Expressed salt stress induced full length-cDNA library of <EM>Salix matsudana</EM> using yeast Invsc1

YANG Ye; JIANG Jing; QIAO Gui-rong; ZHOU Jing; CHEN Yin; HE Zheng-quan; LI Hai-ying; ZHUO Ren-ying

Volume 26 Issue 4

Aug. 2009

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2009 > 26(4): 473-478

YANG Ye, JIANG Jing, QIAO Gui-rong, ZHOU Jing, CHEN Yin, HE Zheng-quan, LI Hai-ying, ZHUO Ren-ying. Expressed salt stress induced full length-cDNA library of Salix matsudana using yeast Invsc1[J]. Journal of Zhejiang A&F University, 2009, 26(4): 473-478.

Citation:

YANG Ye, JIANG Jing, QIAO Gui-rong, ZHOU Jing, CHEN Yin, HE Zheng-quan, LI Hai-ying, ZHUO Ren-ying. Expressed salt stress induced full length-cDNA library of Salix matsudana using yeast Invsc1[J]. Journal of Zhejiang A&F University, 2009, 26(4): 473-478.

Expressed salt stress induced full length-cDNA library of Salix matsudana using yeast Invsc1

1.
Biotechnology Research Center / Hubei Province Key Laboratory of Natural Products Research and Development， China Three Gorges University，Yichang 443002，Hubei，China;
2.
Research Institute of Subtropical Forestry，The Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China

More Information

Corresponding author: ZHUO Ren-ying
Publish Date: 2009-08-20

Abstract

The studies of Salix matsudana on salt-resistance molecular mechanism and searching new salt-tolerant relation genes are limited at present. It is necessary to develop drought-tolerant gene using this valuable tree resource，so a salt stress induced full-length cDNA Library of Salix matsudana was constructed using Clontech Laboratories，Inc. SMART cDNA Library Construction Protocol. Total RNA was extracted from S. matsudana under 100 mmol·L-1 NaCl stress for 24 h. After synthesizing the first-strand cDNA，the double-strand cDNA was amplified with the long-distance Polymerase Chain Reaction （LD-PCR）. The result showed that insertion fragment was about 1 000 bp length in the library by PCR. The full-length cDNA fragment was linked to yeast vector pYES2.1 by pYES2.1 TOPO TA Expression Kit；then the linked product was transferred into yeast Invsc1. Finally，clones were selected by SC-U medium containing 80 － 130 g·L－1 NaCl. The experiment shows that the salt-tolerance of transformants yeast improved greatly compared with the wild-type yeast. The results provide an important tool for the study of cloning new salt-tolerance relation genes and the salt tolerance mechanisms of the S. matsudana. ［Ch，6 fig. 17 ref.］
- botany,
- Salix matsudana,
- cDNA library,
- salt tolerance,
- yeast,
- drought tolerance related genes

