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高效降解菌Arthrobacter nicotianae ZAF-05对土霉素的减毒效应

朱蓉蓉 张赞 史艳可 林辉 张昕

朱蓉蓉, 张赞, 史艳可, 林辉, 张昕. 高效降解菌Arthrobacter nicotianae ZAF-05对土霉素的减毒效应[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20200819
引用本文: 朱蓉蓉, 张赞, 史艳可, 林辉, 张昕. 高效降解菌Arthrobacter nicotianae ZAF-05对土霉素的减毒效应[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20200819
ZHU Rongrong, ZHANG Zan, SHI Yanke, LIN Hui, ZHANG Xin. Reduction of toxicity of oxytetracycline by highly efficient degrading bacterium Arthrobacter nicotianae ZAF-05[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20200819
Citation: ZHU Rongrong, ZHANG Zan, SHI Yanke, LIN Hui, ZHANG Xin. Reduction of toxicity of oxytetracycline by highly efficient degrading bacterium Arthrobacter nicotianae ZAF-05[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20200819

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高效降解菌Arthrobacter nicotianae ZAF-05对土霉素的减毒效应

doi: 10.11833/j.issn.2095-0756.20200819
基金项目: 国家自然科学基金资助项目(31970093,31670110)
详细信息
    作者简介: 朱蓉蓉(ORCID: 00000-0002-7487-6350),从事微生物生态学相关研究。E-mail: 734632819@qq.com
    通信作者: 张昕(ORCID: 0000-0002-8762-171X),副教授,从事环境微生物学相关研究,E-mail: zhangxins@126.com
  • 中图分类号: S606

Reduction of toxicity of oxytetracycline by highly efficient degrading bacterium Arthrobacter nicotianae ZAF-05

  • 摘要:   目的  明确1株土霉素(oxytetracycline, OTC)高效降解菌降低OTC生物毒性的效应,为菌株的实际应用提供初步理论依据。  方法  以前期获得的OTC高效降解菌Arthrobacter nicotianae ZAF-05为对象,利用高效液相色谱-电喷雾质谱联用技术(HPLC-Q-TOF-MS)分析了OTC的降解产物。以对OTC敏感的大肠埃希菌Escherichia coli、枯草芽孢杆菌Bacillus subtilis和斜生栅藻Scenedesmus obliquus作为指示生物,评估OTC降解产物的生物毒性。  结果  HPLC-Q-TOF-MS分析发现:经菌株ZAF-05作用后,体系中的高毒性OTC以及其自然水解产物差向异构土霉素(EOTC)含量显著降低,而低毒性的同分异构土霉素(ISO-OTC)含量略有上升。供试细菌和藻类在添加ZAF-05菌株的OTC体系中的生长情况明显好于OTC自然水解的处理和未添加降解菌的阴性对照,且差异极显著(P<0.01)。与原药和自然水解物相比,经过ZAF-05菌株降解的OTC代谢产物生物毒性大大降低。菌株的降解作用缓解了OTC对斜生栅藻细胞超微结构的伤害,细胞叶绿体及细胞壁受损减轻。  结论  降解菌ZAF-05能降低OTC残留的环境生物毒性,而毒性的降低可能与体系中高毒性物质被转化成无毒或低毒的代谢产物有关。图7表1参26
  • 图  1  TP447的色谱和质谱图

    Figure  1  Extracted ion chromatograms and mass spectrum of TP447

    图  2  TP461的色谱和质谱图

    Figure  2  Extracted ion chromatograms and mass spectrum of TP461

    图  3  不同处理对枯草芽孢杆菌生长的影响

    Figure  3  Effects of different treatments on the growth of B. subtilis

    图  4  不同处理对大肠埃希菌生长的影响

    Figure  4  Effects of different treatments on the growth of E. coli

    图  5  不同处理对斜生栅藻生长的影响

    Figure  5  Effects of different treatments on the growth of S. obliquus

    图  6  不同处理对斜生栅藻叶绿素的影响

    Figure  6  Effects of different treatments on the chlorophyll content of S. obliquus

