Effect of NaCl stress on the morphology and related physiological indexes of Magnolia biondii seedlings
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摘要:
目的 探讨盐胁迫对望春玉兰Magnolia biondii幼苗形态和相关生理指标的影响，并筛选盐胁迫下的指示性生理指标，为木兰科Magnoliaceae植物的耐盐性评价与筛选优质种质资源提供理论依据。 方法 以望春玉兰1年生实生苗为材料，采用水培法，测定200 mmol·L−1氯化钠胁迫下叶片的相对电导率、丙二醛质量摩尔浓度、叶绿素质量分数、渗透调节物质质量分数和抗氧化酶活性，通过主成分分析筛选关键生理指标。 结果 随着盐胁迫时间的延长，可溶性糖质量分数、相对电导率、丙二醛质量摩尔浓度和超氧化物歧化酶(SOD)活性总体呈上升趋势；可溶性蛋白质质量分数、脯氨酸质量分数、叶绿素质量分数和过氧化物酶(POD)活性总体呈先上升后下降的趋势。利用主成分分析法筛选出3个指示性指标，分别为丙二醛质量摩尔浓度、可溶性糖质量分数和SOD活性。 结论 氯化钠胁迫引起望春玉兰幼苗叶片丙二醛质量摩尔浓度、可溶性糖质量分数和SOD活性显著变化，可作为指示性生理指标用于评价氯化钠胁迫下望春玉兰等木兰科植物的耐盐性强弱。图4表2参19 Abstract:Objective With an investigation conducted of the effects of NaCl stress on the morphology and related physiological indexes of Magnolia biondii seedlings and the physiological indexes indicating the NaCl stress screened, this paper is aimed to provide a theoretical basis for the evaluation of the salt tolerance of Magnoliaceae and the selection of high-quality germplasm resources. Method Firstly, the hydroponic culture experiment was done with M. biondii 1-year-old seedlings with the effects of NaCl stress under 200 mmol·L−1 on relative electrical conductivity, MDA contents, chlorophyll contents, osmotic substances and antioxidant enzyme activities in leaves tested, after which the principal component analysis was conducted to screen key physiological indexes. Result With the increase of treatment time, the soluble sugar content, relative electric conductivity, MDA content and SOD activity increased. The soluble protein content, Pro content, chlorophyll content and POD activity first increased and then decreased. The three indicative physiological indexes screened by principal component analysis were MDA content, soluble sugar content and SOD activity. Conclusion The MDA content, soluble sugar content and SOD activity underwent significant changes under NaCl stress, and they could be used as key physiological indexes in the evaluation of the salt tolerance of Magnoliaceae such as M. biondii. [Ch, 4 fig. 2 tab. 19 ref.] -
Key words:
- botany /
- Magnolia biondii /
- NaCl stress /
- physiology responses /
- principal component analysis /
- indicative indexes
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图 1 盐胁迫下望春玉兰受害症状调查
Figure 1 Survey on the symptoms of salt damage of M. biondii under NaCl stress
图 2 盐胁迫下叶片可溶性糖、可溶性蛋白质和脯氨酸的变化
Figure 2 Changes of soluble sugar content, soluble protein content and Pro content in leaves under NaCl stress
图 3 盐胁迫下叶片叶绿素、相对电导率和丙二醛的变化
Figure 3 Changes of chlorophyll content, relative conductivity and MDA content in leaves under NaCl stress
图 4 盐胁迫下叶片SOD和POD活性的变化
Figure 4 Changes of SOD activity and POD activity in leaves under NaCl stress
表 1 盐胁迫下望春玉兰叶片生理指标的相关系数
Table 1. Correlation matrix of physiological indexes of M. biondii under NaCl stress
相对电导率 丙二醛 叶绿素 可溶性糖 可溶性蛋白 脯氨酸 SOD POD 相对电导率 1 丙二醛 0.868 1 叶绿素 −0.071 0.297 1 可溶性糖 0.906* 0.966** 0.100 1 可溶性蛋白 0.260 0.139 −0.090 0.002 1 脯氨酸 0.006 0.255 0.484 0.305 −0.873 1 SOD 0.502 0.818 0.460 0.680 0.356 0.047 1 POD 0.019 0.407 0.982** 0.238 −0.205 0.610 0.512 1 说明：*表示差异显著(P＜0.05)，**表示差异极显著(P＜0.01) 
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表 2 盐胁迫下望春玉兰叶片生理指标的主成分分析
Table 2. Principal component analysis of physiological indexes of M. biondii under NaCl stress
指标 主成分1 主成分2 指标 主成分1 主成分2 相对电导率 0.721 0.549 脯氨酸 0.425 −0.793 丙二醛 0.958 0.260 超氧化物歧化酶 0.837 0.207 叶绿素 0.549 −0.616 过氧化物酶 0.651 −0.640 可溶性糖 0.882 0.292 特征值 3.821 2.491 可溶性蛋白 0.023 0.759 贡献率/% 47.758 31.134
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