Optimum conditions for rapid propagation with clones of <i>Acacia implexa</i>

HE Wen-jin; HUANG Qi; CHEN You-qiang; DAI Rong-chun; LIN Si-zu

Dec. 2007

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2007 > 24(6): 775-778

HE Wen-jin, HUANG Qi, CHEN You-qiang, DAI Rong-chun, LIN Si-zu. Optimum conditions for rapid propagation with clones of Acacia implexa[J]. Journal of Zhejiang A&F University, 2007, 24(6): 775-778.

Citation:

HE Wen-jin, HUANG Qi, CHEN You-qiang, DAI Rong-chun, LIN Si-zu. Optimum conditions for rapid propagation with clones of Acacia implexa[J]. Journal of Zhejiang A&F University, 2007, 24(6): 775-778.

Optimum conditions for rapid propagation with clones of Acacia implexa

1. Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China;
2. Bioengineering College, Fujian Normal University, Fuzhou 350007, Fujian, China;
3. Fuzhou Academy of Agriculture Science, Fuzhou 350018, Fujian, China

Received Date: 2006-11-13
Rev Recd Date: 2007-06-11

Abstract

Acacia implexa is an excellent multipurpose tree species in South China, however seed propagation will cause segregation of inheritable characteristics and then, limit its utilization. This study looked at the use of tissue culture to clone trees with three plant growth regulators (6-BA; NAA, ZT) in different levels, and appropriate methods for possible ways to prevent vitrification of adventitious buds by orthogonal experiment L₉(3⁴). Results of experiments on micropropagation of young stem sections for selected adult trees of A. implexa showed that a suitable medium for adventitious bud differentiation of the stem sections with axillary buds was:MS (Murashige and Skoog's medium)+1.0 mg·L^-1 BA(6-benzyladenine) +0.2 mg·L^-1 NAA(α-naphthalene acetic acid)+1.0 mg·L^-1 ZT(zeatin), a suitable medium for adventitious bud multiplication was:1/2 MS(NH⁺₄:NO^-₃=1:2)+1.0 mg·L^-16-BA+0.05 mg·L^-1 NAA, and a suitable medium for improving roots was:1/2 MS+0.1 mg·L^-1 NAA. After transplanting, over 70% of the tube plantlets survived.
- botany,
- Acacia implexa,
- in vitro culture

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Optimum conditions for rapid propagation with clones of Acacia implexa

1. Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China;

2. Bioengineering College, Fujian Normal University, Fuzhou 350007, Fujian, China;

3. Fuzhou Academy of Agriculture Science, Fuzhou 350018, Fujian, China

Received Date: 2006-11-13

Rev Recd Date: 2007-06-11

Key words:

Abstract: Acacia implexa is an excellent multipurpose tree species in South China, however seed propagation will cause segregation of inheritable characteristics and then, limit its utilization. This study looked at the use of tissue culture to clone trees with three plant growth regulators (6-BA; NAA, ZT) in different levels, and appropriate methods for possible ways to prevent vitrification of adventitious buds by orthogonal experiment L₉(3⁴). Results of experiments on micropropagation of young stem sections for selected adult trees of A. implexa showed that a suitable medium for adventitious bud differentiation of the stem sections with axillary buds was:MS (Murashige and Skoog's medium)+1.0 mg·L^-1 BA(6-benzyladenine) +0.2 mg·L^-1 NAA(α-naphthalene acetic acid)+1.0 mg·L^-1 ZT(zeatin), a suitable medium for adventitious bud multiplication was:1/2 MS(NH⁺₄:NO^-₃=1:2)+1.0 mg·L^-16-BA+0.05 mg·L^-1 NAA, and a suitable medium for improving roots was:1/2 MS+0.1 mg·L^-1 NAA. After transplanting, over 70% of the tube plantlets survived.

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Optimum conditions for rapid propagation with clones of Acacia implexa

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通讯作者: 陈斌, bchen63@163.com

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Optimum conditions for rapid propagation with clones of Acacia implexa

1. Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China;

2. Bioengineering College, Fujian Normal University, Fuzhou 350007, Fujian, China;

3. Fuzhou Academy of Agriculture Science, Fuzhou 350018, Fujian, China

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Optimum conditions for rapid propagation with clones of Acacia implexa

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通讯作者: 陈斌, bchen63@163.com

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Optimum conditions for rapid propagation with clones of Acacia implexa

1. Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China; 2. Bioengineering College, Fujian Normal University, Fuzhou 350007, Fujian, China; 3. Fuzhou Academy of Agriculture Science, Fuzhou 350018, Fujian, China

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1. Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China;

2. Bioengineering College, Fujian Normal University, Fuzhou 350007, Fujian, China;

3. Fuzhou Academy of Agriculture Science, Fuzhou 350018, Fujian, China