Optimization of a rooting culture system for <em>Rhododendron fortunei</em>

GAO Hang-yang; ZHANG Qi-xiang; HU Heng-kang; ZHANG Zhen-ru; HUANG Jian-qin

doi:10.11833/j.issn.2095-0756.2011.06.024

Volume 28 Issue 6

Dec. 2011

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GAO Hang-yang, ZHANG Qi-xiang, HU Heng-kang, ZHANG Zhen-ru, HUANG Jian-qin. Optimization of a rooting culture system for Rhododendron fortunei[J]. Journal of Zhejiang A&F University, 2011, 28(6): 982-985. doi: 10.11833/j.issn.2095-0756.2011.06.024

Citation:

GAO Hang-yang, ZHANG Qi-xiang, HU Heng-kang, ZHANG Zhen-ru, HUANG Jian-qin. Optimization of a rooting culture system for Rhododendron fortunei[J]. Journal of Zhejiang A&F University, 2011, 28(6): 982-985. doi: 10.11833/j.issn.2095-0756.2011.06.024

Optimization of a rooting culture system for Rhododendron fortunei

doi: 10.11833/j.issn.2095-0756.2011.06.024

1.
School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;
2.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

More Information

Corresponding author: HUANG Jian-qin
Received Date: 2010-11-01
Rev Recd Date: 2011-04-14
Publish Date: 2011-12-20

Abstract

To establish a micro propagation of Rhododendron fortunei，in vitro shoots were used as the explants to establish a rooting system for Rhododendron fortunei. Effects on the basal mediums ［1/2 woody plant medium （WPM），Murashige Skoog （MS），1/2MS，and 1/4MS）］，auxins ［naphthalene-acetic acid （NAA） and indole-3-butyric acid （IBA）］，and sucrose during adventitious root induction were comparatively analyzed by a split plot design experimental design. Results showed that in vitro shoots of R. fortunei inoculated in 1/2 WPM basal medium were significantly higher （P＜0.05） than treatments of MS，1/2 MS，and 1/4 MS basal medium，with a rooting percentage of 100%. In vitro shoots for 1/2 MS basal medium supplemented with 2.0 mgL－1 NAA were significantly higher （P＜0.05） than other NAA treatments with a root percentage of 100%. In vitro shoots in 1/2 MS basal medium supplemented with 0.5 mgL－1 IBA were significantly higher （P＜ 0.05） than other IBA treatments with a root percentage of 100%. The result showed that Rhododendron fortunei the optimal con-centration of sucrose was 5 gL－1，which was significantly higher （P＜0.05） than other sucrose treatments with a rooting percentage of 90.67%. Thus，the optimal rooting medium for R. fortunei was 1/2 WPM + 2.0 mgL－1 NAA + 5.0 gL－1 sucrose. ［Ch，1 fig. 1 tab. 10 ref.］
- botany,
- Rhododendron fortunei,
- in vitro shoots,
- basal medium,
- auxin,
- sucrose

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Optimization of a rooting culture system for Rhododendron fortunei

1. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: HUANG Jian-qin

Received Date: 2010-11-01

Rev Recd Date: 2011-04-14

Publish Date: 2011-12-20

Key words:

Abstract: To establish a micro propagation of Rhododendron fortunei，in vitro shoots were used as the explants to establish a rooting system for Rhododendron fortunei. Effects on the basal mediums ［1/2 woody plant medium （WPM），Murashige Skoog （MS），1/2MS，and 1/4MS）］，auxins ［naphthalene-acetic acid （NAA） and indole-3-butyric acid （IBA）］，and sucrose during adventitious root induction were comparatively analyzed by a split plot design experimental design. Results showed that in vitro shoots of R. fortunei inoculated in 1/2 WPM basal medium were significantly higher （P＜0.05） than treatments of MS，1/2 MS，and 1/4 MS basal medium，with a rooting percentage of 100%. In vitro shoots for 1/2 MS basal medium supplemented with 2.0 mgL－1 NAA were significantly higher （P＜0.05） than other NAA treatments with a root percentage of 100%. In vitro shoots in 1/2 MS basal medium supplemented with 0.5 mgL－1 IBA were significantly higher （P＜ 0.05） than other IBA treatments with a root percentage of 100%. The result showed that Rhododendron fortunei the optimal con-centration of sucrose was 5 gL－1，which was significantly higher （P＜0.05） than other sucrose treatments with a rooting percentage of 90.67%. Thus，the optimal rooting medium for R. fortunei was 1/2 WPM + 2.0 mgL－1 NAA + 5.0 gL－1 sucrose. ［Ch，1 fig. 1 tab. 10 ref.］

Optimization of a rooting culture system for Rhododendron fortunei

doi: 10.11833/j.issn.2095-0756.2011.06.024

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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Proportional views

Optimization of a rooting culture system for Rhododendron fortunei

doi: 10.11833/j.issn.2095-0756.2011.06.024

1. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: HUANG Jian-qin

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Optimization of a rooting culture system for Rhododendron fortunei

doi: 10.11833/j.issn.2095-0756.2011.06.024

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

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Optimization of a rooting culture system for Rhododendron fortunei

doi: 10.11833/j.issn.2095-0756.2011.06.024

1. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China; 2. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: HUANG Jian-qin

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1. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China