Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture
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摘要: 以南方红豆杉Taxus chinensis var. mairei成熟胚为外植体,筛选出外植体灭菌途径,研究了基本培养基、生长调节剂等因子对其离体胚生长的影响。结果表明:最优灭菌处理为25.0 gL-1的次氯酸钠(NaClO)溶液真空灭菌10 min,其污染率仅为6%,出苗率可达86%;木本植物培养剂(WPM)为胚培养的最适基本培养基;细胞激动素(KT) 0.1 mgL-1和玉米素(ZT)1.0 mgL-1最有效于不定芽的诱导,不定芽为丛生芽;6-苄氨基腺嘌呤(6-BA)和异戊烯基腺嘌呤(2iP)诱导不定芽较少;噻苯隆(TDZ)不能诱导不定芽;以萌动种胚为外植体对不定芽的诱导优于未萌动种胚。图3表7参11Abstract: The effect of seed sterilization conditions,various basal media,and growth regulators on explants of mature Taxus chinensis var. mairei embryos were studied. Results showed optimal germination occurred with seeds soaked in a 25.0 gL-1 sodium hypochlorite solution for 10 min in a vacuum. The contamination rate was reduced to 6% and the germination rate reached 86%. A woody plant medium (WPM) was selected as the best basal medium with 0.1 mgL-1 kinetin (KT) and 1.0 mgL-1 zeatin (ZT). This medium effectively induced more clusters of adventitious bud formation;whereas 6-benzylaminopurine(6-BA) and isopenlenyladenine(2iP) induced fewer adventitious buds while N-phenyl-N-1,2,3-thidiazol-5-ylurea(TDZ) produced no adventitious bud differentiation. With WPM,germinated embryos effectively induced adventitious buds.[Ch,3 fig. 7 tab. 11 ref.]
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https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2010.04.023
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