Single nucleotide polymorphism of non-ε 14-3-3 genes in Shibataeeae

XU Zaien; YU Yu; LI Bingjuan; CHEN Hongjun; GUO Xiaoqin

doi:10.11833/j.issn.2095-0756.2016.01.011

Volume 33 Issue 1

Jan. 2016

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XU Zaien, YU Yu, LI Bingjuan, CHEN Hongjun, GUO Xiaoqin. Single nucleotide polymorphism of non-ε 14-3-3 genes in Shibataeeae[J]. Journal of Zhejiang A&F University, 2016, 33(1): 80-87. doi: 10.11833/j.issn.2095-0756.2016.01.011

Citation:

XU Zaien, YU Yu, LI Bingjuan, CHEN Hongjun, GUO Xiaoqin. Single nucleotide polymorphism of non-ε 14-3-3 genes in Shibataeeae[J]. Journal of Zhejiang A&F University, 2016, 33(1): 80-87. doi: 10.11833/j.issn.2095-0756.2016.01.011

Single nucleotide polymorphism of non-ε 14-3-3 genes in Shibataeeae

doi: 10.11833/j.issn.2095-0756.2016.01.011

1.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Funds:

国家自然科学基金资助项目（30901155）; 浙江省自然科学基金资助项目（Y307499）

Received Date: 2015-03-04
Rev Recd Date: 2015-04-02
Publish Date: 2016-02-20

Abstract

To study the structure and single nucleotide polymorphism （SNP） of non- 14-3-3 in Shibataeeae, homologue genes from five bamboo species, Hibanobambusa tranquillans, Indosasa shibataeaoides, Phyllostachys edulis, Shibataea chinensis, and Sinobambusa tootsik were cloned with gDNA and cDNA sequences being compared. Results showed five exons and four introns with CDS sequence lengths （in bp） of 789, 771, 789, and 786, respectively. There were 21, 19, 26, and 17 SNP sites in 14-3-3b, 14-3-3c, 14-3-3e, and 14-3-3f coding regions, with SNP frequencies （in bp） of 1SNP/38, 1SNP/41, 1SNP/30, and 1SNP/46, respectively. Nucleotide diversity （） for SNPs was 0.015 21, 0.012 17, 0.010 98, and 0.009 16, in that order. The amino acid sequence identity for these same genes in different species was 99%. Four 14-3-3 putative proteins were hydrophilic, and their isoelectric point was distributed from 4.75 to 4.90 with the amino acids Ala, Ser, and Gly being abundant. Also, -helixs and random coils in these proteins were the most plentiful; whereas, -turns and extended strands were few. These results lay a foundation for deeper studies with biological functions of 14-3-3 in bamboo. ［Ch, 3 fig. 3 tab. 27 ref.］
- molecular genetics,
- Shibataeeae,
- bamboo,
- 14-3-3 gene family,
- single nucleotide polymorphism （SNP）

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Single nucleotide polymorphism of non-ε 14-3-3 genes in Shibataeeae

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Funds:

国家自然科学基金资助项目（30901155）; 浙江省自然科学基金资助项目（Y307499）

Received Date: 2015-03-04

Rev Recd Date: 2015-04-02

Publish Date: 2016-02-20

Key words:

Abstract: To study the structure and single nucleotide polymorphism （SNP） of non- 14-3-3 in Shibataeeae, homologue genes from five bamboo species, Hibanobambusa tranquillans, Indosasa shibataeaoides, Phyllostachys edulis, Shibataea chinensis, and Sinobambusa tootsik were cloned with gDNA and cDNA sequences being compared. Results showed five exons and four introns with CDS sequence lengths （in bp） of 789, 771, 789, and 786, respectively. There were 21, 19, 26, and 17 SNP sites in 14-3-3b, 14-3-3c, 14-3-3e, and 14-3-3f coding regions, with SNP frequencies （in bp） of 1SNP/38, 1SNP/41, 1SNP/30, and 1SNP/46, respectively. Nucleotide diversity （） for SNPs was 0.015 21, 0.012 17, 0.010 98, and 0.009 16, in that order. The amino acid sequence identity for these same genes in different species was 99%. Four 14-3-3 putative proteins were hydrophilic, and their isoelectric point was distributed from 4.75 to 4.90 with the amino acids Ala, Ser, and Gly being abundant. Also, -helixs and random coils in these proteins were the most plentiful; whereas, -turns and extended strands were few. These results lay a foundation for deeper studies with biological functions of 14-3-3 in bamboo. ［Ch, 3 fig. 3 tab. 27 ref.］

Single nucleotide polymorphism of non-ε 14-3-3 genes in Shibataeeae

doi: 10.11833/j.issn.2095-0756.2016.01.011

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

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