日本扁柏部分双列杂交试验的RAPD遗传分析

汤定钦; 井上诚; 近藤明; 井出雄二

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

日本扁柏部分双列杂交试验的RAPD遗传分析

汤定钦 , , 井上诚 , 近藤明 , 井出雄二

文章导航 > 浙江农林大学学报 > 2004 > 21(3): 235-242

汤定钦, 井上诚, 近藤明, 井出雄二. 日本扁柏部分双列杂交试验的RAPD遗传分析[J]. 浙江农林大学学报, 2004, 21(3): 235-242.

引用本文:

汤定钦, 井上诚, 近藤明, 井出雄二. 日本扁柏部分双列杂交试验的RAPD遗传分析[J]. 浙江农林大学学报, 2004, 21(3): 235-242.

TANG Ding-qin, INOUE Makoto, KONDO Akira, IDE Yuji. Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test[J]. Journal of Zhejiang A&F University, 2004, 21(3): 235-242.

Citation:

TANG Ding-qin, INOUE Makoto, KONDO Akira, IDE Yuji. Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test[J]. Journal of Zhejiang A&F University, 2004, 21(3): 235-242.

日本扁柏部分双列杂交试验的RAPD遗传分析

1.
浙江林学院浙江省现代森林培育技术重点实验室, 浙江临安 311300;
2.
东京大学爱知演示林, 东京 113;
3.
静冈县林科所, 静冈滨北;
4.
东京大学农学生命科学研究科, 东京 113

基金项目:

This research was supported by a grant-in-aid No .07556035 of the Ministry of Education Science and Culture of Japan .

详细信息

作者简介: TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn

中图分类号: S718.46 ;Q943

Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test

1.
.Key Lab forModern Silvicultural Technology of Zhejiang Province, Zhejiang Forestry College, Lin’an 311300 , Zhejiang , China;
2.
.University Forest in Aichi , The University Forests , Faculty of Agriculture , The University of Tokyo , Tokyo 1 1 3 , Japan;
3.
.Shizuoka Prefecture Forest ry and Forest Products Research Institute , Hamakita 4 3 7 , Negata , Japan;
4.
.Graduate S chool of Agricultural and Life Sciences , The University of Tokyo, Tokyo 113 , Japan

Funds:

This research was supported by a grant-in-aid No .07556035 of the Ministry of Education Science and Culture of Japan .

More Information

Author Bio:
TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn

TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn

摘要: RAPD 作为一简易的DNA 分子标记, 在得以广泛应用的同时, 存在着随机扩增的DNA 条带并不遵行显性遗传模式, 以及受PCR 反应条件影响大等现象。以5 个日本扁柏Chamaecyparis obtusa 优树无性系及其部分双列杂交的12 组合(各含30 个体)F1 代为材料, 探讨RAPD 标记的子代遗传及分离特征。研究结果表明:优树无性系中供试的46 个引物中, 14 个引物扩增了42 条多态的片段, 筛选了其中7 个引物扩增得来的14 条片段进行分析, 在子代中均能找到其对应的片段, 且这些片段符合孟德尔的遗传分离规律。说明RAPD 可作为遗传标记在日本扁柏中用于遗传分析。图1 表2 参18
- 日本扁柏 /
- 部分双列杂交 /
- RAPD /
- 分离分析
Abstract: To develop molecular markers for Chamaecyparis obtusa , the inheritance of RAPD fragments was studied in diploid tissues of 5 parents and 30 diploid controlled F1 progenies in each partial diallel crosses of these parents .Of 46 primers tested , 18 primers yielded 135 reproducible fragments , of which 42 fragments (31 %)were polymorphic among the parents .Fourteen fragments amplified from 7 primers were selected to test the segregation among controlled F1 progenies .All fragments observed in the parents were found in the progeny .Segregation of all variable fragments observed in diploid materials fitted the proportions expected for a dominant Mendelian trait .The segregating fragments were either present or absent confirming the dominant character of RAPD variation .The usefulness of RAPD fragments as genetic markers for estimating genetic diversity was also discussed .[ En , 1 fig .2 tab .18 ref .]
- Chamaecyparis obtusa /
- diallel crosses /
- RAPD /
- segregation analysis

