Construction of a fat metabolism-related cDNA library in <em>Carya cathayensis</em>

HUANG Yin-zhi; ZHOU Qin; HUANG You-jun; ZENG Yan-ru

doi:10.11833/j.issn.2095-0756.2011.01.013

Volume 28 Issue 1

Feb. 2011

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2011 > 28(1): 80-85

HUANG Yin-zhi, ZHOU Qin, HUANG You-jun, ZENG Yan-ru. Construction of a fat metabolism-related cDNA library in Carya cathayensis[J]. Journal of Zhejiang A&F University, 2011, 28(1): 80-85. doi: 10.11833/j.issn.2095-0756.2011.01.013

Citation:

HUANG Yin-zhi, ZHOU Qin, HUANG You-jun, ZENG Yan-ru. Construction of a fat metabolism-related cDNA library in Carya cathayensis[J]. Journal of Zhejiang A&F University, 2011, 28(1): 80-85. doi: 10.11833/j.issn.2095-0756.2011.01.013

Construction of a fat metabolism-related cDNA library in Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2011.01.013

1.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang ，China

More Information

Corresponding author: ZENG Yan-ru
Publish Date: 2011-02-20

Abstract

Carya cathayensis is a species with a high oil content in its nuts. In addition，its oil is mostly unsaturated fatty acids. Based on differential display of genes during fruit maturity studied by DDRT-PCR，a fat metabolism-related cDNA library was constructed by SMART. Titering the unamplified library showed a titer of 5.0 105 pfumL－1，an insert size between 0.7 and 2.5 kb and 70.0% of inserts larger than 1.0 kb；and the amplified library had a titer of 5.0 109 pfumL－1，all of the inserts larger than 500 bp，and a recombination rate of 94.5% with 66.0% of the inserts longer than 1 000 bp. This work has laid a foundation for studies of fat-metabolizing mechanism and regulatory network as well as discovery of relevant genes.［Ch，7 fig. 19 ref.］
- cash forestry,
- Carya cathayensis,
- cDNA library,
- SMART,
- fat metabolism

