[J]. Journal of Zhejiang A&F University, 2001, 18(3): 281-285.

Analysis on isolates of Metarrhizium anisopliae by RAPD

1.
Department of Resources and Environment, Zhejiang Forestry College, Lin,an, 311300, Zhejiang, China

Received Date: 2001-08-20
Rev Recd Date: 2001-05-20
Publish Date: 2001-09-20

Abstract

DNA of Metarrhizium anisopliae isolates was analyzed by RAPD. Twenty-seven primers screened and optimized out of 160 primers were applied to the proliferation of PCR. Three-hundred-four loci have been proliferated in 4 strains of M .anisopliae, 299 loci out of which are of polymorphism and account for 89.5%. All these suggest that each strain has an abundant genetic polyorphism. There is some correlation between DNA polymorphism of strains and their original hosts, size of conidiospore, distributions, but no obvious correlation between DNA polymorphism and virulence on Monochamus alternatus larvae.
- Metarrhizium anisopliae,
- random amplified polymorphic DNA,
- molecular warkers,
- Monochamus alternatus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Analysis on isolates of Metarrhizium anisopliae by RAPD

1. Department of Resources and Environment, Zhejiang Forestry College, Lin,an, 311300, Zhejiang, China

Received Date: 2001-08-20
Rev Recd Date: 2001-05-20
Publish Date: 2001-09-20

Key words:

Abstract: DNA of Metarrhizium anisopliae isolates was analyzed by RAPD. Twenty-seven primers screened and optimized out of 160 primers were applied to the proliferation of PCR. Three-hundred-four loci have been proliferated in 4 strains of M .anisopliae, 299 loci out of which are of polymorphism and account for 89.5%. All these suggest that each strain has an abundant genetic polyorphism. There is some correlation between DNA polymorphism of strains and their original hosts, size of conidiospore, distributions, but no obvious correlation between DNA polymorphism and virulence on Monochamus alternatus larvae.

ZHANG Li-qin, LIN Xin-chun, MAO Sheng-feng. Analysis on isolates of Metarrhizium anisopliae by RAPD[J]. Journal of Zhejiang A&F University, 2001, 18(3): 281-285.

Citation:

ZHANG Li-qin, LIN Xin-chun, MAO Sheng-feng. Analysis on isolates of Metarrhizium anisopliae by RAPD[J]. Journal of Zhejiang A&F University, 2001, 18(3): 281-285.

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1. Department of Resources and Environment, Zhejiang Forestry College, Lin,an, 311300, Zhejiang, China

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