[1]	王安妮, 王开良, 柴静瑜, 钟慧奇, 滕建华, 张磊, 俞文仙, 林萍. 普通油茶全双列杂交子代种仁含油率及其相关性状的遗传分析 . 浙江农林大学学报, 2024, 41(6): 1170-1179. doi: 10.11833/j.issn.2095-0756.20230603
[2]	骆嘉言, 王英, 钟文翰, 刘海冲, 蒋火俊. 樟科16个树种木材的RAPD与ISSR分子鉴别 . 浙江农林大学学报, 2017, 34(5): 942-948. doi: 10.11833/j.issn.2095-0756.2017.05.023
[3]	阮佳佳, 袁进强, 杨仙玉. 日本蟾蜍皮肤claudin-4 cDNA的克隆与序列分析 . 浙江农林大学学报, 2013, 30(4): 561-566. doi: 10.11833/j.issn.2095-0756.2013.04.016
[4]	宋敏国, 袁进强, 杨仙玉, 张姝芳, 诸葛慧, 徐跃. 日本蟾蜍皮肤胸腺素α原cDNA的克隆及序列分析 . 浙江农林大学学报, 2013, 30(3): 401-405. doi: 10.11833/j.issn.2095-0756.2013.03.016
[5]	张振, 张含国, 张磊, 朱航勇, 李雪峰. 兴安落叶松基本群体与育种群体RAPD多样性分析 . 浙江农林大学学报, 2012, 29(1): 130-136. doi: 10.11833/j.issn.2095-0756.2012.01.022
[6]	高燕会, 樊民亮, 骆文坚, 黄华宏, 童再康. 濒危树种金钱松RAPD体系的建立和遗传多样性分析 . 浙江农林大学学报, 2011, 28(5): 815-822. doi: 10.11833/j.issn.2095-0756.2011.05.023
[7]	蓝云龙, 吴令上, 裘波音, 高燕会, 斯金平. 鱼腥草RAPD分子标记的多态性 . 浙江农林大学学报, 2008, 25(3): 309-313.
[8]	曹福亮, 花喆斌, 汪贵斌, 张往祥. 野生银杏资源群体遗传多样性的RAPD分析 . 浙江农林大学学报, 2008, 25(1): 22-27.
[9]	王祖良, 丁丽霞, 赵明水, 程晓渊, 沈乾. 濒危植物天目铁木遗传多样性的RAPD分析 . 浙江农林大学学报, 2008, 25(3): 304-308.
[10]	杨敬元, 熊丹, 梁宏伟, 王玉兵, 廖明尧, 陈发菊. 珍稀濒危植物香果树RAPD反应条件的优化 . 浙江农林大学学报, 2007, 24(3): 279-283.
[11]	杨毅, 周祖基, 张立钦, 马良进, 林新春, 高洁. 寄生松墨天牛的球孢白僵菌不同菌株DNA多态性的RAPD 分析 . 浙江农林大学学报, 2005, 22(3): 306-309.
[12]	曾燕如, 胡瑛, 斯金平, 徐象华. 浙江丽水16 个柑橘特色品种的RAPD 分析 . 浙江农林大学学报, 2005, 22(5): 481-485.
[13]	程晓建, 杨萍, 林伯年, 郑炳松. 应用RAPD 技术探讨红叶李、李和杏的亲缘关系 . 浙江农林大学学报, 2004, 21(1): 40-43.
[14]	王正加, 黄坚钦, 郭传友, 杨萍, 王华芳. 山核桃RAPD 反应体系的优化 . 浙江农林大学学报, 2003, 20(4): 429-433.
[15]	金则新, 李钧敏, 钟章成. 大血藤RAPD 条件的优化 . 浙江农林大学学报, 2003, 20(2): 141-145.
[16]	雷日平, 陈辉, 谢利国. 锥栗不同品种遗传距离的RAPD 分析 . 浙江农林大学学报, 2002, 19(3): 240-243.
[17]	方伟, 何祯祥, 黄坚钦, 史钱均, 林新春. 雷竹不同栽培类型RAPD 分子标记的研究 . 浙江农林大学学报, 2001, 18(1): 1-5.
[18]	张立钦, 林新春, 毛胜凤. 绿僵菌不同菌株DNA 多态性的RAPD 分析 . 浙江农林大学学报, 2001, 18(3): 281-285.
[19]	梁月荣, 田中淳一, 武田善行. 茶树品种资源遗传多态性RAPD 分析 . 浙江农林大学学报, 2000, 17(2): 215-218.
[20]	张建章, 游昌顺, 吕锦松. 杉木半双列杂交后代生长性状的配合力分析 . 浙江农林大学学报, 1998, 15(2): 138-144.

