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摘要: 通过观测尾叶紫薇Lagerstroemia caudate花朵的开放过程,并采用离体萌发法测定花粉生活力,研究了尾叶紫薇的开花习性、花粉萌发及储藏条件。结果表明:凌晨3:30尾叶紫薇花萼开裂;6:00花瓣全部展平;6:30花药开始开裂,柱头上开始分泌黏液,进行杂交育种时须于6:30前去雄;6:30-7:30是授粉的最佳时间。采用正交试验方法比较蔗糖、聚乙二醇4000(PEG 4000)、硼酸和氯化钙对尾叶紫薇花粉的离体萌发率的影响。结果表明:最佳离体培养基为100 mgL-1蔗糖+30 mgL-1硼酸+30 mgL-1氯化钙+150 gL-1 PEG 4000,花粉萌发率可达96.28%。尾叶紫薇花粉在自然条件下储藏2 d后生活力急剧下降,6 d后花粉完全失活,4 ℃是花粉储藏的最佳温度,保存9 d仍能保持30%的萌发率,在散粉后30 d失活。图2表2参15Abstract: In order to study the flowering habit and the conditions for pollen germination and storage of Lagerstroemia caudate,the processes of flower opening and pollen viability of Lagerstroemia caudate were observed over a 30 d period. Pollen germination was also tested using orthogonal test of PEG 4000,boric acid,CaCl2,and sucrose. Results showed that at 3:30 sepals of L. caudate started to diverge,at 6:00 petals flattened up,at 6:30 the anther diverged,and at 06:30 the stigma secreted a large amount of mucus. The highest rate of in vitro pollen germination which could reach 96.28% was with sucrose (100 gL-1) + boric acid (30 mgL-1) + CaCl2 (30 mgL-1) + PEG 4000 (150 gL-1). Pollen germination in the indoor natural environment decreased sharply from 87% to 65% in 2 d with the pollen losing vigor in 6 d. The best preservation temperature for dry pollen was 4 ℃,in which temperature the dry pollen viability could keep 30%,with dry pollen losing its vigor in 30 d. Thus,as a basis for breeding,if L. caudate was used as a female parent in hybridization,the stamens must be removed before 6:30,and the best pollination time would be at 7:30.[Ch,2 fig. 2 tab. 15 ref.]
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Key words:
- botany /
- Lagerstroemia caudate /
- flowering habit /
- pollen germination /
- pollen storage
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2012.06.024

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