Journal of Zhejiang A&F University ›› 2015, Vol. 32 ›› Issue (1): 25-32.doi: 10.11833/j.issn.2095-0756.2015.01.004

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Isolation and expression of a cadmium-resistant gene (SaFer) from Sedum alfredii

ZHAO Ting1,2,3, HAN Xiaojiao2,3, LIU Mingying2,3, QIAO Guirong2,3, JIANG Jing2,3,#br# JIANG Yancheng1,ZHUO Renying2,3   

  1. 1. Department of Biology and Technology, Xinjiang University, Urumqi 830046, Xinjiang, China; 2. The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China; 3. State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China
  • Received:2014-03-01 Revised:2014-04-14 Online:2015-02-20 Published:2015-01-15

Abstract:

Abiotic stress was a serious problem that affect plants growth and products. In plants, ferritin is a special iron storage protein closely related to stress. A cDNA from the Sedum alfredii cDNA library, designated as SaFer, was isolated and analyzed by homologous analysis and by Real-Time Reverse Transcription-PCR (qRT-PCR). Then, cadmium-stress experiments were conducted to compare transgenic Arabidopsis thaliana overexpressed SaFer to a wild type. Results showed that SaFer cDNA was 1 117 bp long with an opening reading frame (ORF) of 759 bp. The ORF of SaFer encoded a polypeptide of 252 amino acids with a calculated molecular weight of about 27.8 kDa, and the homologous analysis showed that it was most closely related to ferritin of Malus domestica with 76% identities. The length of the genomic sequence of SaFer was 1 702 bp and contained 7 introns. Expression profiles in roots were analyzed by qRT-PCR and the results showed that the transcription level of SaFer was enhanced after 12 h cadmium stress. Transgenic Arabidopsis thaliana overexpressing SaFer displayed much higher Cd tolerance than the wild type which was further supported by physiological indexes such as SOD activity and electric conductivity. The results showed that SaFer could provide a glimpse into S. alfredii cadmium-tolerance and could contribute to the breeding of cadmium-tolerant plants. [Ch,9 fig. 1 tab. 23 ref. ]

Key words: botany, Sedum alfredii, ferritin, cadmium stress, expression change

CLC Number: 

  • S718.3