The galectin-3 cDNA diversity and amino acid variation of <em>Bufo gargarizans</em> skin tissue

XU Yue; SONG Min-guo; YANG Xian-yu; YUAN Jin-qiang

doi:10.11833/j.issn.2095-0756.2012.04.014

Volume 29 Issue 4

Jul. 2012

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XU Yue, SONG Min-guo, YANG Xian-yu, YUAN Jin-qiang. The galectin-3 cDNA diversity and amino acid variation of Bufo gargarizans skin tissue[J]. Journal of Zhejiang A&F University, 2012, 29(4): 574-580. doi: 10.11833/j.issn.2095-0756.2012.04.014

Citation:

XU Yue, SONG Min-guo, YANG Xian-yu, YUAN Jin-qiang. The galectin-3 cDNA diversity and amino acid variation of Bufo gargarizans skin tissue[J]. Journal of Zhejiang A&F University, 2012, 29(4): 574-580. doi: 10.11833/j.issn.2095-0756.2012.04.014

The galectin-3 cDNA diversity and amino acid variation of Bufo gargarizans skin tissue

doi: 10.11833/j.issn.2095-0756.2012.04.014

1.
School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

More Information

Corresponding author: YANG Xian-yu
Received Date: 2011-09-27
Rev Recd Date: 2011-11-15
Publish Date: 2012-08-20

Abstract

To obtain the cDNA clone of galectin-3，a first-strand cDNA library was synthesized from the total RNA of Bufo gargarizans skin and then used as a template for subsequent polymerase chain reaction （PCR） amplification. The PCR product was successfully cloned into pGM-T vector. After sequencing and using the National Center for Biotechnology Information （NCBI），an open reading frame （ORF） finder was utilized to determine the cDNA. Then，Clustal X 2.0 and DnaSP 4 software were used to analyze cDNA diversity and the deduced amino acid variation. Results of the ORF finder showed nine cDNA clones of galectin-3，two of which were estimated to encode truncated protein. Clustal X 2.0 and DnaSP 4.0 revealed a high density of single nucleotide polymorphism （SNP） with an average of 1 SNP per 20 bp. For deduced amino acid residues，the N-terminal and carbohydrate recognition domain （CRD） were highly consistent with a phosphorylation site，a CRD，and two related motifs. Meanwhile，the tandem repeated domain showed strong differences. Thus，this study indicated a molecular basis for bio-adaptation of B. gargarizans in certain habitat.［Ch，3 fig. 1 tab. 24 ref.］
- zoology,
- Bufo gargarizans,
- galectin-3,
- diversity,
- bio-adaptation

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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The galectin-3 cDNA diversity and amino acid variation of Bufo gargarizans skin tissue

1. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: YANG Xian-yu

Received Date: 2011-09-27

Rev Recd Date: 2011-11-15

Publish Date: 2012-08-20

Key words:

Abstract: To obtain the cDNA clone of galectin-3，a first-strand cDNA library was synthesized from the total RNA of Bufo gargarizans skin and then used as a template for subsequent polymerase chain reaction （PCR） amplification. The PCR product was successfully cloned into pGM-T vector. After sequencing and using the National Center for Biotechnology Information （NCBI），an open reading frame （ORF） finder was utilized to determine the cDNA. Then，Clustal X 2.0 and DnaSP 4 software were used to analyze cDNA diversity and the deduced amino acid variation. Results of the ORF finder showed nine cDNA clones of galectin-3，two of which were estimated to encode truncated protein. Clustal X 2.0 and DnaSP 4.0 revealed a high density of single nucleotide polymorphism （SNP） with an average of 1 SNP per 20 bp. For deduced amino acid residues，the N-terminal and carbohydrate recognition domain （CRD） were highly consistent with a phosphorylation site，a CRD，and two related motifs. Meanwhile，the tandem repeated domain showed strong differences. Thus，this study indicated a molecular basis for bio-adaptation of B. gargarizans in certain habitat.［Ch，3 fig. 1 tab. 24 ref.］

The galectin-3 cDNA diversity and amino acid variation of Bufo gargarizans skin tissue

doi: 10.11833/j.issn.2095-0756.2012.04.014

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The galectin-3 cDNA diversity and amino acid variation of Bufo gargarizans skin tissue

doi: 10.11833/j.issn.2095-0756.2012.04.014

1. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: YANG Xian-yu

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The galectin-3 cDNA diversity and amino acid variation of Bufo gargarizans skin tissue

doi: 10.11833/j.issn.2095-0756.2012.04.014

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通讯作者: 陈斌, bchen63@163.com

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The galectin-3 cDNA diversity and amino acid variation of Bufo gargarizans skin tissue

doi: 10.11833/j.issn.2095-0756.2012.04.014

1. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: YANG Xian-yu

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