Volume 29 Issue 4
Jul.  2012
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XU Yue, SONG Min-guo, YANG Xian-yu, YUAN Jin-qiang. The galectin-3 cDNA diversity and amino acid variation of Bufo gargarizans skin tissue[J]. Journal of Zhejiang A&F University, 2012, 29(4): 574-580. doi: 10.11833/j.issn.2095-0756.2012.04.014
Citation: XU Yue, SONG Min-guo, YANG Xian-yu, YUAN Jin-qiang. The galectin-3 cDNA diversity and amino acid variation of Bufo gargarizans skin tissue[J]. Journal of Zhejiang A&F University, 2012, 29(4): 574-580. doi: 10.11833/j.issn.2095-0756.2012.04.014

The galectin-3 cDNA diversity and amino acid variation of Bufo gargarizans skin tissue

doi: 10.11833/j.issn.2095-0756.2012.04.014
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  • Corresponding author: YANG Xian-yu
  • Received Date: 2011-09-27
  • Rev Recd Date: 2011-11-15
  • Publish Date: 2012-08-20
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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The galectin-3 cDNA diversity and amino acid variation of Bufo gargarizans skin tissue

doi: 10.11833/j.issn.2095-0756.2012.04.014
    Corresponding author: YANG Xian-yu

Abstract: To obtain the cDNA clone of galectin-3,a first-strand cDNA library was synthesized from the total RNA of Bufo gargarizans skin and then used as a template for subsequent polymerase chain reaction (PCR) amplification. The PCR product was successfully cloned into pGM-T vector. After sequencing and using the National Center for Biotechnology Information (NCBI),an open reading frame (ORF) finder was utilized to determine the cDNA. Then,Clustal X 2.0 and DnaSP 4 software were used to analyze cDNA diversity and the deduced amino acid variation. Results of the ORF finder showed nine cDNA clones of galectin-3,two of which were estimated to encode truncated protein. Clustal X 2.0 and DnaSP 4.0 revealed a high density of single nucleotide polymorphism (SNP) with an average of 1 SNP per 20 bp. For deduced amino acid residues,the N-terminal and carbohydrate recognition domain (CRD) were highly consistent with a phosphorylation site,a CRD,and two related motifs. Meanwhile,the tandem repeated domain showed strong differences. Thus,this study indicated a molecular basis for bio-adaptation of B. gargarizans in certain habitat.[Ch,3 fig. 1 tab. 24 ref.]

XU Yue, SONG Min-guo, YANG Xian-yu, YUAN Jin-qiang. The galectin-3 cDNA diversity and amino acid variation of Bufo gargarizans skin tissue[J]. Journal of Zhejiang A&F University, 2012, 29(4): 574-580. doi: 10.11833/j.issn.2095-0756.2012.04.014
Citation: XU Yue, SONG Min-guo, YANG Xian-yu, YUAN Jin-qiang. The galectin-3 cDNA diversity and amino acid variation of Bufo gargarizans skin tissue[J]. Journal of Zhejiang A&F University, 2012, 29(4): 574-580. doi: 10.11833/j.issn.2095-0756.2012.04.014

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