日本扁柏部分双列杂交试验的RAPD遗传分析

汤定钦; 井上诚; 近藤明; 井出雄二

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日本扁柏部分双列杂交试验的RAPD遗传分析

汤定钦 , , 井上诚 , 近藤明 , 井出雄二

文章导航 > 浙江农林大学学报 > 2004 > 21(3): 235-242

汤定钦, 井上诚, 近藤明, 井出雄二. 日本扁柏部分双列杂交试验的RAPD遗传分析[J]. 浙江农林大学学报, 2004, 21(3): 235-242.

引用本文:

汤定钦, 井上诚, 近藤明, 井出雄二. 日本扁柏部分双列杂交试验的RAPD遗传分析[J]. 浙江农林大学学报, 2004, 21(3): 235-242.

TANG Ding-qin, INOUE Makoto, KONDO Akira, IDE Yuji. Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test[J]. Journal of Zhejiang A&F University, 2004, 21(3): 235-242.

Citation:

TANG Ding-qin, INOUE Makoto, KONDO Akira, IDE Yuji. Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test[J]. Journal of Zhejiang A&F University, 2004, 21(3): 235-242.

日本扁柏部分双列杂交试验的RAPD遗传分析

1.
浙江林学院浙江省现代森林培育技术重点实验室, 浙江临安 311300;
2.
东京大学爱知演示林, 东京 113;
3.
静冈县林科所, 静冈滨北;
4.
东京大学农学生命科学研究科, 东京 113

基金项目:

This research was supported by a grant-in-aid No .07556035 of the Ministry of Education Science and Culture of Japan .

详细信息

作者简介: TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn

中图分类号: S718.46 ;Q943

Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test

1.
.Key Lab forModern Silvicultural Technology of Zhejiang Province, Zhejiang Forestry College, Lin’an 311300 , Zhejiang , China;
2.
.University Forest in Aichi , The University Forests , Faculty of Agriculture , The University of Tokyo , Tokyo 1 1 3 , Japan;
3.
.Shizuoka Prefecture Forest ry and Forest Products Research Institute , Hamakita 4 3 7 , Negata , Japan;
4.
.Graduate S chool of Agricultural and Life Sciences , The University of Tokyo, Tokyo 113 , Japan

Funds:

This research was supported by a grant-in-aid No .07556035 of the Ministry of Education Science and Culture of Japan .

More Information

Author Bio:
TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn

TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn

摘要: RAPD 作为一简易的DNA 分子标记, 在得以广泛应用的同时, 存在着随机扩增的DNA 条带并不遵行显性遗传模式, 以及受PCR 反应条件影响大等现象。以5 个日本扁柏Chamaecyparis obtusa 优树无性系及其部分双列杂交的12 组合(各含30 个体)F1 代为材料, 探讨RAPD 标记的子代遗传及分离特征。研究结果表明:优树无性系中供试的46 个引物中, 14 个引物扩增了42 条多态的片段, 筛选了其中7 个引物扩增得来的14 条片段进行分析, 在子代中均能找到其对应的片段, 且这些片段符合孟德尔的遗传分离规律。说明RAPD 可作为遗传标记在日本扁柏中用于遗传分析。图1 表2 参18
- 日本扁柏 /
- 部分双列杂交 /
- RAPD /
- 分离分析
Abstract: To develop molecular markers for Chamaecyparis obtusa , the inheritance of RAPD fragments was studied in diploid tissues of 5 parents and 30 diploid controlled F1 progenies in each partial diallel crosses of these parents .Of 46 primers tested , 18 primers yielded 135 reproducible fragments , of which 42 fragments (31 %)were polymorphic among the parents .Fourteen fragments amplified from 7 primers were selected to test the segregation among controlled F1 progenies .All fragments observed in the parents were found in the progeny .Segregation of all variable fragments observed in diploid materials fitted the proportions expected for a dominant Mendelian trait .The segregating fragments were either present or absent confirming the dominant character of RAPD variation .The usefulness of RAPD fragments as genetic markers for estimating genetic diversity was also discussed .[ En , 1 fig .2 tab .18 ref .]
- Chamaecyparis obtusa /
- diallel crosses /
- RAPD /
- segregation analysis

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日本扁柏部分双列杂交试验的RAPD遗传分析

1. 浙江林学院浙江省现代森林培育技术重点实验室, 浙江临安 311300;

2. 东京大学爱知演示林, 东京 113;

3. 静冈县林科所, 静冈滨北;

4. 东京大学农学生命科学研究科, 东京 113

基金项目:

This research was supported by a grant-in-aid No .07556035 of the Ministry of Education Science and Culture of Japan .