    图  7  不同处理下斜生栅藻的亚细胞形态

    Figure  7  Effects of different treatments on the chlorophyll content of S. obliquus

    表  1  OTC及其推定代谢产物的相对丰度

    Table  1.   Relative abundance of OTC and the putative degradation products      

    可能的化合物保留时间/min分子式相对丰度
    阳性对照降解菌处理
    OTC 5.434 C22H24N2O9 13.872 7.254
    EOTC 4.893 C22H24N2O9 20.931 5.231
    ISO-OTC 6.550 C22H24N2O9 2.727 3.157
    脱甲基OTC 7.279 C21H22N2O9 37.278 4.142
    下载: 导出CSV
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  • 收稿日期:  2021-01-08
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高效降解菌Arthrobacter nicotianae ZAF-05对土霉素的减毒效应

doi: 10.11833/j.issn.2095-0756.20200819
    基金项目:  国家自然科学基金资助项目(31970093,31670110)
    作者简介:

    朱蓉蓉(ORCID: 00000-0002-7487-6350),从事微生物生态学相关研究。E-mail: 734632819@qq.com

    通信作者: 张昕(ORCID: 0000-0002-8762-171X),副教授,从事环境微生物学相关研究,E-mail: zhangxins@126.com
  • 中图分类号: S606

摘要:   目的  明确1株土霉素(oxytetracycline, OTC)高效降解菌降低OTC生物毒性的效应,为菌株的实际应用提供初步理论依据。  方法  以前期获得的OTC高效降解菌Arthrobacter nicotianae ZAF-05为对象,利用高效液相色谱-电喷雾质谱联用技术(HPLC-Q-TOF-MS)分析了OTC的降解产物。以对OTC敏感的大肠埃希菌Escherichia coli、枯草芽孢杆菌Bacillus subtilis和斜生栅藻Scenedesmus obliquus作为指示生物,评估OTC降解产物的生物毒性。  结果  HPLC-Q-TOF-MS分析发现:经菌株ZAF-05作用后,体系中的高毒性OTC以及其自然水解产物差向异构土霉素(EOTC)含量显著降低,而低毒性的同分异构土霉素(ISO-OTC)含量略有上升。供试细菌和藻类在添加ZAF-05菌株的OTC体系中的生长情况明显好于OTC自然水解的处理和未添加降解菌的阴性对照,且差异极显著(P<0.01)。与原药和自然水解物相比,经过ZAF-05菌株降解的OTC代谢产物生物毒性大大降低。菌株的降解作用缓解了OTC对斜生栅藻细胞超微结构的伤害,细胞叶绿体及细胞壁受损减轻。  结论  降解菌ZAF-05能降低OTC残留的环境生物毒性,而毒性的降低可能与体系中高毒性物质被转化成无毒或低毒的代谢产物有关。图7表1参26

English Abstract

朱蓉蓉, 张赞, 史艳可, 林辉, 张昕. 高效降解菌Arthrobacter nicotianae ZAF-05对土霉素的减毒效应[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20200819
引用本文: 朱蓉蓉, 张赞, 史艳可, 林辉, 张昕. 高效降解菌Arthrobacter nicotianae ZAF-05对土霉素的减毒效应[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20200819
ZHU Rongrong, ZHANG Zan, SHI Yanke, LIN Hui, ZHANG Xin. Reduction of toxicity of oxytetracycline by highly efficient degrading bacterium Arthrobacter nicotianae ZAF-05[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20200819
Citation: ZHU Rongrong, ZHANG Zan, SHI Yanke, LIN Hui, ZHANG Xin. Reduction of toxicity of oxytetracycline by highly efficient degrading bacterium Arthrobacter nicotianae ZAF-05[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20200819

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