Relative Article

[1]	HE Guoqing, YU Chunlian, RAO Ying, ZHANG Fuyang, SHEN Xiaofei, HUANG Jianqin, LIU Li, XIA Guohua. Dynamic changes in composition of mineral elements and fatty acids for hickory nuts (Carya cathayensis) during maturity . Journal of Zhejiang A&F University, 2019, 36(6): 1208-1216. doi: 10.11833/j.issn.2095-0756.2019.06.019
[2]	MA Shanshan, ZHAO Keli, DING Lizhong, HUANG Sha, CAI Ling, ZHAO Weiming, YE Zhengqian. Soil fertility in Carya cathayensis orchards for major towns of Lin'an City, China . Journal of Zhejiang A&F University, 2016, 33(6): 953-960. doi: 10.11833/j.issn.2095-0756.2016.06.005
[3]	HOU Chuanming, ZHENG Yawen, WANG Zhengjia, XU Yingwu. Cloning and analysis of a MADS-like gene in Carya cathayensis . Journal of Zhejiang A&F University, 2015, 32(1): 33-39. doi: 10.11833/j.issn.2095-0756.2015.01.005
[4]	HUANG Yinzhi, ZENG Yanru, ZHOU Qin, XIA Guohua, HUANG Youjun. Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis . Journal of Zhejiang A&F University, 2015, 32(2): 229-236. doi: 10.11833/j.issn.2095-0756.2015.02.009
[5]	SHEN Chen, QIU Jiani, HUANG Jianqin. Cloning and expression analysis of COP1 E3 ligase in Carya cathayensis . Journal of Zhejiang A&F University, 2014, 31(6): 831-837. doi: 10.11833/j.issn.2095-0756.2014.06.002
[6]	LI Shunfu, HU Hengkang, ZHANG Qiulu, XU Yanling, ZHANG Qixiang. ISSR analysis of DNA methylation variety on immature embryos of Carya cathayensis . Journal of Zhejiang A&F University, 2014, 31(4): 521-527. doi: 10.11833/j.issn.2095-0756.2014.04.005
[7]	WANG Xuan, MA Liangjin, LÜ Quan, MENG Xianjing, ZHANG Xingyao. Identification of the pathogens causing stem canker on Carya cathayensis . Journal of Zhejiang A&F University, 2014, 31(2): 238-245. doi: 10.11833/j.issn.2095-0756.2014.02.012
[8]	ZHU Hangrui, ZHUANG Xiaowei, PAN Xin, CHEN Shunwei, ZHANG Jiangli. Combustion performance of charcoal briquettes made from three kinds of epicarps . Journal of Zhejiang A&F University, 2013, 30(1): 90-94. doi: 10.11833/j.issn.2095-0756.2013.01.013
[9]	SONG Shuang, HUANG Yin-zhi, DONG Lei-ming, HUANG You-jun, ZENG Yan-ru, WU Zhi-min. Simple sequence repeat （SSR） markers in Carya cathayensis . Journal of Zhejiang A&F University, 2012, 29(4): 626-633. doi: 10.11833/j.issn.2095-0756.2012.04.023
[10]	YANG Jian-hua, LI Shu-fang, FAN Zhi-yuan, XI Xue-liang, ZOU Wei-lie, LIU Jiao, PAN Li. Principal component analysis for major economic characteristics of Carya illinoensis and selection of superior cultivars . Journal of Zhejiang A&F University, 2011, 28(6): 907-910. doi: 10.11833/j.issn.2095-0756.2011.06.011
[11]	LI Yuan-chun, SHEN Lin, ZENG Yan-ru. Establishment of a SRAP analysis protocol in Carya cathayensis and a comparison among SRAP，RAPD，ISSR analysis protocols . Journal of Zhejiang A&F University, 2011, 28(3): 505-512. doi: 10.11833/j.issn.2095-0756.2011.03.025
[12]	HU Heng-kang, JIANG Xiang-mei, ZHANG Qi-xiang, CHEN Bei, HUANG Jian-qin. Somatic embryogenesis and plant regeneration from Carya cathayensis embryos using different carbon sources . Journal of Zhejiang A&F University, 2011, 28(6): 911-917. doi: 10.11833/j.issn.2095-0756.2011.06.012
[13]	JIN Zhi-feng, ZHAO Hong-bo, LI Bo, LI Ren-zhong, HUANG Jing-feng. Optimum areas for Carya cathayensis planting in Zhejiang Province based on GIS . Journal of Zhejiang A&F University, 2011, 28(2): 256-261. doi: 10.11833/j.issn.2095-0756.2011.02.014
[14]	SONG Hong－gai, JIANG Jing, QIAO Gui－rong, YANG Ye, ZHOU Jing, PAN Luan－yin, ZHUO Ren－ying. Plant stress－resistant gene cloning for Na and Cd using an INVSc1 yeast . Journal of Zhejiang A&F University, 2010, 27(6): 890-895. doi: 10.11833/j.issn.2095-0756.2010.06.014
[15]	XU Yan, SHEN Yue-qin, HUANG Jian-qin, LIN Jian-hua. Farmers’ willingness to adopt ecological management model for Carya cathayensis . Journal of Zhejiang A&F University, 2010, 27(5): 750-756. doi: 10.11833/j.issn.2095-0756.2010.05.018
[16]	ZHENG Bing-song, CHEN Miao, CHU Huai-liang, AI Xue, HUANG You-jun, LI Xue-qin, HUANG Jian-qin. Auxin response factor fragments cloned by cDNA-AFLP during Carya cathayensis grafting process . Journal of Zhejiang A&F University, 2009, 26(4): 467-472.
[17]	HUANG You-jun, ZHOU Li, CHEN Fang-fang, ZHOU Qin, HUANG Jian-qin, HUANG Min-ren, WANG ming-xiu. Gene expression with cDNA-AFLP （amplified fragment length polymorphism） during flowering of Carya cathayensis . Journal of Zhejiang A&F University, 2009, 26(3): 297-301.
[18]	YANG Ye, JIANG Jing, QIAO Gui-rong, ZHOU Jing, CHEN Yin, HE Zheng-quan, LI Hai-ying, ZHUO Ren-ying. Expressed salt stress induced full length-cDNA library of Salix matsudana using yeast Invsc1 . Journal of Zhejiang A&F University, 2009, 26(4): 473-478.
[19]	YANG Qiong-xia, YIN Shu, SHENTU Yin, GAO Xin, SUN Yi, HU Jun-xiang. Radioprotective effects of extract from Carya cathayensis nut exocarp on mice . Journal of Zhejiang A&F University, 2006, 23(6): 604-607.
[20]	HUANG You-jun, WANG Zheng-jia, ZHENG Bing-song, HUANG Jian-qin. Experiment on root cutting of Carya cathayensis . Journal of Zhejiang A&F University, 2006, 23(2): 228-231.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(4569) PDF downloads(1734) Cited by()

Proportional views

Construction of a fat metabolism-related cDNA library in Carya cathayensis

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang ，China

Corresponding author: ZENG Yan-ru

Publish Date: 2011-02-20

Key words:

Abstract: Carya cathayensis is a species with a high oil content in its nuts. In addition，its oil is mostly unsaturated fatty acids. Based on differential display of genes during fruit maturity studied by DDRT-PCR，a fat metabolism-related cDNA library was constructed by SMART. Titering the unamplified library showed a titer of 5.0 105 pfumL－1，an insert size between 0.7 and 2.5 kb and 70.0% of inserts larger than 1.0 kb；and the amplified library had a titer of 5.0 109 pfumL－1，all of the inserts larger than 500 bp，and a recombination rate of 94.5% with 66.0% of the inserts longer than 1 000 bp. This work has laid a foundation for studies of fat-metabolizing mechanism and regulatory network as well as discovery of relevant genes.［Ch，7 fig. 19 ref.］

Construction of a fat metabolism-related cDNA library in Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2011.01.013

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Construction of a fat metabolism-related cDNA library in Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2011.01.013

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang ，China

Corresponding author: ZENG Yan-ru

HTML

Catalog

Construction of a fat metabolism-related cDNA library in Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2011.01.013

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Construction of a fat metabolism-related cDNA library in Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2011.01.013

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang ，China

Corresponding author: ZENG Yan-ru

HTML

Catalog

Export File

Citation

Format

Content