链接本文:
https://zlxb.zafu.edu.cn/article/id/1673

https://zlxb.zafu.edu.cn/article/zjnldxxb/2004/3/235

点击查看大图

计量

文章访问数: 1144
HTML全文浏览量: 123
PDF下载量: 193
被引次数: 0

日本扁柏部分双列杂交试验的RAPD遗传分析

1. 浙江林学院浙江省现代森林培育技术重点实验室, 浙江临安 311300;

2. 东京大学爱知演示林, 东京 113;

3. 静冈县林科所, 静冈滨北;

4. 东京大学农学生命科学研究科, 东京 113

基金项目:

This research was supported by a grant-in-aid No .07556035 of the Ministry of Education Science and Culture of Japan .

作者简介:
TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn

收稿日期: 2004-03-16
修回日期: 2004-05-19
刊出日期: 2004-09-20

中图分类号: S718.46 ;Q943

关键词:

摘要: RAPD 作为一简易的DNA 分子标记, 在得以广泛应用的同时, 存在着随机扩增的DNA 条带并不遵行显性遗传模式, 以及受PCR 反应条件影响大等现象。以5 个日本扁柏Chamaecyparis obtusa 优树无性系及其部分双列杂交的12 组合(各含30 个体)F1 代为材料, 探讨RAPD 标记的子代遗传及分离特征。研究结果表明:优树无性系中供试的46 个引物中, 14 个引物扩增了42 条多态的片段, 筛选了其中7 个引物扩增得来的14 条片段进行分析, 在子代中均能找到其对应的片段, 且这些片段符合孟德尔的遗传分离规律。说明RAPD 可作为遗传标记在日本扁柏中用于遗传分析。图1 表2 参18

English Abstract

Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test

1. .Key Lab forModern Silvicultural Technology of Zhejiang Province, Zhejiang Forestry College, Lin’an 311300 , Zhejiang , China;

2. .University Forest in Aichi , The University Forests , Faculty of Agriculture , The University of Tokyo , Tokyo 1 1 3 , Japan;

3. .Shizuoka Prefecture Forest ry and Forest Products Research Institute , Hamakita 4 3 7 , Negata , Japan;

4. .Graduate S chool of Agricultural and Life Sciences , The University of Tokyo, Tokyo 113 , Japan

Funds:

This research was supported by a grant-in-aid No .07556035 of the Ministry of Education Science and Culture of Japan .

Author Bio:
TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn

TANG Ding-qin (1966 -), male , born in Dongshan of Fujian Province , received hi s Ph D degree from the University of Tokyo , Japan, and his postdoctoral training was at the Asian Natural Resource &Environmental Center , the University of Tokyo, Japan .Dr TANG is current ly a specially appointed prof essor of Zhejiang Forestry College, China.E-mail:tang@zjfc.edu.cn

Received Date: 2004-03-16
Rev Recd Date: 2004-05-19
Publish Date: 2004-09-20

Keywords:

Abstract: To develop molecular markers for Chamaecyparis obtusa , the inheritance of RAPD fragments was studied in diploid tissues of 5 parents and 30 diploid controlled F1 progenies in each partial diallel crosses of these parents .Of 46 primers tested , 18 primers yielded 135 reproducible fragments , of which 42 fragments (31 %)were polymorphic among the parents .Fourteen fragments amplified from 7 primers were selected to test the segregation among controlled F1 progenies .All fragments observed in the parents were found in the progeny .Segregation of all variable fragments observed in diploid materials fitted the proportions expected for a dominant Mendelian trait .The segregating fragments were either present or absent confirming the dominant character of RAPD variation .The usefulness of RAPD fragments as genetic markers for estimating genetic diversity was also discussed .[ En , 1 fig .2 tab .18 ref .]

汤定钦, 井上诚, 近藤明, 井出雄二. 日本扁柏部分双列杂交试验的RAPD遗传分析[J]. 浙江农林大学学报, 2004, 21(3): 235-242.

引用本文:

汤定钦, 井上诚, 近藤明, 井出雄二. 日本扁柏部分双列杂交试验的RAPD遗传分析[J]. 浙江农林大学学报, 2004, 21(3): 235-242.

TANG Ding-qin, INOUE Makoto, KONDO Akira, IDE Yuji. Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test[J]. Journal of Zhejiang A&F University, 2004, 21(3): 235-242.

Citation:

TANG Ding-qin, INOUE Makoto, KONDO Akira, IDE Yuji. Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test[J]. Journal of Zhejiang A&F University, 2004, 21(3): 235-242.