作者简介:
TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn

收稿日期: 2004-03-16
修回日期: 2004-05-19
刊出日期: 2004-09-20

中图分类号: S718.46 ;Q943

关键词:

摘要: RAPD 作为一简易的DNA 分子标记, 在得以广泛应用的同时, 存在着随机扩增的DNA 条带并不遵行显性遗传模式, 以及受PCR 反应条件影响大等现象。以5 个日本扁柏Chamaecyparis obtusa 优树无性系及其部分双列杂交的12 组合(各含30 个体)F1 代为材料, 探讨RAPD 标记的子代遗传及分离特征。研究结果表明:优树无性系中供试的46 个引物中, 14 个引物扩增了42 条多态的片段, 筛选了其中7 个引物扩增得来的14 条片段进行分析, 在子代中均能找到其对应的片段, 且这些片段符合孟德尔的遗传分离规律。说明RAPD 可作为遗传标记在日本扁柏中用于遗传分析。图1 表2 参18

English Abstract

Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test

1. .Key Lab forModern Silvicultural Technology of Zhejiang Province, Zhejiang Forestry College, Lin’an 311300 , Zhejiang , China;

2. .University Forest in Aichi , The University Forests , Faculty of Agriculture , The University of Tokyo , Tokyo 1 1 3 , Japan;

3. .Shizuoka Prefecture Forest ry and Forest Products Research Institute , Hamakita 4 3 7 , Negata , Japan;

4. .Graduate S chool of Agricultural and Life Sciences , The University of Tokyo, Tokyo 113 , Japan

Funds:

This research was supported by a grant-in-aid No .07556035 of the Ministry of Education Science and Culture of Japan .

Author Bio:
TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn

TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn

Received Date: 2004-03-16
Rev Recd Date: 2004-05-19
Publish Date: 2004-09-20

Keywords:

Abstract: To develop molecular markers for Chamaecyparis obtusa , the inheritance of RAPD fragments was studied in diploid tissues of 5 parents and 30 diploid controlled F1 progenies in each partial diallel crosses of these parents .Of 46 primers tested , 18 primers yielded 135 reproducible fragments , of which 42 fragments (31 %)were polymorphic among the parents .Fourteen fragments amplified from 7 primers were selected to test the segregation among controlled F1 progenies .All fragments observed in the parents were found in the progeny .Segregation of all variable fragments observed in diploid materials fitted the proportions expected for a dominant Mendelian trait .The segregating fragments were either present or absent confirming the dominant character of RAPD variation .The usefulness of RAPD fragments as genetic markers for estimating genetic diversity was also discussed .[ En , 1 fig .2 tab .18 ref .]

汤定钦, 井上诚, 近藤明, 井出雄二. 日本扁柏部分双列杂交试验的RAPD遗传分析[J]. 浙江农林大学学报, 2004, 21(3): 235-242.

引用本文:

汤定钦, 井上诚, 近藤明, 井出雄二. 日本扁柏部分双列杂交试验的RAPD遗传分析[J]. 浙江农林大学学报, 2004, 21(3): 235-242.

TANG Ding-qin, INOUE Makoto, KONDO Akira, IDE Yuji. Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test[J]. Journal of Zhejiang A&F University, 2004, 21(3): 235-242.

Citation:

TANG Ding-qin, INOUE Makoto, KONDO Akira, IDE Yuji. Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test[J]. Journal of Zhejiang A&F University, 2004, 21(3): 235-242.