Relative Article

[1]	HUANG Qingchen, LAI Jianxin, HUANG Lichao, LU Mengzhu. Effects of peroxidase gene PagPRX19 on salt tolerance of poplar ‘84K’ . Journal of Zhejiang A&F University, 2022, 39(6): 1163-1172. doi: 10.11833/j.issn.2095-0756.20220387
[2]	LI Dandan, LIN Rong, LI Xinguo, ZHENG Yueping. Functional analysis of AtJAR1 gene in salt tolerance of Arabidopsis thaliana . Journal of Zhejiang A&F University, 2022, 39(5): 998-1009. doi: 10.11833/j.issn.2095-0756.20210742
[3]	LIU Liping, DAI Fengbin, ZHANG Chong, TIAN Ju, CHEN Jinhuan. Cloning and expression analysis of the SKOR gene for an outward-rectifying K⁺ channel in Lycium ruthenicum . Journal of Zhejiang A&F University, 2018, 35(1): 104-111. doi: 10.11833/j.issn.2095-0756.2018.01.014
[4]	XIN Jianpan, SUN Xinxin, TIAN Runan. Floristic diversity and fundamental characteristics of seed plants on Mount Jiangjun, Nanjing . Journal of Zhejiang A&F University, 2017, 34(4): 629-636. doi: 10.11833/j.issn.2095-0756.2017.04.008
[5]	CHEN Wen, WANG Jing, WU Haiping, ZHANG Ling, YE Zhengqian. Salt tolerance of 5 typical landscape plant species in Zhoushan Islands . Journal of Zhejiang A&F University, 2017, 34(2): 283-293. doi: 10.11833/j.issn.2095-0756.2017.02.012
[6]	ZHAO Ting, HAN Xiaojiao, LIU Mingying, QIAO Guirong, JIANG Jing, JIANG Yancheng, ZHUO Renying. Isolation and expression of a cadmium-resistant gene （SaFer） from Sedum alfredii . Journal of Zhejiang A&F University, 2015, 32(1): 25-32. doi: 10.11833/j.issn.2095-0756.2015.01.004
[7]	ZHANG Mei, LIU Li, Yi lita. Plant floristic diversity in the Baishilazi National Nature Reserve of Liaoning, China . Journal of Zhejiang A&F University, 2014, 31(2): 210-216. doi: 10.11833/j.issn.2095-0756.2014.02.008
[8]	SONG Shuang, HUANG Yin-zhi, DONG Lei-ming, HUANG You-jun, ZENG Yan-ru, WU Zhi-min. Simple sequence repeat （SSR） markers in Carya cathayensis . Journal of Zhejiang A&F University, 2012, 29(4): 626-633. doi: 10.11833/j.issn.2095-0756.2012.04.023
[9]	MA Jin, LIU Zhi-gao, ZHENG Gang. Differential proteomics and its application in plant salt stress . Journal of Zhejiang A&F University, 2011, 28(1): 139-143. doi: 10.11833/j.issn.2095-0756.2011.01.022
[10]	ZHANG Xiao-guo, QIAO Gui-rong, LI Cui-yun, LIU Ming-ying, JIANG Jing, LI Hai-ying, QIU Wen-min, ZHUO Ren-ying. A two-dimensional electrophoresis system of Salix matsudana root proteomics . Journal of Zhejiang A&F University, 2011, 28(4): 653-661. doi: 10.11833/j.issn.2095-0756.2011.04.021
[11]	HUANG Yin-zhi, ZHOU Qin, HUANG You-jun, ZENG Yan-ru. Construction of a fat metabolism-related cDNA library in Carya cathayensis . Journal of Zhejiang A&F University, 2011, 28(1): 80-85. doi: 10.11833/j.issn.2095-0756.2011.01.013
[12]	WANG Hong－ling, ZHANG Jue, JIAO Zhong－yi, SHI De－zong. Establishing and optimizing the SRAP－PCR system for Salix matsudana . Journal of Zhejiang A&F University, 2010, 27(6): 945-949. doi: 10.11833/j.issn.2095-0756.2010.06.024
[13]	SONG Hong－gai, JIANG Jing, QIAO Gui－rong, YANG Ye, ZHOU Jing, PAN Luan－yin, ZHUO Ren－ying. Plant stress－resistant gene cloning for Na and Cd using an INVSc1 yeast . Journal of Zhejiang A&F University, 2010, 27(6): 890-895. doi: 10.11833/j.issn.2095-0756.2010.06.014
[14]	ZHENG Bing-song, CHEN Miao, CHU Huai-liang, AI Xue, HUANG You-jun, LI Xue-qin, HUANG Jian-qin. Auxin response factor fragments cloned by cDNA-AFLP during Carya cathayensis grafting process . Journal of Zhejiang A&F University, 2009, 26(4): 467-472.
[15]	HUANG You-jun, ZHOU Li, CHEN Fang-fang, ZHOU Qin, HUANG Jian-qin, HUANG Min-ren, WANG ming-xiu. Gene expression with cDNA-AFLP （amplified fragment length polymorphism） during flowering of Carya cathayensis . Journal of Zhejiang A&F University, 2009, 26(3): 297-301.
[16]	XU Ai-chun, CHEN Yi-tai, CHEN Qing-hong, WANG Shu-fen. Cd tolerance and leaf physiology changes in ten clones of Salix matsudana with Cd stress . Journal of Zhejiang A&F University, 2009, 26(5): 674-681.
[17]	CHEN Ke, WANG Xiao-de. Physiological indices of three liana species(Mucuna sempervirens, Hedera nepalensis var. sinensis and Euonymus fortunei) with drought resistance . Journal of Zhejiang A&F University, 2008, 25(3): 314-318.
[18]	RUAN Xiu-chun, SI Jin-ping, WU Jian, HUANG Wen-hua, GUO Bao-lin, XU Yuan-ke. Preliminary study on biological characteristics and ecological adaptability of Tripterygium . Journal of Zhejiang A&F University, 2006, 23(5): 595-598.
[19]	Zhang Liqin, Zheng Yong ping, Luo Shiyuan, Hu Jiagong.. Salt Tolerance of Calli of Populus Clones and Pinus elliottii in Tissue Cultures . Journal of Zhejiang A&F University, 1997, 14(1): 16-21.
[20]	Zhang Liqin, Zheng YongPing, Jin Peiying. selection of Salt Tolerance poplar Regenerated plant-lets in Tissues Culture. . Journal of Zhejiang A&F University, 1996, 13(4): 397-404.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(3874) PDF downloads(295) Cited by()

Proportional views

Expressed salt stress induced full length-cDNA library of Salix matsudana using yeast Invsc1

1. Biotechnology Research Center / Hubei Province Key Laboratory of Natural Products Research and Development， China Three Gorges University，Yichang 443002，Hubei，China;

2. Research Institute of Subtropical Forestry，The Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China

Corresponding author: ZHUO Ren-ying

Publish Date: 2009-08-20

Key words:

Abstract: The studies of Salix matsudana on salt-resistance molecular mechanism and searching new salt-tolerant relation genes are limited at present. It is necessary to develop drought-tolerant gene using this valuable tree resource，so a salt stress induced full-length cDNA Library of Salix matsudana was constructed using Clontech Laboratories，Inc. SMART cDNA Library Construction Protocol. Total RNA was extracted from S. matsudana under 100 mmol·L-1 NaCl stress for 24 h. After synthesizing the first-strand cDNA，the double-strand cDNA was amplified with the long-distance Polymerase Chain Reaction （LD-PCR）. The result showed that insertion fragment was about 1 000 bp length in the library by PCR. The full-length cDNA fragment was linked to yeast vector pYES2.1 by pYES2.1 TOPO TA Expression Kit；then the linked product was transferred into yeast Invsc1. Finally，clones were selected by SC-U medium containing 80 － 130 g·L－1 NaCl. The experiment shows that the salt-tolerance of transformants yeast improved greatly compared with the wild-type yeast. The results provide an important tool for the study of cloning new salt-tolerance relation genes and the salt tolerance mechanisms of the S. matsudana. ［Ch，6 fig. 17 ref.］

Expressed salt stress induced full length-cDNA library of Salix matsudana using yeast Invsc1

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Expressed salt stress induced full length-cDNA library of Salix matsudana using yeast Invsc1

1. Biotechnology Research Center / Hubei Province Key Laboratory of Natural Products Research and Development， China Three Gorges University，Yichang 443002，Hubei，China;

2. Research Institute of Subtropical Forestry，The Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China

Corresponding author: ZHUO Ren-ying

HTML

Catalog

Expressed salt stress induced full length-cDNA library of Salix matsudana using yeast Invsc1

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Expressed salt stress induced full length-cDNA library of Salix matsudana using yeast Invsc1

1. Biotechnology Research Center / Hubei Province Key Laboratory of Natural Products Research and Development， China Three Gorges University，Yichang 443002，Hubei，China; 2. Research Institute of Subtropical Forestry，The Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China

Corresponding author: ZHUO Ren-ying

HTML

Catalog

Export File

Citation

Format

Content

1. Biotechnology Research Center / Hubei Province Key Laboratory of Natural Products Research and Development， China Three Gorges University，Yichang 443002，Hubei，China;

2. Research Institute of Subtropical Forestry，The Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China